公开(公告)号：EP0572737A3

公开(公告)日：1994-11-23

申请号：EP92311006.8

申请日：1992-12-02

Applicant: THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY

Inventor： Saito, Atsushi ,  Sinagawa, Hideo ,  Nakata, Atsuo

IPC: C12N15/49 ,  C12N15/62 ,  A61K39/21 ,  C07K13/00 ,  G01N33/53

CPC classification number: C07K14/005 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/40 ,  C12N2740/16122 ,  C12N2740/16222 ,  C12N2740/16322 ,  Y10S435/974

Abstract: Disclosed is a substantially pure HIV antigen comprising a Gag-Env fusion protein consisting of a Gag peptide fused at its C-terminus to an Env peptide, wherein the Gag peptide comprises a contiguous sequence of at least ten amino acids of the amino acid sequence represented by Gag (308-437) and the Env peptide comprises a contiguous sequence of at least a part of the amino acid sequence represented by Env (512-699), the part containing at least one epitope which is reactive to an HIV antibody. The gag - env fusion DNA corresponding to the HIV antigen of the present invention allows the production of the desired high antigenicity HIV antigen in high yield. Therefore, the HIV antigen of the present invention can be advantageously used as an active component for a diagnostic reagent, a vaccine, an antibody preparation and a therapeutic reagent for AIDS. Also disclosed is a substantially pure HIV antigen comprising a Gag protein coded for by the entire gag gene.

公开(公告)号：EP0464287A1

公开(公告)日：1992-01-08

申请号：EP90314371.7

申请日：1990-12-28

Applicant: The Research Foundation for Microbial Diseases of Osaka University

Inventor： Okayama, Hiroto ,  Fuke, Isao ,  Mori, Chisato ,  Takamizawa, Akihisa ,  Yoshida, Iwao

IPC: C12N15/40 ,  C12Q1/70 ,  A61K39/29 ,  C07K15/00 ,  G01N33/569

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N2770/24222

Abstract: Disclosed is an isolated non-A, non-B hepatitis virus genomic cDNA covering the entire region of the virus gene nucleotide sequence from the 1 st to 9416th nucleotides shown in Fig. 2(1) through Fig. 2(16) hereof, wherein the coding region is from the 333rd to 9362nd nucleotides, and the 5'- and 3'-noncoding sequences contain 332 nucleotides and 54 nucleotides, respectively. Part or whole of the cDNA and an antigen polypeptide as an expression product thereof are useful as a diagnostic agent for non-A, non-B hepatitis. The antigen polypeptide is also useful as an active ingredient for a non-A, non-B hepatitis virus vaccine.

Abstract translation: 公开了一种分离的非A非乙型肝炎病毒基因组cDNA，其覆盖图1所示的第1至第9416位核苷酸的病毒基因核苷酸序列的整个区域。 2（1）至图 2（16），其中编码区为333〜9362个核苷酸，5'-和3'-非编码序列分别含有332个核苷酸和54个核苷酸。 作为其表达产物的部分或全部cDNA和抗原多肽可用作非A非乙型肝炎的诊断剂。 抗原多肽也可用作非A非乙型肝炎病毒疫苗的活性成分。

公开(公告)号：EP0372904A3

公开(公告)日：1991-09-25

申请号：EP89312662.3

申请日：1989-12-05

Applicant: The Research Foundation for Microbial Diseases of Osaka University

Inventor： Saito, Atsusi, ,  Shinagawa, Hideo, ,  Nakata, Atsuo,

IPC: C12N15/57 ,  C12N15/54 ,  C12N15/55 ,  C12N15/48 ,  C12N9/50 ,  C12N9/10 ,  C12N9/16

CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N9/1276 ,  C12N9/22 ,  C12N9/506 ,  C12N2740/14022 ,  C12N2740/16122

Abstract: Disclosed is a method for producing retroviral proteins which are protease, reverse transcriptase, endonuclease and Gag proteins. The method is characterized by the consecutive accomplishment of expression and processing of retroviral genes and these products under the stepwise cultivation of hosts transformed with a vector constructed to carry retroviral gene fragments comprising at least a protease gene and one or more of the other genes coding for retroviral proteins. The retroviral proteins of this invention are used as specific reagens for the diagnosis of retroviral disease, e.g., AIDS, malignant tumors and so forth, also may be used as the basis for research and development of antiviral agents and a vaccine against the above infectious diseases, and for genetic engineering.

公开(公告)号：EP0296765A3

公开(公告)日：1990-02-07

申请号：EP88305530.3

申请日：1988-06-17

Applicant: TEIJIN LIMITED ,  National Institute of Health ,  The Research Foundation for Microbial Diseases of Osaka University

Inventor： Sato, Yuji ,  Sato, Hiroko ,  Yoshida, Iwao ,  Imaizumi, Atsushi

IPC: A61K39/10 ,  C12N1/10 ,  C07K15/00

CPC classification number: C07K14/235 ,  A61K39/00

Abstract: A Bordetella pertussis variant which produces a pertussis toxin mutant protein partially devoid of subunits. When the variants of the present invention are cultured, a pertussis toxin mutant protein partially devoid of subunits, particularly at least subunit S1, can be harvested from the culture. The thus-obtained pertussis toxin mutant protein partially devoid of subunits can be applied to the preparation of a per­tussis vaccine by a conventional method.

Abstract translation: 产生百日咳毒素突变体蛋白质的百日咳博德特氏菌变体部分缺乏亚基。 当培养本发明的变体时，可以从培养物中收获部分缺乏亚基，特别是至少亚基S1的百日咳毒素突变蛋白。 由此获得的百日咳毒素突变蛋白部分缺乏亚基可以通过常规方法用于制备百日咳疫苗。