利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

72 条记录 (耗时 0.043 秒)

    ACOUSTIC WAVES IN MICROFLUIDICS
    1.
    发明公开
    ACOUSTIC WAVES IN MICROFLUIDICS 审中-公开
    SCHERWELLEN IN DER MIKROFLUIDIK

    公开(公告)号：EP2608878A2

    公开(公告)日：2013-07-03

    申请号：EP11820522.8

    申请日：2011-08-23

    申请人： President and Fellows of Harvard College ,  Universität Augsburg

    发明人： WEITZ, David, A. ,  FRANKE, Thomas ,  WIXFORTH, Achim ,  SCHMID, Lothar ,  AGRESTI, Jeremy ,  ABATE, Adam, R.

    IPC分类号： B01J19/10 ,  C12M1/42

    CPC分类号： C12M23/16 ,  B01L3/502761 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2400/0421 ,  B01L2400/0436 ,  B01L2400/0487 ,  B01L2400/0496 ,  C12M47/04 ,  F17D3/01 ,  G01N2015/1006 ,  G01N2015/1081 ,  Y10T137/0391

    摘要： Various aspects of the present invention relate to the control and manipulation of fluidic species, for example, in microfluidic systems. In one set of embodiments, droplets may be sorted using surface acoustic waves. The droplets may contain cells or other species. In some cases, the surface acoustic waves may be created using a surface acoustic wave generator such as an interdigitated transducer, and/or a material such as a piezoelectric substrate. The piezoelectric substrate may be isolated from the microfluidic substrate except at or proximate the location where the droplets are sorted, e.g., into first or second microfluidic channels. At such locations, the microfluidic substrate may be coupled to the piezoelectric substrate (or other material) by one or more coupling regions. In some cases, relatively high sorting rates may be achieved, e.g., at rates of at least about 1,000 Hz, at least about 10,000 Hz, or at least about 100,000 Hz, and in some embodiments, with high cell viability after sorting.

    摘要翻译： 本发明的各个方面涉及流体物质的控制和操作，例如在微流体系统中。 在一组实施例中，可以使用表面声波对液滴进行分类。 液滴可能含有细胞或其他物种。 在一些情况下，表面声波可以使用表面声波发生器（例如叉指传感器）和/或诸如压电基片的材料来产生。 压电衬底可以与微流体衬底隔离，除了在液滴被分类的位置处或附近，例如，进入第一或第二微流体通道。 在这样的位置处，微流体衬底可以通过一个或多个耦合区域耦合到压电衬底（或其它材料）。 在一些情况下，可以例如以至少约1000Hz，至少约10,000Hz或至少约100,000Hz的速率，并且在一些实施方案中，在分选后具有高的细胞活力来实现相对较高的分选率。

    SYSTEMS AND METHODS FOR BARCODING NUCLEIC ACIDS
    6.
    发明公开
    SYSTEMS AND METHODS FOR BARCODING NUCLEIC ACIDS 审中-公开
    系统VERFAHREN ZUR BARCODIERUNG VONNUKLEINSÄUREN

    公开(公告)号：EP3134536A1

    公开(公告)日：2017-03-01

    申请号：EP15783629.7

    申请日：2015-04-17

    申请人： President and Fellows of Harvard College ,  Vilnius University

    发明人： WEITZ, David, A. ,  KLEIN, Allon, Moshe ,  AKARTUNA, Ilke ,  MAZUTIS, Linas ,  KIRSCHNER, Marc, W.

    IPC分类号： C12P19/34

    摘要： The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include "barcodes" or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.

    摘要翻译： 本发明一般涉及微流体和标记的核酸。 例如，某些方面通常涉及用于在微流体液滴内标记核酸的系统和方法。 在一组实施方案中，核酸可以包括可用于将液滴中的核酸与另一个液滴中的核酸区分开的“条形码”或唯一序列，例如甚至在核酸汇集在一起​​之后。 在一些情况下，可以使用颗粒将独特的序列并入单独的液滴中并连接到液滴内的核酸（例如从裂解的细胞中释放）。 在一些情况下，条形码可以用于区分数十，数百甚至数千个核酸，例如由不同细胞或其他来源产生的核酸。

    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION
    7.
    发明公开
    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION 审中-公开
    VERFAHREN UND SYSTEME ZURTRÖPFCHENMARKIERUNGUND-EXGRÖSSERUNG

    公开(公告)号：EP3132037A1

    公开(公告)日：2017-02-22

    申请号：EP15780364.4

    申请日：2015-04-17

    申请人： President and Fellows of Harvard College ,  The General Hospital Corporation d/b/a Massachusetts General Hospital

    发明人： WEITZ, David, A. ,  IAFRATE, Anthony J. ,  ZHENG, Zongli ,  ZHUANG, Huidan

    IPC分类号： C12N15/10

    CPC分类号： C12N15/1065 ,  C12N15/10 ,  C12N15/1037 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    摘要： The present invention generally relates to microfluidic devices, including methods and systems for tagging droplets within such devices. In some aspects, microfluidic droplets are manipulated by exposing the droplets (or other discrete entities) to a variety of different conditions. By incorporating into the droplets a plurality of nucleic acid "tags," and optionally amplifying the nucleic acids, e.g., within the droplets, the conditions that a droplet was exposed to may be encoded by the nucleic acids. Thus, even if droplets exposed to different conditions are mixed together, the conditions that each droplet encountered may still be determined, for example, by sequencing the nucleic acids.

    摘要翻译： 本发明一般涉及微流体装置，包括用于在这些装置内标记液滴的方法和系统。 在一些方面，通过将液滴（或其他离散实体）暴露于各种不同的条件来操纵微液滴。 通过在液滴中掺入多个核酸“标签”，并任选地扩增核酸，例如在液滴内，微滴暴露的条件可由核酸编码。 因此，即使暴露于不同条件的液滴混合在一起，每个液滴遇到的条件仍然可以通过例如测序核酸来确定。

    DROPLET CREATION TECHNIQUES
    10.
    发明公开
    DROPLET CREATION TECHNIQUES 审中-公开
    VERFAHREN ZURTRÖPFCHENBILDUNG

    公开(公告)号：EP2493619A1

    公开(公告)日：2012-09-05

    申请号：EP10776469.8

    申请日：2010-10-26

    申请人： President and Fellows of Harvard College

    发明人： WEITZ, David, A. ,  ABATE, Adam, R.

    IPC分类号： B01L3/00 ,  B01F13/00

    CPC分类号： B01L3/502761 ,  B01F3/0807 ,  B01F13/0062 ,  B01F13/0071 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0681 ,  Y10T137/0318 ,  Y10T137/8593

    摘要： The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.

    摘要翻译： 本发明通常涉及用于产生液滴的系统和方法。 液滴可以含有不同的物质，例如用作文库。 在一些情况下，使用诸如流动聚焦技术之类的技术，使用至少一个液滴来产生多个液滴。 在一组实施例中，包含不同种类的多个液滴可以被分开以形成其中包含各种物质的液滴的集合。 根据某些实施方案，液滴的集合可以包含在其中全部含有相同物质的各种液滴亚组。 这种液滴的集合在某些情况下可以用作文库，也可以用于其它目的。