公开(公告)号：EP2027276B1

公开(公告)日：2011-02-02

申请号：EP07845231.5

申请日：2007-05-15

申请人： Pioneer Hi-Bred International, Inc. ,  E.I. DU PONT DE NEMOURS AND COMPANY ,  The Regents of the University of California

发明人： ALTIER, Daniel, J. ,  CRANE, Virginia, C. ,  GILLIAM, Jacob, T. ,  HUNTER-CEVERA, Jennie ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLANSKAYA, I.A.

IPC分类号： C12N15/82 ,  C07K14/385

CPC分类号： C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

公开(公告)号：EP2027276A2

公开(公告)日：2009-02-25

申请号：EP07845231.5

申请日：2007-05-15

申请人： Pioneer Hi-Bred International Inc. ,  E.I. DU PONT DE NEMOURS AND COMPANY ,  The Regents of the University of California

发明人： ALTIER, Daniel, J. ,  CRANE, Virginia, C. ,  GILLIAM, Jacob, T. ,  HUNTER-CEVERA, Jennie ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLANSKAYA, I.A.

IPC分类号： C12N15/82 ,  C07K14/385

CPC分类号： C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.

公开(公告)号：EP1709068A2

公开(公告)日：2006-10-11

申请号：EP04814870.4

申请日：2004-12-20

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E.I. du Pont de Nemours and Company

发明人： ABAD, André ,  FLANNAGAN, Ronald, D. ,  HERRMANN, Rafael ,  LU, Albert ,  MCCUTCHEN, Billy, F. ,  PRESNAIL, James, K. ,  RICE, Janet, A. ,  WONG, James, F. ,  YU, Cao-Guo

IPC分类号： C07K14/325 ,  C12N9/64 ,  C12N15/82 ,  A01H5/02

CPC分类号： C12N15/8286 ,  C12N9/641 ,  Y02A40/162

摘要： Compositions and methods for protecting a plant from an insect pest are provided. In particular, nucleic acid sequences encoding insect protoxins modified to comprise at least one proteolytic activation site that is sensitive to a plant protease or an insect gut protease are provided. Cleavage of the modified protoxin at the proteolytic activation site by a protease produces an active insect toxin. Methods of using the modified insect protoxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds.

公开(公告)号：EP1534829A2

公开(公告)日：2005-06-01

申请号：EP03739278.4

申请日：2003-06-20

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： FAMODU, Omolayo, O. ,  HERRMANN, Rafael ,  LU, Albert, L. ,  MCCUTCHEN, Billy, Fred ,  MIAO, Guo-Hua ,  PRESNAIL, James, K. ,  WENG, Zude

IPC分类号： C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8279 ,  C07K14/415 ,  C12N15/8282 ,  C12N15/8285 ,  Y02A40/164

摘要： This invention relates to isolated nucleic acids encoding plant defensins. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant defensin, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of plant defensins in a transformed host cell.

公开(公告)号：EP1531664A2

公开(公告)日：2005-05-25

申请号：EP03742192.2

申请日：2003-06-25

申请人： E.I. du Pont de Nemours and Company

发明人： ABAD, Andre, R. ,  FLANNAGAN, Ronald, D. ,  HERRMANN, Rafael ,  KAHN, Theodore, W. ,  LU, Albert, Laurence ,  MCCUTCHEN, Billy Fred ,  PRESNAIL, James, K. ,  WONG, James, F., H. ,  YU, Cao-Guo

IPC分类号： A01H5/00 ,  A01H5/10 ,  C12N15/82 ,  C12N15/32 ,  C07K14/325 ,  C12Q1/25

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding delta-endotoxins having pesticidal activity against insect pests. The invention further provides mutagenized nucleic acids that have been modified to encode pesticidal polypeptides such as endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins that may be optimized as well as pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

公开(公告)号：EP1534829B1

公开(公告)日：2008-12-31

申请号：EP03739278.4

申请日：2003-06-20

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： FAMODU, Omolayo, O. ,  HERRMANN, Rafael ,  LU, Albert, L. ,  MCCUTCHEN, Billy, Fred ,  MIAO, Guo-Hua ,  PRESNAIL, James, K. ,  WENG, Zude

IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/8279 ,  C07K14/415 ,  C12N15/8282 ,  C12N15/8285 ,  Y02A40/164

摘要： This invention relates to isolated nucleic acids encoding plant defensins. The invention also relates to the construction of a chimeric gene encoding all or a portion of the plant defensin, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of plant defensins in a transformed host cell.

公开(公告)号：EP1763536A1

公开(公告)日：2007-03-21

申请号：EP05788923.0

申请日：2005-07-01

申请人： Pioneer-Hi-Bred International, Inc. ,  E.I. du Pont de Nemours and Company ,  The Regents of the University of California

发明人： ALTIER, Daniel, J., Altier ,  DAHLBACKA, Glen ,  HERRMANN, Rafael ,  HUNTER-CEVERA, Jennie ,  McCUTCHEN, Billy, Fred ,  PRESNAIL, James, K. ,  SCHEPERS, Eric, J. ,  RICE, Janet, A. ,  SIMMONS, Carl, R. ,  TOROK, Tamas ,  YALPANI, Nasser ,  ELLESKAYA, I., A.

IPC分类号： C07K14/385 ,  C07K14/37

CPC分类号： C07K14/37 ,  A01N63/02 ,  A01N63/04 ,  A61K38/00 ,  C07K14/385 ,  C12N15/8282

摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include novel amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from microbial fermentation broths. Nucleic acid molecules comprising nucleotide sequences that encode the antipathogenic polypeptides of the invention are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention, or variant or fragment thereof, are also disclosed.