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    A PROCESS FOR ISOLATION AND PURIFICATION OF A TARGET PROTEIN FREE OF PRION PROTEIN (PRPSC)
    6.
    发明公开
    A PROCESS FOR ISOLATION AND PURIFICATION OF A TARGET PROTEIN FREE OF PRION PROTEIN (PRPSC) 有权
    VERFAHREN ZUR ISOLIERUNG REINIGUNG EINES PRIONENPROTEINFREIEN TARGET-PROTEINS（PRPSC）

    公开(公告)号：EP3034097A1

    公开(公告)日：2016-06-22

    申请号：EP15198333.5

    申请日：2008-08-25

    申请人： Octapharma AG

    发明人： GILLJAM, Gustav ,  JERNBERG, Mats ,  WINGE, Stefan ,  NEISSER-SVAE, Andrea

    IPC分类号： A61L2/00 ,  C07K14/47 ,  G01N33/68 ,  C07K1/16

    CPC分类号： C07K1/22 ,  B01D15/3828 ,  B01D15/426 ,  C07K14/47

    摘要： A process for isolation and purification of a target protein by chromatography wherein the chromatography removes or depletes prions (PrP Sc ), comprising the steps of
    - contacting a potentially PrP SC -contaminated sample comprising a target protein with a multimodal chromatographic material;
    - setting buffer conditions so that the target protein is bound to the multimodal chromatographic material and whereas PrP SC is not binding to the multimodal chromatographic material;
    - followed by elution of the target protein.

    摘要翻译： 用于通过色谱分离和纯化靶蛋白的方法，其中所述色谱法除去或消除朊病毒（PrP Sc），其包括以下步骤：将包含靶蛋白的潜在PrP SC-污染的样品与多峰色谱材料接触; - 设置缓冲液条件，使靶蛋白与多峰色谱材料结合，而PrP SC不与多峰色谱材料结合; - 然后洗脱靶蛋白。

    A PROCESS FOR PURIFYING VITAMIN K DEPENDENT PROTEINS
    10.
    发明公开
    A PROCESS FOR PURIFYING VITAMIN K DEPENDENT PROTEINS 审中-公开
    VERFAHREN ZUR REINIGUNG VON维生素K-ABHÄNGIGEN蛋白

    公开(公告)号：EP3133157A1

    公开(公告)日：2017-02-22

    申请号：EP16190737.3

    申请日：2011-03-30

    申请人： Octapharma AG

    发明人： GILLJAM, Gustav ,  WINGE, Stefan

    IPC分类号： C12N9/64

    CPC分类号： C07K1/22 ,  C07K14/745 ,  C12N9/6424

    摘要： A process of manufacturing a prion-free Vitamin K dependent Protein in a purification sequence employing chromatography characterized in that
    - at least one chromatography step is performed using a multimodal resin
    - providing a fraction containing Vitamin K dependent Protein in an aqueous solution;
    - contacting the fraction containing the Vitamin K dependent Protein with a multimodal resin at a pH between 6-9;
    - optionally washing the multimodal resin having the Vitamin K dependent Protein adsorbed with an aqueous washing buffer to wash away contaminants and retain the Vitamin K dependent Protein, before the Vitamin K dependent Protein is eluted;
    - the Vitamin K dependent Protein is eluting from the multimodal resin at a pH between 6 to 9 in a buffer comprising arginine; and
    - optionally collecting Vitamin K dependent Protein containing fractions in purified or enriched form.

    摘要翻译： 在使用色谱法的纯化序列中制备无朊病毒的维生素K依赖性蛋白质的方法，其特征在于 - 使用多峰树脂进行至少一个色谱步骤 - 在水溶液中提供含有维生素K依赖性蛋白质的级分; - 将含有维生素K依赖性蛋白质的级分与多峰树脂在6-9之间的pH下接触; - 在维生素K依赖性蛋白质被洗脱之前，任选地洗涤具有维生素K依赖性蛋白质的多峰树脂，其吸附有水性洗涤缓冲液以洗去污染物并保留维生素K依赖性蛋白质; - 维生素K依赖性蛋白质在含有精氨酸的缓冲液中从6至9的pH在多峰树脂中洗脱; 和 - 任选地以纯化或富集的形式收集含维生素K依赖性蛋白质的级分。