公开(公告)号：EP1564554A1

公开(公告)日：2005-08-17

申请号：EP04075439.2

申请日：2004-02-12

申请人： PEPSCAN SYSTEMS B.V. ,  Universiteit Utrecht Holding B.V.

发明人： Akresteijn, Gerardus Josephus Adrianus ,  Hensen, Everard Johannes ,  Scibelli, Antonio ,  van der Most, Robbert Gerrit ,  Meloen, Robert Hans ,  Turkstra, Jouwert Anne

IPC分类号： G01N33/50

CPC分类号： G01N33/56972 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/54393

摘要： The present invention discloses an affinity-binding assay comprising a particle having at least four copies of a target molecule and at least two binding molecules specific for said target molecule, wherein a first of said binding molecules is associated with a first label and a second of said binding molecules is associated with a second label, wherein a particle having said first label and a particle having said second label are distinguishable from a particle having both said first and said second label, wherein said first and said second binding molecule each comprise at least two binding regions specific for said target molecule.
The present invention also discloses a composition comprising a first binding molecule associated with a first label and a second binding molecule associated with a second label, wherein the signal obtained from said first label and said second label is distinguishable from the combined signal of said first and second label, characterised in that said first and said second binding molecule each comprise at least two binding regions specific for essentially the same target molecule, preferably for essentially the same epitope on said target molecule and a method for selecting a synthetic antigen from a collection of at least three antigens comprising using the disclosed composition and method.
The invention further discloses an antigen obtainable by above described method and capable of inducing an early immune response, a kit of parts to perform said method, the use of antigens selected by said methods for use as a vaccine, and the use of antibodies and antigens as a medicament.

摘要翻译： 本发明公开了一种亲和力结合测定法，其包含具有至少四个拷贝的靶分子和至少两个对所述靶分子特异性的结合分子的颗粒，其中第一个所述结合分子与第一个标记相关联，第二个 所述结合分子与第二标记相关联，其中具有所述第一标记的颗粒和具有所述第二标记的颗粒与具有所述第一和所述第二标记两者的颗粒可区分，其中所述第一和所述第二结合分子各自包含至少 对于所述靶分子具有特异性的两个结合区。 本发明还公开了一种组合物，其包含与第一标记相关联的第一结合分子和与第二标记相关联的第二结合分子，其中从所述第一标记和所述第二标记获得的信号与组合信号区分开 的所述第一和第二标记，其特征在于所述第一和所述第二结合分子各自包含至少两个对基本相同的靶分子特异性的结合区，优选用于所述靶分子上基本相同的表位，以及选择合成抗原的方法 包括使用所公开的组合物和方法的至少三种抗原的集合。 本发明还公开了可通过上述方法获得的能够诱导早期免疫应答的抗原，用于实施所述方法的试剂盒，所述方法选择的抗原用作疫苗，以及使用 的抗体和抗原作为药物。

公开(公告)号：EP1471362A1

公开(公告)日：2004-10-27

申请号：EP03076199.3

申请日：2003-04-24

申请人： Universiteit Utrecht Holding B.V. ,  UMC Utrecht Holding B.V.

发明人： Seppenwoolde, Jan Hendrik ,  Bakker, Christianus Johannes Gerardus

IPC分类号： G01R33/28

CPC分类号： G01R33/286

摘要： Method for magnetic resonance imaging (MRI) of at least one specific element, wherein signal of background tissue represented in an MR image is at least partially dephased by applying a gradient imbalance or additional gradient, wherein signal around said element is accordingly conserved, resulting in a selective depiction of said element.

摘要翻译： 通过应用梯度不平衡或附加梯度，在MR图像中表示的背景组织的信号被部分解相。 元件周围的信号相应地被保存，导致元素的选择性描绘。 还包括以下独立权利要求：（a）用于被动跟踪包括抗磁性，顺磁性或铁磁性元件的装置的方法; （b）使用MRI中的去相信号，特别是器件跟踪，用于MRI的图像中的特定元素的正对比的选择性描绘; （c）增强MRI装置上MR图像对比度的方法; 和（d）MRI装置。

公开(公告)号：EP1447412A1

公开(公告)日：2004-08-18

申请号：EP03075460.0

申请日：2003-02-17

申请人： Universiteit Utrecht Holding B.V.

发明人： Braakman, Lina Jantina ,  van Anken, Eelco

IPC分类号： C07K14/47 ,  G01N27/447

CPC分类号： C07K14/47 ,  G01N33/5076 ,  G01N33/6803

摘要： The invention provides a novel method of analysing the proteome of a cell. The method allows the correlation of expression of proteins with the activity of compartments in the cell and the association of thus far unidentified proteins with(in) such compartments. This gives the artisan a powerful tool in the elucidation of the function of proteins thus identified. According to this method a new family of proteins was identified and the function thereof determined and confirmed by experiments. The family termed smERp belongs to the oxidoreductase proteins, but is distinct therefrom in their activity and internal structure. The invention further identifies several other proteins and their function, together with methods wherein the identified proteins can be manipulated to obtain, or gain further functionality. The invention also provides methods wherein proteins identified in the present invention are used for the production of proteins of interest.

摘要翻译： 本发明提供了一种分析细胞蛋白质组的新方法。 该方法允许蛋白质的表达与细胞中室的活性和迄今未鉴别的蛋白质与（在）这种隔室中的相互作用的相关性。 这给了工匠一个强大的工具，用于阐明所鉴定的蛋白质的功能。 根据该方法，鉴定出新的蛋白质家族，并通过实验确定并证实其功能。 称为smERP的家族属于氧化还原酶蛋白，但与其活性和内部结构不同。 本发明进一步鉴定了其他蛋白质及其功能，以及可以操作鉴定的蛋白质以获得或获得更多功能的方法。 本发明还提供了将本发明中鉴定的蛋白质用于生产感兴趣的蛋白质的方法。

公开(公告)号：EP1275731A1

公开(公告)日：2003-01-15

申请号：EP01202638.1

申请日：2001-07-09

申请人： Universiteit Utrecht Holding B.V.

发明人： Breukink, Eefjan

IPC分类号： C12P21/00 ,  G01N33/92

CPC分类号： C12P21/005

摘要： The present invention relates to a method for preparing Lipid II, which method comprises the steps of preparing a mixture of bacterial membranes, uridine diphosphate N-acetylmuramyl peptide (UDP-MurNAc), uridine diphosphate N-acetylglucosamine (GlcNAc), a chain of n prenyl residues, wherein n is at least 2, and optionally a detergent in a buffer; reacting the mixture thus obtained to prepare an n-Lipid II containing mixture, wherein n represents the amount of prenyl residues in the Lipid II molecule and wherein n is at least 2; and optionally further purifying the n-Lipid II containing mixture. Preferably n is selected from the range 2-25. Instead of bacterial membranes the reaction mixture may comprise isolated enzymes, such as MraY (undecaprenyl phosphate phospho-N-acetylmuramoyl-pentapeptide transferase).

摘要翻译： 本发明涉及一种制备Lipid II的方法，该方法包括以下步骤：制备细菌膜，尿苷二磷酸N-乙酰木瓜霉素（UDP-MurNAc），尿苷二磷酸N-乙酰葡糖胺（GlcNAc），n链 异戊烯基残基，其中n至少为2，以及任选的缓冲剂中的洗涤剂; 使由此获得的混合物反应以制备含有脂质II的混合物，其中n表示脂质II分子中异戊烯基残基的量，并且其中n至少为2; 并任选地进一步纯化含有n-Lipid II的混合物。 优选地，n从2-25的范围选择。 代替细菌膜，反应混合物可以包含分离的酶，例如MraY（磷酸-N-十二烷基酯 - 磷酸-N-乙酰基甲酰基 - 五肽转移酶）。