公开(公告)号：EP3414319B1

公开(公告)日：2024-07-24

申请号：EP17750748.0

申请日：2017-02-09

IPC分类号： C12N5/071 ,  C12N5/02 ,  C12N5/00 ,  C12M1/00 ,  C12M3/00 ,  C12M1/22 ,  C12M3/06

CPC分类号： C12M21/08 ,  C12M23/10 ,  C12M23/16 ,  C12N5/0671 ,  C12N5/0672 ,  C12N5/0697 ,  C12N5/0645

公开(公告)号：EP3431586B1

公开(公告)日：2024-07-10

申请号：EP17759829.9

申请日：2017-02-24

IPC分类号： C12N5/10

CPC分类号： A61K31/573 ,  C12N5/0696 ,  C12N2506/4520130101 ,  C12N5/067 ,  C12N2533/5420130101 ,  C12N5/0671 ,  C12N2501/1220130101 ,  C12N2501/11520130101 ,  C12N2501/15520130101

公开(公告)号：EP3368657A1

公开(公告)日：2018-09-05

申请号：EP16801292.0

申请日：2016-10-29

申请人： Biolamina AB ,  The University of Edinburgh

发明人： HAGBARD, Louise, Kristina ,  ERICCSON, Carl, Gunnar Jesper ,  CAMERON, Katherine, Rachel ,  HAY, David, Colin ,  FORBES, Stuart, John ,  RASHIDI, Hassan

IPC分类号： C12N5/071

CPC分类号： C12N5/0671 ,  C12N5/067 ,  C12N2500/25 ,  C12N2500/38 ,  C12N2500/62 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/16 ,  C12N2501/237 ,  C12N2501/39 ,  C12N2501/415 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2513/00 ,  C12N2533/40 ,  C12N2533/52 ,  C12N2533/76 ,  C12N2533/90

摘要： Methods for producing hepatocytes from pluripotent human stem cells are disclosed herein. The stem cells are plated on a cell culture substrate comprising two laminins. The stem cells are then exposed to different cell culture mediums to induce differentiation. The resulting hepatocytes have higher metabolic capacity compared to hepatocytes cultured on different substrates.

公开(公告)号：EP2313487B1

公开(公告)日：2018-04-04

申请号：EP09790529.3

申请日：2009-07-16

申请人： CHILDREN'S MEDICAL CENTER CORPORATION

发明人： INGBER, Donald, E. ,  HUH, Dongeun

IPC分类号： C12M3/06 ,  B01L3/00 ,  C12N5/07

CPC分类号： C12M23/16 ,  B01L3/5027 ,  B01L3/50273 ,  B01L2200/0663 ,  B01L2300/0854 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/163 ,  B01L2400/0472 ,  B01L2400/0481 ,  B01L2400/0487 ,  C12M21/08 ,  C12M23/58 ,  C12M25/02 ,  C12M29/10 ,  C12N5/0606 ,  C12N5/061 ,  C12N5/0623 ,  C12N5/0647 ,  C12N5/0654 ,  C12N5/0662 ,  C12N5/0671 ,  C12N5/0688 ,  C12N5/0696 ,  C12N5/0697 ,  G01N33/5005 ,  G01N33/5088 ,  G01N33/5091

摘要： System and method includes a body having a central microchannel separated by one or more porous membranes. The membranes are configured to divide the central microchannel into a two or more parallel central microchannels, wherein one or more first fluids are applied through the first central microchannel and one or more second fluids are applied through the second or more central microchannels. The surfaces of each porous membrane can be coated with cell adhesive molecules to support the attachment of cells and promote their organization into tissues on the upper and lower surface of the membrane. The pores may be large enough to only permit exchange of gases and small chemicals, or to permit migration and transchannel passage of large proteins and whole living cells. Fluid pressure, flow and channel geometry also may be varied to apply a desired mechanical force to one or both tissue layers.

公开(公告)号：EP2588027B8

公开(公告)日：2017-07-26

申请号：EP11801504.9

申请日：2011-07-01

申请人： The University of North Carolina at Chapel Hill ,  Roach, Marsha Lynn ,  Malavarca, Richard Harold

发明人： ROACH, Marsha Lynn ,  MALAVARCA, Richard Harold ,  WANG, Yunfang ,  REID, Lola Cynthia McAdams

IPC分类号： C12N5/00 ,  C12N5/09 ,  C12N5/071 ,  A61L27/36

CPC分类号： C12N5/0068 ,  A61L27/24 ,  A61L27/3604 ,  A61L27/3683 ,  A61L27/3691 ,  A61L2430/28 ,  C12N5/067 ,  C12N5/0671 ,  C12N5/0693 ,  C12N7/00 ,  C12N2500/25 ,  C12N2500/36 ,  C12N2501/20 ,  C12N2501/998 ,  C12N2506/14 ,  C12N2533/54 ,  C12N2533/90

公开(公告)号：EP2443230B1

公开(公告)日：2017-07-26

申请号：EP10730123.6

申请日：2010-06-18

申请人： Takara Bio Europe AB

发明人： STACHELSCHEID, Harald ,  ZEILINGER, Katrin ,  JENSEN, Janne ,  URBANIAK, Thomas

IPC分类号： C12M1/12 ,  C12N5/071

CPC分类号： C12N5/067 ,  C12M25/10 ,  C12N5/0671 ,  C12N2500/25 ,  C12N2500/34 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/115 ,  C12N2501/12 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/23 ,  C12N2501/39 ,  C12N2501/70 ,  C12N2506/02

公开(公告)号：EP3059308A4

公开(公告)日：2017-03-22

申请号：EP14853879

申请日：2014-10-16

申请人： NAT UNIV CORP KUMAMOTO UNIV

发明人： NISHINAKAMURA RYUICHI ,  TAGUCHI ATSUHIRO

IPC分类号： C12N5/10 ,  C12N5/071 ,  C12N5/0735

CPC分类号： C12N5/0671 ,  C12N5/0672 ,  C12N2501/119 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/385 ,  C12N2501/415 ,  C12N2506/02 ,  C12N2506/45

摘要： The purpose of the present invention is to provide a process or method that can be utilized when deriving a three-dimensional structure of a kidney from pluripotent stem cells such as ES cells or iPS cells. The differentiation inducing method is characterized by culturing pluripotent cells with the following three steps, in order: (a) a step of culturing an embryoid body induced from the pluripotent stem cells in medium containing Bmp4 and a high-concentration (concentration A) Wnt agonist; (b) a step of culturing the embryoid body in medium which contains activin, Bmp4, retinoic acid and a middle-concentration (concentration B) Wnt agonist; and (c) a step of culturing the embryoid body in medium containing Fgf9 and a low-concentration (concentration C) Wnt agonist (herein, the Wnt agonist concentrations is concentration A > concentration B > concentration C).

摘要翻译： 本发明的目的是提供当从多能干细胞（例如ES细胞或iPS细胞）衍生肾的三维结构时可以使用的方法或方法。 分化诱导方法的特征在于以下三个步骤培养多能细胞：（a）在含有Bmp4和高浓度（浓度A）Wnt激动剂的培养基中培养由多能干细胞诱导的胚状体的步骤 ; （b）在含有激活素，Bmp4，视黄酸和中浓度（浓度B）Wnt激动剂的培养基中培养类胚体的步骤; 和（c）在含有Fgf9和低浓度（C浓度）Wnt激动剂的培养基中培养胚状体的步骤（Wnt激动剂浓度为浓度A>浓度B>浓度C）。

公开(公告)号：EP2376627B1

公开(公告)日：2017-03-15

申请号：EP09795527.2

申请日：2009-12-16

申请人： UCL Business PLC

发明人： SELDEN, Clare ,  ERRO, Eloy

IPC分类号： A61M1/34 ,  A61K35/12 ,  C12N5/071

CPC分类号： C12N5/0671 ,  A61K2035/126 ,  A61M1/3472 ,  A61M1/3489 ,  C12N2500/14 ,  C12N2501/91 ,  C12N2533/74

摘要： The present invention relates to a method of growing a plurality of cells (110) to performance competence in a matrix forming agent (120) containing one or more density modifiers (130), characterized in that the cells are grown in a culture media supplemented with from 2-20% human plasma. It also relates to a biological component (100) comprising: i. a matrix forming agent (120), ii. a plurality of cells (110), and iii. one or more density modifiers (130), wherein at performance competence, the cells are present in the matrix forming agent at a density of at least 3×107 cells/ml.

公开(公告)号：EP2471908B1

公开(公告)日：2016-10-05

申请号：EP10811643.5

申请日：2010-07-28

申请人： The University of Tokyo ,  LSI Medience Corporation

发明人： MATSUI, Hitoshi ,  SAKAI, Yasuyuki ,  FUJII, Teruo ,  TAKEUCHI, Shoji ,  TSUDA, Yukiko

IPC分类号： C12N5/071 ,  C12M1/34 ,  C12Q1/02

CPC分类号： C12M23/20 ,  C12M23/24 ,  C12M25/14 ,  C12M41/46 ,  C12N5/0671 ,  C12N2503/00 ,  C12N2533/30 ,  C12N2533/54 ,  G01N33/5044

公开(公告)号：EP3010556A1

公开(公告)日：2016-04-27

申请号：EP14731159.1

申请日：2014-06-16

申请人： Baer, Hans U.

发明人： Baer, Hans U.

IPC分类号： A61L27/26 ,  A61L27/38 ,  A61L27/56 ,  A61L27/58

CPC分类号： C12N5/0671 ,  A61L27/26 ,  A61L27/3691 ,  A61L27/3804 ,  A61L27/3886 ,  A61L27/3891 ,  A61L27/48 ,  A61L27/56 ,  A61L27/58 ,  A61L2400/18 ,  A61L2430/28 ,  C12N5/0677 ,  C12N2533/40 ,  C12N2533/54 ,  C08L67/04 ,  C08L89/06

摘要： The present invention relates to a porous matrix for tissue engineering comprising a first biodegradable and biocompatible polymer component forming a three-dimensional primary structure with primary pores, and further comprising a second biodegradable and biocompatible polymer component other than the first polymer component selected from the group consisting of collagens, laminin, fibronectin and mixtures thereof, wherein the second polymer component forms a three-dimensional secondary structure which is contained within the interior space of at least a part of the primary pores.

摘要翻译： 本发明涉及用于组织工程的多孔基质，其包含形成具有初级孔的三维一级结构的第一种可生物降解和生物相容的聚合物组分，并且还包含第二种可生物降解和生物相容的聚合物组分， 由胶原，层粘连蛋白，纤连蛋白及其混合物组成，其中第二聚合物组分形成三维二级结构，其包含在至少一部分初级孔的内部空间内。