公开(公告)号：EP1416947A1

公开(公告)日：2004-05-12

申请号：EP02750240.0

申请日：2002-07-23

申请人： ZymoGenetics, Inc.

发明人： ZAMOST, Bruce, L.

IPC分类号： A61K35/72 ,  A61K38/00 ,  A61K38/16 ,  A61K38/36 ,  A61K38/37 ,  C07K1/00 ,  C12N1/00 ,  C12N1/14 ,  C12N1/16 ,  C12N1/18 ,  C12P1/00 ,  C12P1/02 ,  C12P21/00

CPC分类号： C12N1/18 ,  C07K14/755

摘要： A yeast fermentation medium that does not contain yeast cell extract but is comprised of a mixture of oleic acid, lactic acid and palmitic acid. Using this fermentation medium yields of factor XIII were increased 250 %.

公开(公告)号：EP1931704A1

公开(公告)日：2008-06-18

申请号：EP06816409.4

申请日：2006-10-04

申请人： Zymogenetics, Inc.

发明人： ZAMOST, Bruce, L. ,  LEE, Geoffrey, F. ,  DEDINSKY, Robert, M.

IPC分类号： C07K14/54

CPC分类号： C12P21/02 ,  C07K14/54

摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-29 are described. The vectors utilize the IL-29 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. Also included are methods of producing, purifying and pegylating an IL-29 polypeptide.

公开(公告)号：EP1573006B1

公开(公告)日：2009-11-11

申请号：EP03810068.1

申请日：2003-12-12

申请人： ZymoGenetics, Inc.

发明人： CHANG, Chung ,  ZAMOST, Bruce, L. ,  COVERT, Douglas, C. ,  LIU, Hong, Y. ,  DE JONGH, Karen, S. ,  MEYER, Jeffrey, D. ,  HOLDERMAN, Susan, D.

IPC分类号： C12N15/00

CPC分类号： C07K14/54

公开(公告)号：EP1573006A2

公开(公告)日：2005-09-14

申请号：EP03810068.1

申请日：2003-12-12

申请人： ZymoGenetics, Inc.

发明人： CHANG, Chung ,  ZAMOST, Bruce, L. ,  COVERT, Douglas, C. ,  LIU, Hong, Y. ,  DE JONGH, Karen, S. ,  MEYER, Jeffrey, D. ,  HOLDERMAN, Susan, D.

IPC分类号： C12N15/00

CPC分类号： C07K14/54

摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. . Using the expression vectors, the IL-21 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains transformed with an IL-21 expression vector.