公开(公告)号：EP1709068A2

公开(公告)日：2006-10-11

申请号：EP04814870.4

申请日：2004-12-20

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. du Pont de Nemours and Company

发明人： ABAD, André ,  FLANNAGAN, Ronald, D. ,  HERRMANN, Rafael ,  LU, Albert ,  MCCUTCHEN, Billy, F. ,  PRESNAIL, James, K. ,  RICE, Janet, A. ,  WONG, James, F. ,  YU, Cao-Guo

IPC分类号： C07K14/325 ,  C12N9/64 ,  C12N15/82 ,  A01H5/02

CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

公开(公告)号：EP1572880B1

公开(公告)日：2014-01-22

申请号：EP02756376.6

申请日：2002-06-21

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： CAHOON, Rebecca, E. ,  HERRMANN, Rafael ,  HARVELL, Leslie, T. ,  LU, Albert, Laurence ,  MCCUTCHEN, Billy, Fred ,  NAVARRO ACEVEDO, Pedro, A. ,  SIMMONS, Carl, R. ,  WONG, James, F., H.

IPC分类号： C12N15/29 ,  C12N15/82 ,  C07K14/415

CPC分类号： C07K14/4723 ,  C07K14/415 ,  C12N15/8279

公开(公告)号：EP1763536A1

公开(公告)日：2007-03-21

申请号：EP05788923.0

申请日：2005-07-01

申请人： Pioneer-Hi-Bred International, Inc. ,  E.I. du Pont de Nemours and Company ,  The Regents of the University of California

发明人： ALTIER, Daniel, J., Altier ,  DAHLBACKA, Glen ,  HERRMANN, Rafael ,  HUNTER-CEVERA, Jennie ,  McCUTCHEN, Billy, Fred ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  RICE, Janet, A. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLESKAYA, I., A.

IPC分类号： C07K14/385 ,  C07K14/37

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.

公开(公告)号：EP1572880A3

公开(公告)日：2005-12-07

申请号：EP02756376.6

申请日：2002-06-21

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： CAHOON, Rebecca, E. ,  HERRMANN, Rafael ,  HARVELL, Leslie, T. ,  LU, Albert, Laurence ,  MCCUTCHEN, Billy, Fred ,  NAVARRO ACEVEDO, Pedro, A. ,  SIMMONS, Carl, R. ,  WONG, James, F., H.

IPC分类号： C12N1/00

公开(公告)号：EP1699816A2

公开(公告)日：2006-09-13

申请号：EP04813794.7

申请日：2004-12-09

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. du Pont de Nemours and Company

发明人： ABAD, André ,  DONG, Hua ,  HERRMANN, Rafael ,  LU, Albert ,  McCUTCHEN, Billy, F. ,  RICE, Janet, A. ,  SCHEPERS, Eric, J. ,  WONG, James, F.

IPC分类号： C07K14/325 ,  C12N15/82

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide expression cassettes and transformed plants, plant cells, and seeds. These compositions find use in methods for protecting a plant from a pest.

公开(公告)号：EP1893638B1

公开(公告)日：2011-08-10

申请号：EP06772278.5

申请日：2006-06-06

申请人： Pioneer-Hi-Bred International, Inc. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： ABAD, Andre R. ,  CHUN, Gary C. ,  FLANNAGAN, Ronald D. ,  HERRMANN, Rafael ,  LU, Albert L. ,  MCCUTCHEN, Billy F. ,  RICE, Janet A. ,  SCHEPERS, Eric J.

IPC分类号： C07K14/325 ,  C12N9/64 ,  A01N63/02 ,  C12N15/82

CPC分类号： C12N15/8286 ,  A01N63/02 ,  C07K14/325 ,  C12N9/6472 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.

公开(公告)号：EP1572880A2

公开(公告)日：2005-09-14

申请号：EP02756376.6

申请日：2002-06-21

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. DU PONT DE NEMOURS AND COMPANY

发明人： CAHOON, Rebecca, E. ,  HERRMANN, Rafael ,  HARVELL, Leslie, T. ,  LU, Albert, Laurence ,  MCCUTCHEN, Billy, Fred ,  NAVARRO ACEVEDO, Pedro, A. ,  SIMMONS, Carl, R. ,  WONG, James, F., H.

IPC分类号： C12N1/00

CPC分类号： C07K14/4723 ,  C07K14/415 ,  C12N15/8279

摘要： Methods and compositions for mudulating development and defense responses are provided. Nucleotide sequences encoding defensin proteins are provided. The sequences can be used in expression cassettes for mudulating development, developmental pathways, and defense responses. Transformed plants, plant cells, tissues, and seed are also provided.

公开(公告)号：EP1185653B1

公开(公告)日：2008-08-27

申请号：EP00943005.9

申请日：2000-06-21

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： HERRMANN, Rafael ,  LEE, Jian-Ming ,  WONG, James, F.

IPC分类号： C12N15/12 ,  C07K14/435 ,  C12N15/86 ,  C12N1/00 ,  C12N15/82 ,  G01N33/68 ,  C12Q1/68

CPC分类号： C12N15/86 ,  C07K14/43522 ,  C12N2710/14143

公开(公告)号：EP1797116A2

公开(公告)日：2007-06-20

申请号：EP05803784.7

申请日：2005-10-04

申请人： E.I. du Pont de Nemours and Company

发明人： HERRMANN, Rafael ,  LU, Albert L. ,  MCCUTCHEN, Billy F. ,  PRESNAIL, James K. ,  RICE, Janet A. ,  WAINE, Clement W. K.

IPC分类号： C07K14/415

CPC分类号： C07K14/415 ,  C12N15/8286 ,  Y02A40/162

摘要： The present invention relates to isolated nucleic acids encoding plant cyclotides. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant cyclotides, in sense or antisense orientation, wherein expression of the chimeric gene results in the production of altered levels of plant cyclotides in a transformed host cell.

公开(公告)号：EP1534829B1

公开(公告)日：2008-12-31

申请号：EP03739278.4

申请日：2003-06-20

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： FAMODU, Omolayo, O. ,  HERRMANN, Rafael ,  LU, Albert, L. ,  MCCUTCHEN, Billy, Fred ,  MIAO, Guo-Hua ,  PRESNAIL, James, K. ,  WENG, Zude

IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8279 ,  C07K14/415 ,  C12N15/8282 ,  C12N15/8285 ,  Y02A40/164

摘要： This invention relates to isolated nucleic acids encoding plant defensins. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant defensin, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of plant defensins in a transformed host cell.