摘要:
A cell cultivation method includes cultivating kidney cells 24 in a state of being non-adherent to a culture vessel 26 for a period of 5 days or longer, forming aggregates 30 of the kidney cells 24 during the cultivation period, and then cultivating the kidney cells 24 in a state of having formed aggregates 30, during a portion of the period.
摘要:
A microbial mat is provided, including a contact layer; a core layer; a matrix of cellulose fibers coated with a liquid nutrient mix; and a carrying sheet; wherein the core layer comprises the matrix of cellulose fibers coated with a liquid nutrient mix, attached to the carrying sheet and coated with a dry nutrient mix; wherein the liquid nutrient mix and dry nutrient mix are composed of ingredients from the group consisting of Sodium Citrate, Ammonium Sulphate, Monosodium phosphate, Dipotassium Phosphate, Magnesium Sulphate, Eprom Salt, and Ferrous Sulphate; and a top layer, wherein the top layer is attached to the entire surface of the core layer.
摘要:
The invention relates to an apparatus (1) for culturing anchorage-dependent cells. The apparatus (1) comprises a housing (2) with an inlet (4) and an outlet (5), and a plurality of culture plates (6) removably stacked within the housing (2). The housing (2) has a circumferential wall (7), a base (8) and a top wall (9). The base (8) comprises the inlet (4) and the top wall (9) comprises the outlet (5). The circumferential wall (7) of the housing (2) defines a longitudinal axis thereof, as well as an inner cross section perpendicular to the longitudinal axis. Shape and dimensions of the inner cross section are essentially uniform along the longitudinal axis. The culture plates (6) are arranged at least essentially parallel to each other. Each plate (6) is mounted and sealed to the circumferential wall (7) of the housing (2). Plates (6) are arranged at a distance from each other. Each culture plate (6) has a through hole (14), so that inlet (4) and outlet (5) are in fluid communication. Each through hole (14) is positioned at an outer end of the respective culture plate (6), proximate the circumferential wall (7). Through holes (14) of adjacent culture plates (6) are distally positioned in the plane of the inner cross section of the housing (2).
摘要:
The present invention relates to a method of using cells to produce a substance, and involves applying the cells to a polyimide porous film, culturing the cells and producing the substance by means of the cells.