公开(公告)号：EP1362096A2

公开(公告)日：2003-11-19

申请号：EP02716797.2

申请日：2002-02-21

申请人： Kochanek, Stefan ,  Schraermeyer, Ulrich

发明人： KOCHANEK, Stefan ,  SCHRAERMEYER, Ulrich ,  THUMANN, Gabriele

IPC分类号： C12N5/10 ,  A61K35/44 ,  A61K48/00 ,  A61P27/02

CPC分类号： C12N15/86 ,  A61K35/30 ,  A61K38/57 ,  A61K48/00 ,  C12N5/0621 ,  C12N2503/02 ,  C12N2510/00 ,  C12N2710/10343 ,  C12N2830/008

摘要： The invention relates to a pigmentary epithelium cell of the eye, containing vector DNA of an adenoviral vector having a high DNA capacity. The invention also relates to the improved isolation and cultivation of said cells, methods for producing the same, and the use thereof for treating eye diseases or nerve diseases.

公开(公告)号：EP2847336A1

公开(公告)日：2015-03-18

申请号：EP13726106.1

申请日：2013-05-07

申请人： Kochanek, Stefan

发明人： LUCAS, Tanja ,  KUEPPERS, Claudia ,  KOCHANEK, Stefan

IPC分类号： C12N15/861 ,  C12N5/10

CPC分类号： C12N15/85 ,  C12N15/86 ,  C12N15/861 ,  C12N2710/10343 ,  C12N2840/44 ,  C12N2840/85

摘要： The present invention is related to a nucleic acid construct comprising - an expression unit for the expression of E1B, wherein the expression unit comprises a promoter, a nucleotide sequence coding for E1B, and a 3'UTR, wherein the promoter is operatively linked to the nucleotide sequence coding for E1B, and wherein the 3'UTR comprises 30 or less than 30 Exonic Enhancer Elements (ESEs), preferably 20 or less than 20 Exonic Enhancer Elements (ESEs) and aminocytic cells containing said nucleic acid construct.

摘要翻译： 本发明涉及核酸构建体，其包含 - 用于表达E1B的表达单元，其中所述表达单元包含启动子，编码E1B的核苷酸序列和3'UTR，其中所述启动子可操作地连接到 编码E1B的核苷酸序列，其中3'UTR包含30个或少于30个超声增强子元件（ESE），优选20个或少于20个超强度增强子元件（ESE）和含有该核酸构建体的氨基酸细胞。

公开(公告)号：EP2847336B1

公开(公告)日：2018-01-17

申请号：EP13726106.1

申请日：2013-05-07

申请人： Kochanek, Stefan

发明人： LUCAS, Tanja ,  KUEPPERS, Claudia ,  KOCHANEK, Stefan

IPC分类号： C12N15/861 ,  C12N5/10

CPC分类号： C12N15/85 ,  C12N15/86 ,  C12N15/861 ,  C12N2710/10343 ,  C12N2840/44 ,  C12N2840/85

摘要： The present invention is related to a nucleic acid construct comprising - an expression unit for the expression of E1B, wherein the expression unit comprises a promoter, a nucleotide sequence coding for E1B, and a 3'UTR, wherein the promoter is operatively linked to the nucleotide sequence coding for E1B, and wherein the 3'UTR comprises 30 or less than 30 Exonic Enhancer Elements (ESEs), preferably 20 or less than 20 Exonic Enhancer Elements (ESEs) and aminocytic cells containing said nucleic acid construct.

公开(公告)号：EP1230354A2

公开(公告)日：2002-08-14

申请号：EP00979539.4

申请日：2000-11-07

申请人： Kochanek, Stefan

发明人： KOCHANEK, Stefan ,  SCHIEDNER, Gudrun

IPC分类号： C12N15/00

CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2800/30 ,  C12N2810/40 ,  C12N2810/405 ,  C12N2810/60 ,  C12N2810/6018 ,  C12N2810/859

摘要： The invention relates to a permanent amniocyte cell line containing at least one nucleic acid, which effects the expression of gene products of the E1A region and E1B region of andenovirus. The invention also relates to the production of a permanent amniocyte cell line and to its use for producing gene transfer vectors. Additional aspects include the use of amniocytes or of adenoviral gene products of the E1A region and E1B region for producing permanent amniocyte cell lines.