公开(公告)号：EP2847336B1

公开(公告)日：2018-01-17

申请号：EP13726106.1

申请日：2013-05-07

申请人： Kochanek, Stefan

发明人： LUCAS, Tanja ,  KUEPPERS, Claudia ,  KOCHANEK, Stefan

IPC分类号： C12N15/861 ,  C12N5/10

CPC分类号： C12N15/85 ,  C12N15/86 ,  C12N15/861 ,  C12N2710/10343 ,  C12N2840/44 ,  C12N2840/85

摘要： The present invention is related to a nucleic acid construct comprising - an expression unit for the expression of E1B, wherein the expression unit comprises a promoter, a nucleotide sequence coding for E1B, and a 3'UTR, wherein the promoter is operatively linked to the nucleotide sequence coding for E1B, and wherein the 3'UTR comprises 30 or less than 30 Exonic Enhancer Elements (ESEs), preferably 20 or less than 20 Exonic Enhancer Elements (ESEs) and aminocytic cells containing said nucleic acid construct.

公开(公告)号：EP2792744B1

公开(公告)日：2017-07-26

申请号：EP12857876.2

申请日：2012-12-12

申请人： Public University Corporation Yokohama City University

发明人： ADACHI, Noritaka

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10

CPC分类号： C12N15/85 ,  C12N15/09 ,  C12N15/63 ,  C12N15/8509 ,  C12N15/907 ,  C12N2830/50 ,  C12N2840/203 ,  C12N2840/44

公开(公告)号：EP2185711B1

公开(公告)日：2017-05-10

申请号：EP08797373.1

申请日：2008-08-07

申请人： Wayne State University

发明人： CARLOCK, Leon ,  CYPHER, Maria

IPC分类号： C12N15/82 ,  A01H5/00

CPC分类号： C12N15/85 ,  A01K2217/052 ,  A01K2227/105 ,  A01K2267/0393 ,  A61K48/00 ,  A61K48/0066 ,  C07K14/4713 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/14043 ,  C12N2750/14143 ,  C12N2799/025 ,  C12N2799/026 ,  C12N2830/42 ,  C12N2840/00 ,  C12N2840/10 ,  C12N2840/203 ,  C12N2840/44 ,  C12N2840/85 ,  G01N33/5014 ,  G01N2333/90241 ,  G01N2510/00

公开(公告)号：EP2788489B1

公开(公告)日：2017-03-15

申请号：EP12856150.3

申请日：2012-10-16

申请人： Virovek, Inc.

发明人： CHEN, Haifeng

IPC分类号： C12N15/31 ,  C12N15/113 ,  C12N15/79 ,  C12N15/64 ,  C12N5/10 ,  A61P35/00 ,  A61K31/7088 ,  A61K35/76

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K48/00 ,  C07K14/21 ,  C07K14/34 ,  C12N9/22 ,  C12N9/2497 ,  C12N2710/14043 ,  C12N2710/14052 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2799/026 ,  C12N2830/42 ,  C12N2840/005 ,  C12N2840/102 ,  C12N2840/44 ,  C12N2840/85

公开(公告)号：EP2986635A1

公开(公告)日：2016-02-24

申请号：EP14721802.8

申请日：2014-04-18

申请人： Fondazione Telethon

发明人： AURICCHIO, Alberto ,  COLELLA, Pasqualina ,  TRAPANI, Ivana

IPC分类号： C07K14/705 ,  C12N15/86

CPC分类号： C12N15/86 ,  A61K48/005 ,  C07K14/705 ,  C12N2750/14143 ,  C12N2800/40 ,  C12N2840/20 ,  C12N2840/44 ,  C12N2840/445

摘要： The present invention relates to constructs, vectors, relative host cells and pharmaceutical compositions which allow an effective gene therapy, in particular of genes larger than 5 Kb.

摘要翻译： 本发明涉及允许有效基因治疗，特别是大于5Kb的基因的构建体，载体，相对宿主细胞和药物组合物。

公开(公告)号：EP2792744A4

公开(公告)日：2015-08-19

申请号：EP12857876

申请日：2012-12-12

申请人： PUBLIC UNIV CORP YOKOHAMA CITY

发明人： ADACHI NORITAKA

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10

CPC分类号： C12N15/85 ,  C12N15/09 ,  C12N15/63 ,  C12N15/8509 ,  C12N15/907 ,  C12N2830/50 ,  C12N2840/203 ,  C12N2840/44

公开(公告)号：EP1451295B1

公开(公告)日：2015-06-03

申请号：EP02780573.8

申请日：2002-11-04

申请人： Athersys, Inc.

发明人： HARRINGTON, John Joseph ,  JACKSON, Paul David ,  JIANG, Li

IPC分类号： C12N15/86

CPC分类号： C12N15/86 ,  A01K2217/075 ,  A01K2267/03 ,  C12N2740/10043 ,  C12N2800/60 ,  C12N2800/90 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44 ,  C12N2840/445

公开(公告)号：EP2788489A1

公开(公告)日：2014-10-15

申请号：EP12856150.3

申请日：2012-10-16

申请人： Virovek, Inc.

发明人： CHEN, Haigeng

IPC分类号： C12N15/31 ,  C12N15/113 ,  C12N15/79 ,  C12N15/64 ,  C12N5/10 ,  A61P35/00 ,  A61K31/7088 ,  A61K35/76

CPC分类号： C12N15/86 ,  A61K31/7088 ,  A61K48/00 ,  C07K14/21 ,  C07K14/34 ,  C12N9/22 ,  C12N9/2497 ,  C12N2710/14043 ,  C12N2710/14052 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2799/026 ,  C12N2830/42 ,  C12N2840/005 ,  C12N2840/102 ,  C12N2840/44 ,  C12N2840/85

摘要： The present disclosure describes nucleic acids, and viruses comprising such nucleic acids, for growing a toxic gene in an insect cell. These nucleic acids comprise a sequence encoding a toxic polypeptide, and an intron that interrupts the sequence, whereby the intron is spliced in mammalian cells but not in insect cells. Infection of mammalian cells but not insect cells with the nucleic acids or viruses can lead to expression of toxic levels of the toxic polypeptide in mammalian cells but not in insect cells. Viruses, such as an AAV or a baculovirus comprising a nucleic acid can be grown in insect cell lines in vitro and can be administered to a subject in need of therapy, such as a subject in need of cancer therapy.

公开(公告)号：EP1451316B1

公开(公告)日：2014-08-06

申请号：EP02766279.0

申请日：2002-09-12

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： BALTIMORE, David ,  HONG, Elizabeth, J. ,  LOIS-CABALLE, Carlos ,  PEASE, Shirley

IPC分类号： C12N15/00 ,  C12N15/85 ,  A01K67/027 ,  C12N15/867

CPC分类号： C12N15/86 ,  A01K2217/05 ,  A01K2227/30 ,  A01K2227/40 ,  A61K38/00 ,  A61K48/00 ,  C12N15/1132 ,  C12N15/1138 ,  C12N15/8509 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2330/30 ,  C12N2740/16043 ,  C12N2800/60 ,  C12N2830/003 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44 ,  Y02A50/463 ,  Y02A50/465

摘要： The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which are capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.

公开(公告)号：EP1098986B1

公开(公告)日：2011-12-28

申请号：EP99934910.3

申请日：1999-07-21

申请人： MILLIPORE CORPORATION

发明人： ANTONIOU, Michael, Dept. of Experimental Pathology ,  CROMBIE, Robert, Cobra Therapeutics Limited

IPC分类号： C12N15/85 ,  C12N5/10 ,  C12Q1/68 ,  A61K48/00 ,  C12N15/00 ,  A01K67/027 ,  C12N15/11 ,  C12N15/86

CPC分类号： C12N15/11 ,  A01K2217/05 ,  A61K48/00 ,  C12N15/63 ,  C12N15/85 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2830/008 ,  C12N2830/205 ,  C12N2830/42 ,  C12N2830/46 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12N2840/44

摘要： The present invention relates to a polynucleotide comprising a ubiquitous chromatin opening element (UCOE) which is not derived from an LCR. The present invention also relates to a vector comprising the polynucleotide sequence, a host cell comprising the vector, use of the polynucleotide, vector or host cell in therapy and in an assay, and a method of identifying UCOEs. The UCOE opens chromatin or maintains chromatin in an open state and facilitates reproducible expression of an operably-linked gene in cells of at least two different tissue types.