公开(公告)号：EP2608878A2

公开(公告)日：2013-07-03

申请号：EP11820522.8

申请日：2011-08-23

申请人： President and Fellows of Harvard College ,  Universität Augsburg

发明人： WEITZ, David, A. ,  FRANKE, Thomas ,  WIXFORTH, Achim ,  SCHMID, Lothar ,  AGRESTI, Jeremy ,  ABATE, Adam, R.

IPC分类号： B01J19/10 ,  C12M1/42

CPC分类号： C12M23/16 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/0421 ,  B01L2400/0436 ,  B01L2400/0487 ,  B01L2400/0496 ,  C12M47/04 ,  F17D3/01 ,  G01N2015/1006 ,  G01N2015/1081 ,  Y10T137/0391

摘要： Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.

摘要翻译： 本发明的各个方面涉及流体物质的控制和操作，例如在微流体系统中。 在一组实施例中，可以使用表面声波对液滴进行分类。 液滴可能含有细胞或其他物种。 在一些情况下，表面声波可以使用表面声波发生器（例如叉指传感器）和/或诸如压电基片的材料来产生。 压电衬底可以与微流体衬底隔离，除了在液滴被分类的位置处或附近，例如，进入第一或第二微流体通道。 在这样的位置处，微流体衬底可以通过一个或多个耦合区域耦合到压电衬底（或其它材料）。 在一些情况下，可以例如以至少约1000Hz，至少约10,000Hz或至少约100,000Hz的速率，并且在一些实施方案中，在分选后具有高的细胞活力来实现相对较高的分选率。

公开(公告)号：EP3341116A1

公开(公告)日：2018-07-04

申请号：EP16840074.5

申请日：2016-08-25

申请人： President and Fellows of Harvard College

发明人： WEITZ, David, A. ,  UNG, Warren, Lioyd ,  FRANKE, Thomas

IPC分类号： B01J19/10 ,  B01L3/00 ,  G01N29/02

CPC分类号： B01L3/502761 ,  B01L3/502715 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/0436 ,  B01L2400/086 ,  G01N15/0255

摘要： The present invention generally relates to the manipulation of species using acoustic waves such as surface acoustic waves. In some aspects, a channel such as a microfluidic channel may be provided having two or more outlets, and acoustic waves applied to species within the channel to determine which outlet the species is directed to. For instance, surface acoustic waves may be applied to a species such as a cell or a particle to deflect it from the channel into a groove or other portion that directs it to a different outlet. In some cases, surprisingly, this deflection of species may be in a different direction than the incident acoustic waves on the channel. Other embodiments of the present invention are generally directed to kits including such systems, techniques for producing such systems, or the like.