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公开(公告)号：EP1857540B1
公开(公告)日：2010-07-07
申请号：EP07108446.1
申请日：2007-05-18
发明人： Crespi, Charles L. , Chen, Xiaoxi
IPC分类号： C12M3/06
CPC分类号： C12M23/20 , C12M25/04 , C12M33/06 , Y10T436/25375
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公开(公告)号：EP2079827A2
公开(公告)日：2009-07-22
申请号：EP07867228.4
申请日：2007-10-12
IPC分类号： C12M1/20 , G01N33/543 , B01J19/00
CPC分类号： G01N33/5067 , B01J19/0046 , B01J2219/00317 , B01J2219/00587 , B01J2219/0898 , B01L3/5025 , B01L2200/0647 , B01L2200/12 , B01L2300/0636 , C12M23/12 , C12M23/20 , C12M25/04 , C12N5/0671 , C12N2502/14 , C12N2535/10 , G01N33/5008 , G01N33/54353 , G01N33/5436
摘要： The present invention is drawn to the generation of micropatterns of biomolecules and cells on standard laboratory materials through selective ablation of a physisorbed biomolecule with oxygen plasma. In certain embodiments, oxygen plasma is able to ablate selectively physisorbed layers of biomolecules (e.g., type-I collagen, fibronectin, laminin, and Matrigel) along complex non-linear paths which are difficult or impossible to pattern using alternative methods. In addition, certain embodiments of the present invention relate to the micropatterning of multiple cell types on curved surfaces, multiwell plates, and flat bottom flasks. The invention also features kits for use with the subject methods.
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公开(公告)号：EP2044192A2
公开(公告)日：2009-04-08
申请号：EP07799408.5
申请日：2007-07-09
申请人： University of Miami
摘要： Provided is a cell culture apparatus for culturing cells, that provides enhanced oxygen delivery and supply to cells without the need for stirring or sparging, Oxygen diffusions occurs on both sides of the culture vessel, top and bottom, A gas-permeable membrane that includes perfluorocarbons in its composition allows for the rapid, enhanced and uniform transfer of oxygen between the environment of cells or tissues contained in the cell culture container apparatus and the atmosphere of the incubator in which, the cell culture apparatus is incubated.
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公开(公告)号：EP1974008A2
公开(公告)日：2008-10-01
申请号：EP07718107.1
申请日：2007-01-18
申请人： KCI Licensing, Inc.
发明人： WILKES, Robert, Peyton , MCNULTY, Amy, Kathleen , KIESWETTER, Kristine , FEELEY, Teri, D. , SCHMIDT, Marisa
IPC分类号： C12M1/12
摘要： A method and system for culturing cells, having a substantially airtight enclosure configured to culture cells. The method and system also have a first conduit configured to provide a reduced pressure to the substantially airtight enclosure and a second conduit configured to provide a culture media to the substantially airtight enclosure.
摘要翻译： 一种用于培养细胞的方法和系统，具有基本气密的外壳，其被配置为培养细胞。 该方法和系统还具有第一导管和第二导管，该第一导管被配置成向该基本气密外壳提供减压，该第二导管被配置为向该基本气密外壳提供培养基。
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105.发明公开
公开(公告)号：EP1733793A3
公开(公告)日：2008-07-09
申请号：EP06253111.6
申请日：2006-06-15
CPC分类号： B01L3/50255 , B01L2200/026 , B01L2300/0829 , C12M25/04
摘要： Multiwell filter comprising a filter plate having a plurality of wells, each including a support. Each well has a corresponding adjacent access port providing access to the corresponding feeding/receiver plate well, e.g. via a pipette. When the filter plate is positioned over a feeding/receiver plate having a plurality of wells, each well of the feeding/receiving plate aligns with and communicates with a corresponding filter plate well. Each access port provides access to a respective well in the feeding/receiver plate, and the wells in the filter plate are not centrally located with respect to the feeding/receiver plate wells, but are shifted in the x and y directions relative to the receiver wells in an amount effective for reducing or eliminating capillary holdup in the gap between the filter wells and the feeding/receiver wells. The wells in the feeding/receiver plate preferably are formed in a U-shaped bottom below each access port.
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公开(公告)号：EP1853695A2
公开(公告)日：2007-11-14
申请号：EP06820794.3
申请日：2006-01-26
申请人： BASF Beauty Care Solutions France SAS , Centre National de la Recherche Scientifique , University Claude Bernard Lyon I
发明人： PERRIER, Eric , DAMOUR, Odile , ANDRE, Valerie
IPC分类号： C12M1/22
摘要： The invention relates to a cell culture device. This cell culture device (100), intended for receiving cells with their culture medium, is characterised in that it contains at least one cell-supporting layer of porous material, this layer being obtained by dehydration of an aqueous gel which is poured directly either into said well bottom (110-111), or into the bottom of a cradle or insert (150) of a size which is adapted for being insertable into the volume of the well. The invention enables a culture to be made which can be automated.
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公开(公告)号：EP0954561A1
公开(公告)日：1999-11-10
申请号：EP98900896.0
申请日：1998-01-14
申请人： Smith & Nephew plc
发明人： BLOTT, Patrick, Lewis , POOLE, Dawn
IPC分类号： C12M1
摘要： A cell culture substrate device comprising a frame and a cell culture substrate material is disclosed. The frame is adapted to releasably secure the substrate material in preferably a flat configuration for the attachment of cells. The substrate material may further comprise a support layer. The device may be placed within a container, e.g. Petri-dish.
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公开(公告)号：EP0866122A3
公开(公告)日：1999-06-30
申请号：EP98302074.4
申请日：1998-03-19
摘要： A multiwell tissue culture assembly for culturing cells comprising a plate and a lid. The plate includes a plurality of wells for receiving liquids therein and the lid includes means for varying the gas diffusion into and out of the assembly and for substantially minimizing biological contamination of the cells being cultured. The means for varying the gas diffusion is a gas permeable membrane provided in an opening in the lid and a removable thin impermeable film label is provided for selectively occluding passage of gases through the gas permeable membrane. The means for minimizing biological contamination of the cells being cultured is a sealing material located in the lid and means for removably securing the lid and the plate together whereby the sealing material is compressed between the lid and the plate.
摘要翻译： 用于培养包含板和盖的细胞的多孔组织培养组件。 该板包括用于在其中接收液体的多个孔，并且该盖包括用于改变气体扩散进入和离开该组件的装置，并且用于基本上最小化正在培养的细胞的生物污染。 用于改变气体扩散的装置是在盖中的开口中提供的气体可渗透膜，并且提供可移除的薄的不可渗透的膜标签以选择性地阻止气体通过气体可渗透膜。 用于最小化培养的细胞的生物污染的装置是位于盖子中的密封材料和用于将盖子和板可拆卸地固定在一起的装置，由此密封材料在盖子和板之间被压缩。
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公开(公告)号：EP0842262A1
公开(公告)日：1998-05-20
申请号：EP96926231.0
申请日：1996-08-02
发明人： MATHUS, Gregory
CPC分类号： C12M25/04 , Y10S435/809
摘要： A device and method for growing tissue or cells on both sides of a membrane includes a cylindrical body which may be stood on either end in a culture well. A frame is slidably mounted in the body and carries a membrane, on both sides of which tissue or cells are to be grown. A tissue or cell sample is placed on the upper surface of the membrane and media is supplied to the well on the outside of the body. Passages at the bottom of the body allow the media to flow to the membrane and nurture the sample to cause its growth. When the first monolayer is grown, the body is inverted and the frame is pushed to the other end of the body, preferably by a jig supplied for that purpose. A second cell sample is deposited on the other side of the membrane, again on top of the membrane and it is nurtured by media supplied primarily from the reservoir on top and secondarily through the membrane.
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公开(公告)号：EP0800571A2
公开(公告)日：1997-10-15
申请号：EP96903480.0
申请日：1996-01-02
发明人： RISING, Donald, B.
CPC分类号： B01L3/50853 , B01L3/50255 , B01L2200/0684 , B01L2300/0829 , B01L2400/0688 , C12M23/04 , C12M23/12 , C12M25/04 , Y10S435/809 , Y10S436/809
摘要： A device for conducting biological analysis, such as cell culture, is disclosed having improved contamination prevention features. In a preferred embodiment, a microwell membrane plate for cell analysis is adapted to be inserted into a shallow rectangular tray with elevated side walls which form a single large reservoir for holding an appropriate liquid used in cell growth and analysis. The side surfaces of the tray include a raised ridge extending about the entire periphery of the tray. A series of uniform-height steps formed on the ridge serve as support for the microwell membrane plate when inserted into the tray and also create a narrow capillary gap between the plate and the tray. A ledge extending laterally from the base of the ridge forms a second, wider gap when the microwell membrane plate is inserted into the tray. The height of the first capillary gap is less than the height of the second gap formed within the zone of space extending to the exterior of the device, thus when the tray and plate are tilted, as when jostled during handling, liquid from the reservoir will preferentially remain in the space formed by the first capillary gap. Liquid which does spill into the second gap will run back into the reservoir when the device is leveled.
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