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    CULTURE DEVICE AND METHOD FOR EUKARYOTIC CELL TRANSFECTION
    3.
    发明公开
    CULTURE DEVICE AND METHOD FOR EUKARYOTIC CELL TRANSFECTION 有权
    培养装置和方法真核细胞的转染

    公开(公告)号：EP1723240A1

    公开(公告)日：2006-11-22

    申请号：EP04712389.8

    申请日：2004-02-18

    申请人： NITTO DENKO CORPORATION

    发明人： TANAKA, Yasunobu ,  YU, Lei ,  JI, Shouping

    IPC分类号： C12N15/64

    CPC分类号： C12M23/12 ,  C12M35/00 ,  C12N15/87

    摘要： A cell culture device for transfecting a eukaryotic cell is disclosed. The cell culture/transfection device may be a multiwell plate or slide which has been coated with a metal salt such as CaCl2. Methods of using the cell culture device to transfect mammalian cells and /or to monitor cell transfection are described. Kits are also described which include the cell transfection device, eukaryotic cells to be transformed and nucleic acid for transformation.

    BIODEGRADABLE POLYACETALS
    5.
    发明公开
    BIODEGRADABLE POLYACETALS 审中-公开
    可生物降解的聚缩醛

    公开(公告)号：EP1601721A1

    公开(公告)日：2005-12-07

    申请号：EP04714119.7

    申请日：2004-02-24

    申请人： NITTO DENKO CORPORATION

    发明人： YU, Lei ,  VAN, Sang ,  JI, Shouping ,  MATSUMOTO, Kenji

    IPC分类号： C08L59/00 ,  C08L59/02 ,  C08G4/00 ,  C08G2/00 ,  C08G73/02

    CPC分类号： C08G73/0213 ,  A61K47/59 ,  C08G65/002

    摘要： A polymer comprising recurring units represented by formula (I); wherein X is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VZ, where R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl, where V is a labile linker group, and where Z is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, and hexadecaamine dendrimer; and wherein Y is selected from the group consisting of -(CH2)2-, -(CH2)2-O-(CH2)2-, -(CH2)2-O-(CH2)2-O-(CH2)2-, and -(CH2)3-NHC(O)-(CH2)6-C(O)NH-(CH2)3- is useful in nucleic acid delivery applications. Polyacetals of the formula (I) are preferably made by reacting appropriate diols and divinyl ethers. In preferred embodiments, complexes formed between polyacetals of the formula (I) and polynucleotides are useful as transfection reagents.

    ACID-SENSITIVE POLYACETALS AND METHODS
    7.
    发明授权
    ACID-SENSITIVE POLYACETALS AND METHODS 有权
    酸敏感聚缩醛和方法

    公开(公告)号：EP1664144B1

    公开(公告)日：2011-04-27

    申请号：EP04779365.8

    申请日：2004-07-28

    申请人： NITTO DENKO CORPORATION

    发明人： VAN, Sang ,  VIROONCHATAPAN, Nitnara ,  JI, Shouping ,  YU, Lei

    IPC分类号： C08G4/00 ,  C08L59/00

    CPC分类号： C08G4/00 ,  C07C229/22 ,  C08G73/028

    摘要： Polyacetals comprising a recurring units represented by a formula selected from the group consisting of formula (I) and (II): wherein A comprises at least one acetal group; B is selected from the group consisting of -CH-, -CH(CH3)-, CH2CH-, -CH2C(CH3)-, -CH(CH3)CH-, and -CHCH(CH3)CH(CH3)-; Z is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VU; V is a linker group; U is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, hexadecaamine dendrimer, enhancer, and targeting receptor; R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl; D is a linkage selected from the group consisting of carboxylic amide, carboxylic ester, urea, and urethane; and G is selected from the group consisting of C4 to C20 alkyl, C6-C10 aryl, and -(OCH2CH2)n-, where n is in the range of 1 to about 250, are useful in nucleic acid delivery applications. In preferred embodiments, complexes formed between polyacetals comprising recurring units of the formulae (I) or (II) and polynucleotides are useful as transfection reagents.

    ACID-SENSITIVE POLYACETALS AND METHODS
    8.
    发明公开
    ACID-SENSITIVE POLYACETALS AND METHODS 有权
    酸敏感聚缩醛和方法

    公开(公告)号：EP1664144A1

    公开(公告)日：2006-06-07

    申请号：EP04779365.8

    申请日：2004-07-28

    申请人： NITTO DENKO CORPORATION

    发明人： VAN, Sang ,  VIROONCHATAPAN, Nitnara ,  JI, Shouping ,  YU, Lei

    IPC分类号： C08G4/00 ,  C08L59/00

    CPC分类号： C08G4/00 ,  C07C229/22 ,  C08G73/028

    摘要： Polyacetals comprising a recurring units represented by a formula selected from the group consisting of formula (I) and (II): wherein A comprises at least one acetal group; B is selected from the group consisting of -CH-, -CH(CH3)-, CH2CH-, -CH2C(CH3)-, -CH(CH3)CH-, and -CHCH(CH3)CH(CH3)-; Z is selected from the group consisting of C(O)OR1, C(O)SR1, C(O)NR1R2, and VU; V is a linker group; U is selected from the group consisting of poly(ethyleneimine), poly(propyleneimine), poly(lysine), PAMAM dendrimer, octaamine dendrimer, hexadecaamine dendrimer, enhancer, and targeting receptor; R1 and R2 are each individually selected from the group consisting of hydrogen, C1 to C10 alkyl, and C6 to C10 aryl; D is a linkage selected from the group consisting of carboxylic amide, carboxylic ester, urea, and urethane; and G is selected from the group consisting of C4 to C20 alkyl, C6-C10 aryl, and -(OCH2CH2)n-, where n is in the range of 1 to about 250, are useful in nucleic acid delivery applications. In preferred embodiments, complexes formed between polyacetals comprising recurring units of the formulae (I) or (II) and polynucleotides are useful as transfection reagents.