公开(公告)号：EP4060038A1

公开(公告)日：2022-09-21

申请号：EP20887397.6

申请日：2020-11-13

Applicant: Hiroshima University ,  Repertoire Genesis Incorporation

Inventor： ICHINOHE, Tatsuo ,  YAMAMOTO, Takashi ,  SAKUMA, Tetsushi ,  HONJO, Yasuko ,  KAWASE, Takakazu ,  NISHIZAWA, Masatoshi ,  SUZUKI, Ryuji ,  MAGOORI, Kenta ,  SATO, Hiroyuki

IPC: C12N15/09 ,  A61K35/76 ,  A61K38/46 ,  A61K48/00 ,  A61P35/00 ,  A61P37/06 ,  C12N5/0786 ,  C12N5/10 ,  C12N15/12 ,  C12N15/63

Abstract: The present disclosure provides a technique for introducing an antigen receptor-encoding nucleic acid into a definite site in a nucleic acid that is contained in an immune cell of a target organism. In the technique according to the present disclosure, a vector, which contains an antigen receptor-encoding nucleic acid, and a nuclease are introduced into an immune cell. The nuclease cleaves the vector and the genome sequence of the immune cell. The vector can be configured so that, when cleaved with the nuclease, a sequence, which is an antigen receptor-encoding sequence and which is introduced by microhomology-mediated end-joining (MMEJ) at the nuclease cleavage site of the genome sequence of the immune cell, is formed.

公开(公告)号：EP4361267A1

公开(公告)日：2024-05-01

申请号：EP22828547.4

申请日：2022-06-24

Applicant: Repertoire Genesis Incorporation

Inventor： SUZUKI, Ryuji ,  SHIN-I, Tadasu ,  NAKAMURA, Yukio ,  SASAMURA, Takeshi

IPC: C12N15/11 ,  C07K14/705 ,  C07K14/725 ,  C12Q1/68 ,  C40B40/10 ,  G01N33/53

CPC classification number: C07K14/705 ,  C12Q1/68 ,  C12N15/11 ,  C40B40/10 ,  G01N33/53

Abstract: According to the present disclosure, there is provided a method for identifying an epitope sequence.
The present disclosure provides a method for identifying an epitope sequence associated with a specific physiological state of a test subject, the method including the steps of: A) obtaining BCR and/or TCR repertoire data associated with the specific physiological state of the test subject; B) specifying a TREM sequence (repertoire specific TREM sequence) from the BCR and/or TCR repertoire data; C) obtaining a reference TREM sequence associated with the test subject; D) specifying a repertoire-reference common specific TREM sequence from the reference TREM sequence and the repertoire specific TREM sequence; and E) determining, based on the repertoire-reference common specific TREM sequence, whether a peptide sequence including the repertoire-reference common specific TREM sequence is an epitope sequence associated with the specific physiological state of the test subject.

公开(公告)号：EP3702454A1

公开(公告)日：2020-09-02

申请号：EP18866927.9

申请日：2018-10-09

Applicant: Hiroshima University ,  Repertoire Genesis Incorporation

Inventor： ICHINOHE, Tatsuo ,  YAMAMOTO, Takashi ,  SAKUMA, Tetsushi ,  HONJO, Yasuko ,  KAWASE, Takakazu ,  MIYAMA, Takahiko ,  SUZUKI, Ryuji

IPC: C12N15/09 ,  A61K35/17 ,  A61K39/00 ,  A61P37/00 ,  A61P37/06 ,  A61P37/08 ,  C12N5/0783 ,  C12Q1/6869

Abstract: The present disclosure provides a technique for producing regulatory T cells specific to a desired antigen. Regulatory T cells specific to an antigen are produced by a method that includes: a step for identifying a T cell receptor (TCR) clone present in an effector T cell population specific to an antigen in an effector T cell donor; and a step for introducing all or part of the nucleic acid sequence of the TCRα gene and all or part of the nucleic acid sequence of the TCRβ gene included in the clone into a regulatory T cell, said step comprising introducing the TCRα and the TCRβ so as to be expressed in pairs.

公开(公告)号：EP3702453A1

公开(公告)日：2020-09-02

申请号：EP18865880.1

申请日：2018-10-09

Applicant: Hiroshima University ,  Repertoire Genesis Incorporation

Inventor： ICHINOHE, Tatsuo ,  YAMAMOTO, Takashi ,  SAKUMA, Tetsushi ,  HONJO, Yasuko ,  KAWASE, Takakazu ,  MIYAMA, Takahiko ,  SUZUKI, Ryuji

IPC: C12N15/09 ,  A61K35/26 ,  A61P35/00 ,  C07K19/00 ,  C12N5/0783 ,  C12N15/62

Abstract: The present disclosure provides a technique whereby the influence of an endogenous TCR is eliminated in TCR gene transfer. A TCR gene is edited using a genome editing enzyme, said genome editing enzyme having one characteristic that amino acids at two specific positions in DNA-binding modules contained in a DNA-binding domain thereof show repeating patterns which differ from one module to another among the four DNA-binding modules. Thus, a lowering in the expression efficiency of the transferred TCR caused by mispairing with an endogenous TCR and the occurrence of a self-reactive TCR are avoided.

公开(公告)号：EP3486321A1

公开(公告)日：2019-05-22

申请号：EP17815521.4

申请日：2017-06-23

Applicant: Riken ,  Repertoire Genesis Incorporation

Inventor： SHIROGUCHI, Katsuyuki ,  SUZUKI, Ryuji ,  MATSUTANI, Takaji ,  KITAURA, Kazutaka

IPC: C12N15/09 ,  C12Q1/68

Abstract: The present invention provides a method for analyzing the variable region repertoire of the T-cell receptors (TCR) or the B-cell receptors (BCR) of a subject, said method including: (1) a step for providing a nucleic acid sample that is amplified from RNA obtained from the subject using one-step reverse transcription template-switching PCR and that includes the nucleic acid sequences of a plurality of types of T-cell receptors (TCR) or B-cell receptors (BCR); (2) a step for determining the nucleic acid sequences included in the nucleic acid sample; and (3) a step for calculating the frequencies of occurrence of individual genes or combinations thereof on the basis of the determined nucleic acid sequences and deriving the TCR or BCR repertoire of the subject.

公开(公告)号：EP3091074A1

公开(公告)日：2016-11-09

申请号：EP14864397.6

申请日：2014-11-20

Applicant: Repertoire Genesis Incorporation

Inventor： SUZUKI, Ryuji ,  SHINI, Tadasu

IPC: C12N15/09 ,  C12Q1/68 ,  G06F19/22

CPC classification number: C12Q1/6881 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/172 ,  G06F19/22

Abstract: The repertoire of the variable region of T cell receptors (TCR) or B cell receptors (BCR) is quantitatively analyzed using non-biased gene sequence analysis. The present invention provides the following: a method for quantitatively analyzing the repertoire of the variable region of the T cell receptors (TCR) or B cell receptors (BCR) of a subject by using a database, wherein the method includes (1) a step for providing a nucleic acid sample containing the nucleic acid sequence of T cell receptors (TCR) or B cell receptors (BCR) amplified in a non-biased manner from the subject; (2) a step for determining the nucleic acid sequence contained in the nucleic acid sample; and (3) a step for calculating the frequency of appearance of each gene or combination thereof on the basis of the determined nucleic acid sequence and deriving the TCR or BCR repertoire of the subject.

Abstract translation: 使用非偏向基因序列分析定量分析T细胞受体（TCR）或B细胞受体（BCR）可变区的所有组成成分。 本发明提供以下方法：通过使用数据库对受试者的T细胞受体（TCR）或B细胞受体（BCR）的可变区的定量分析的方法，其中，所述方法包括（1）步骤 用于提供含有从受试者以非偏倚的方式扩增的T细胞受体（TCR）或B细胞受体（BCR）的核酸序列的核酸样品; （2）确定核酸样品中所含的核酸序列的步骤; 和（3）基于确定的核酸序列计算每种基因或其组合的出现频率并导出受试者的TCR或BCR谱的步骤。