公开(公告)号：EP1490471B1

公开(公告)日：2016-06-15

申请号：EP03718019.7

申请日：2003-03-25

申请人： THE GENERAL HOSPITAL CORPORATION

发明人： VACANTI, Joseph, P. ,  SHIN, Young-Moon, Michael ,  OGILVIE, Jennifer ,  SEVY, Alexander ,  MAEMURA, Tomoyuki ,  ISHI, Osamu ,  KAAZEMPUR-MOFRAD, Mohammad Reza ,  BORENSTEIN, Jeffrey T. ,  KING, Kevin R. ,  WANG, Chiao-Chun ,  WEINBERG, Eli, The Charles Stark Draper Lab. Inc.

IPC分类号： C12N5/07 ,  A61L27/36 ,  A61L27/38 ,  C12M3/00 ,  C12N5/00 ,  C12N5/071 ,  C12M1/12 ,  C12M1/00

CPC分类号： A61F2/062 ,  A61F2240/004 ,  A61F2240/005 ,  A61L27/3604 ,  A61L27/3804 ,  A61L27/3808 ,  A61L27/3891 ,  A61L27/3895 ,  A61L27/58 ,  A61L2300/64 ,  B33Y80/00 ,  C12M21/08 ,  C12M23/06 ,  C12M23/30 ,  C12M25/02 ,  C12N5/0062 ,  C12N5/0697 ,  C12N2533/12 ,  C12N2533/30 ,  Y10T29/49826

摘要： Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.