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    SYSTEMS AND METHODS FOR BARCODING NUCLEIC ACIDS
    5.
    发明公开
    SYSTEMS AND METHODS FOR BARCODING NUCLEIC ACIDS 审中-公开
    系统VERFAHREN ZUR BARCODIERUNG VONNUKLEINSÄUREN

    公开(公告)号：EP3134536A1

    公开(公告)日：2017-03-01

    申请号：EP15783629.7

    申请日：2015-04-17

    Applicant: President and Fellows of Harvard College ,  Vilnius University

    Inventor： WEITZ, David, A. ,  KLEIN, Allon, Moshe ,  AKARTUNA, Ilke ,  MAZUTIS, Linas ,  KIRSCHNER, Marc, W.

    IPC: C12P19/34

    Abstract: The present invention generally relates to microfluidics and labeled nucleic acids. For example, certain aspects are generally directed to systems and methods for labeling nucleic acids within microfluidic droplets. In one set of embodiments, the nucleic acids may include "barcodes" or unique sequences that can be used to distinguish nucleic acids in a droplet from those in another droplet, for instance, even after the nucleic acids are pooled together. In some cases, the unique sequences may be incorporated into individual droplets using particles and attached to nucleic acids contained within the droplets (for example, released from lysed cells). In some cases, the barcodes may be used to distinguish tens, hundreds, or even thousands of nucleic acids, e.g., arising from different cells or other sources.

    Abstract translation: 本发明一般涉及微流体和标记的核酸。 例如，某些方面通常涉及用于在微流体液滴内标记核酸的系统和方法。 在一组实施方案中，核酸可以包括可用于将液滴中的核酸与另一个液滴中的核酸区分开的“条形码”或唯一序列，例如甚至在核酸汇集在一起​​之后。 在一些情况下，可以使用颗粒将独特的序列并入单独的液滴中并连接到液滴内的核酸（例如从裂解的细胞中释放）。 在一些情况下，条形码可以用于区分数十，数百甚至数千个核酸，例如由不同细胞或其他来源产生的核酸。

    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION
    6.
    发明公开
    METHODS AND SYSTEMS FOR DROPLET TAGGING AND AMPLIFICATION 审中-公开
    VERFAHREN UND SYSTEME ZURTRÖPFCHENMARKIERUNGUND-EXGRÖSSERUNG

    公开(公告)号：EP3132037A1

    公开(公告)日：2017-02-22

    申请号：EP15780364.4

    申请日：2015-04-17

    Applicant: President and Fellows of Harvard College ,  The General Hospital Corporation d/b/a Massachusetts General Hospital

    Inventor： WEITZ, David, A. ,  IAFRATE, Anthony J. ,  ZHENG, Zongli ,  ZHUANG, Huidan

    IPC: C12N15/10

    CPC classification number: C12N15/1065 ,  C12N15/10 ,  C12N15/1037 ,  C12N15/64 ,  C12N15/66 ,  C12Q1/6844 ,  C12Q2521/101 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: The present invention generally relates to microfluidic devices, including methods and systems for tagging droplets within such devices. In some aspects, microfluidic droplets are manipulated by exposing the droplets (or other discrete entities) to a variety of different conditions. By incorporating into the droplets a plurality of nucleic acid "tags," and optionally amplifying the nucleic acids, e.g., within the droplets, the conditions that a droplet was exposed to may be encoded by the nucleic acids. Thus, even if droplets exposed to different conditions are mixed together, the conditions that each droplet encountered may still be determined, for example, by sequencing the nucleic acids.

    Abstract translation: 本发明一般涉及微流体装置，包括用于在这些装置内标记液滴的方法和系统。 在一些方面，通过将液滴（或其他离散实体）暴露于各种不同的条件来操纵微液滴。 通过在液滴中掺入多个核酸“标签”，并任选地扩增核酸，例如在液滴内，微滴暴露的条件可由核酸编码。 因此，即使暴露于不同条件的液滴混合在一起，每个液滴遇到的条件仍然可以通过例如测序核酸来确定。

    DROPLET CREATION TECHNIQUES
    9.
    发明公开
    DROPLET CREATION TECHNIQUES 审中-公开
    VERFAHREN ZURTRÖPFCHENBILDUNG

    公开(公告)号：EP2493619A1

    公开(公告)日：2012-09-05

    申请号：EP10776469.8

    申请日：2010-10-26

    Applicant: President and Fellows of Harvard College

    Inventor： WEITZ, David, A. ,  ABATE, Adam, R.

    IPC: B01L3/00 ,  B01F13/00

    CPC classification number: B01L3/502761 ,  B01F3/0807 ,  B01F13/0062 ,  B01F13/0071 ,  B01L3/502784 ,  B01L2200/0636 ,  B01L2200/0652 ,  B01L2300/0681 ,  Y10T137/0318 ,  Y10T137/8593

    Abstract: The present invention is generally related to systems and methods for producing droplets. The droplets may contain varying species, e.g., for use as a library. In some cases, at least one droplet is used to create a plurality of droplets, using techniques such as flow-focusing techniques. In one set of embodiments, a plurality of droplets, containing varying species, can be divided to form a collection of droplets containing the various species therein. A collection of droplets, according to certain embodiments, may contain various subpopulations of droplets that all contain the same species therein. Such a collection of droplets may be used as a library in some cases, or may be used for other purposes.

    Abstract translation: 本发明通常涉及用于产生液滴的系统和方法。 液滴可以含有不同的物质，例如用作文库。 在一些情况下，使用诸如流动聚焦技术之类的技术，使用至少一个液滴来产生多个液滴。 在一组实施例中，包含不同种类的多个液滴可以被分开以形成其中包含各种物质的液滴的集合。 根据某些实施方案，液滴的集合可以包含在其中全部含有相同物质的各种液滴亚组。 这种液滴的集合在某些情况下可以用作文库，也可以用于其它目的。

    MULTIPLE EMULSIONS CREATED USING JETTING AND OTHER TECHNIQUES
    10.
    发明公开
    MULTIPLE EMULSIONS CREATED USING JETTING AND OTHER TECHNIQUES 审中-公开
    更多使用波束和其他技术生产的乳剂

    公开(公告)号：EP2473263A2

    公开(公告)日：2012-07-11

    申请号：EP10814401.5

    申请日：2010-09-01

    Applicant: President and Fellows of Harvard College

    Inventor： WEITZ, David, A. ,  THIELE, Julian, W.P. ,  ABATE, Adam, R.

    IPC: B01F5/02 ,  B01F5/26 ,  B01F3/08 ,  B01F15/04

    CPC classification number: B01F3/0807 ,  B01F13/0062 ,  B01F13/0084 ,  B01F2003/0838 ,  B01F2215/045 ,  B01F2215/0459 ,  Y10T137/0318 ,  Y10T137/85938

    Abstract: The present invention generally relates to emulsions, and more particularly, to multiple emulsions. In one aspect, multiple emulsions are formed by urging a fluid into a channel, e.g., by causing the fluid to enter the channel as a “jet.” Side channels can be used to encapsulate the fluid with a surrounding fluid. In some cases, multiple fluids may flow through a channel collinearly before multiple emulsion droplets are formed. The fluidic channels may also, in certain embodiments, include varying degrees of hydrophilicity or hydrophobicity. As examples, the fluidic channel may be relatively hydrophilic upstream of an intersection (or other region within the channel) and relatively hydrophobic downstream of the intersection, or vice versa. In some cases, the average cross-sectional dimension may change, e.g., at an intersection. For instance, the average cross-sectional dimension may increase at the intersection. Surprisingly, a relatively small increase in dimension, in combination with a change in hydrophilicity of the fluidic channel, may delay droplet formation of a stream of collinearly-flowing multiple fluids under certain flow conditions; accordingly, the point at which multiple emulsion droplets are formed can be readily controlled within the fluidic channel. In some cases, the multiple droplet may be formed from the collinear flow of fluids at (or near) a single location within the fluidic channel. In addition, unexpectedly, systems such as those described herein may be used to encapsulate fluids in single or multiple emulsions that are difficult or impossible to encapsulate using other techniques, such as fluids with low surface tension, viscous fluids, or viscoelastic fluids. Other aspects of the invention are generally directed to methods of making and using such systems, kits involving such systems, emulsions created using such systems, or the like.

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