公开(公告)号：EP1966371A1

公开(公告)日：2008-09-10

申请号：EP06846013.8

申请日：2006-12-21

申请人： Zymogenetics, Inc.

发明人： MALLET, Robert, W. ,  STENLAND, Christopher, J. ,  BOONE, Jonathon, C. ,  FORSTROM, John, W. ,  DE JONGH, Karen, S.

IPC分类号： C12N9/00

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.

公开(公告)号：EP1966371B1

公开(公告)日：2011-08-24

申请号：EP06846013.8

申请日：2006-12-21

申请人： Zymogenetics, Inc.

发明人： MALLET, Robert, W. ,  STENLAND, Christopher, J. ,  BOONE, Jonathon, C. ,  FORSTROM, John, W. ,  DE JONGH, Karen, S.

IPC分类号： C12N9/00

CPC分类号： C12N9/6429 ,  C12Y304/21005

摘要： Methods for converting prethrombin-1 to thrombin are disclosed. An aqueous solution of prethrombin-1 is applied to oscutarin-C immobilized on a solid support so as to provide from 500 mg to 4000 mg of prethrombin-1 per mL of the solid support and a contact time between the prethrombin-1 and the oscutarin-C of from 1.8 to 3.5 minutes. The resulting active thrombin may be captured on an ion exchange chromatography medium or an affinity chromatography medium.

公开(公告)号：EP1573006B1

公开(公告)日：2009-11-11

申请号：EP03810068.1

申请日：2003-12-12

申请人： ZymoGenetics, Inc.

发明人： CHANG, Chung ,  ZAMOST, Bruce, L. ,  COVERT, Douglas, C. ,  LIU, Hong, Y. ,  DE JONGH, Karen, S. ,  MEYER, Jeffrey, D. ,  HOLDERMAN, Susan, D.

IPC分类号： C12N15/00

CPC分类号： C07K14/54

公开(公告)号：EP1573006A2

公开(公告)日：2005-09-14

申请号：EP03810068.1

申请日：2003-12-12

申请人： ZymoGenetics, Inc.

发明人： CHANG, Chung ,  ZAMOST, Bruce, L. ,  COVERT, Douglas, C. ,  LIU, Hong, Y. ,  DE JONGH, Karen, S. ,  MEYER, Jeffrey, D. ,  HOLDERMAN, Susan, D.

IPC分类号： C12N15/00

CPC分类号： C07K14/54

摘要： The expression vectors and methods using an E. coli expression system for the large scale production of IL-21 are described. The vectors utilize the IL-21 coding sequence with specific changes in nucleotides in order to optimize codons and mRNA secondary structure for translation in E. coli. . Using the expression vectors, the IL-21 gene was produced in E. coli to a level of greater than 1 g/L in fed batch fermentation. Also included are OmpT deficient E. coli strains transformed with an IL-21 expression vector.