摘要:
Diploid Pelargonium peltatum plants containing a factor resulting in male sterility and/or in their petals at least one of the anthocyanidins pelargonidin and paeonidin, and which may be propagated by seed, as well as the introduction of said characteristics into Pelargonium peltatum using plant breeding and tissue culture techniques.
摘要:
The present invention provides a plants which exhibit delayed or inhibited fruit ripening and/or vegetable senescence obtained by a method comprising:
(i) growing seedlings thereof in the presence of an auxin polar transport inhibitor and selecting those seedlings the hypocotyl of which grows at an acute angle of less than about 70° to the horizontal; and/or - (ii) contacting the seeds of plants which have a low or reduced polar auxin transport with a conjugation inhibitory amount of an inhibitor of cytokinin-N 7 ' 9 -conjugation and selecting from the thus contacted seeds those which germinate.
摘要:
A process for commercially propagating plants by tissue culture in such a way as both to conserve desired plant morphology and to transform the plant with respect to one or more desired genes. The method includes the steps of (a) creating an Agrobacterium vector containing the gene sequence desired to be transferred to the propagated plant, preferably together with a marker gene; (b) taking one or more petiole explants from a mother plant and inoculating them with the Agrobacterium vector; (c) conducting callus formation in the petiole sections in culture, in the dark; and (d) culturing the resulting callus in growth medium containing a benzylamino growth regulator such as benzylaminopurine or, most preferably, benzylaminopurine-riboside. Additional optional growth regulators including auxins and cytokinins (indole butyric acid, benzylamine, benzyladenine, benzylaminopurine, alpha naphthylacetic acid and others known in the art) may also be present. Preferably, the petiole tissue is taken from Pelargonium x domesticum and the Agrobacterium vector contains an antisense gene for ACC synthase or ACC oxidase to prevent ACC synthase or ACC oxidase expression and, in turn, the ethylene formation for which these enzymes are precursors.
摘要:
Methods of regenerating fertile Zea mays plants from protoplasts or protoplast-derived cells are described. The protoplasts or cells may be derived from embryogenic cell cultures or callus cultures. The protoplasts, cells and resulting plants may be transgenic, containing, for example, chimeric genes coding for a polypeptide having substantially the insect toxicity properties of the crystal protein produced by Bacillus thuringiensis .
摘要:
Compositions for and method of degrading organic chemicals in soil. The composition is a nutrient medium serving as a substrate for microorganisms in the soil, preferably containing a major proportion of a source of carbon skeleton and energy, a macronutrient component preferably including nitrogen and other macronutrients, and a micronutrient component, preferably also a complexing agent and a vitamin/co-factor component. This nutrient material is added to soil, e.g. soil contaminated by a pesticide, to cause proliferation of microorganisms which are effective, or which develop effectiveness to degrade the organic chemicals. Preferably the microorganisms are those naturally present in the soil but useful microorganisms may be added with the nutrient medium.
摘要:
A process for increasing the rate of plant growth. Plants are treated with one or more acids, which are condensation products of glycolic and/or L-lactic acid. These acids also increase the concentration of chlorophyl, increase the rate of new plant formation when plants are propagated by tissue culture, decrease the amount of added nutrients required for plant growth, and protect plants against the toxic effects of salts. Certain of the acids are useful for increasing the rate of root formation in the plant.
摘要:
A method for encouraging the production of secondary plant embryos from a culture of primary somatic embryos is characterized in that an adequate concentration of an embryogenesis-inhibiting protein is introduced into the culture medium of said primary somatic embryos for secondary embryogenesis.