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    SMALL APERTURE LARGE ELECTRODE NANOPORE CELL AND METHOD OF MANUFACTURE
    16.
    发明公开
    SMALL APERTURE LARGE ELECTRODE NANOPORE CELL AND METHOD OF MANUFACTURE 审中-公开

    公开(公告)号：EP3344993A1

    公开(公告)日：2018-07-11

    申请号：EP16766235.2

    申请日：2016-08-26

    申请人： Genia Technologies, Inc. ,  Roche Diagnostics GmbH

    发明人： FOSTER, John ,  MAGER, Morgan

    IPC分类号： G01N33/487

    CPC分类号： G01N33/48721 ,  G01N27/44791 ,  H01L29/401

    摘要： A nanopore cell includes a conductive layer and a working electrode disposed above the conductive layer and at the bottom of a well into which an electrolyte may be contained, such that at least a portion of a top base surface area of the working electrode is exposed to the electrolyte. The nanopore cell further includes a first insulating wall disposed above the working electrode and surrounding a lower section of a well, and a second insulating wall disposed above the first insulating wall and surrounding an upper section of the well, forming an overhang above the lower section of the well. The upper section of the well includes an opening that a membrane may span across, and wherein a base surface area of the opening is smaller than the at least a portion of the top base surface area of the working electrode that is exposed to the electrolyte.

    CHEMICAL METHODS FOR PRODUCING TAGGED NUCLEOTIDES
    17.
    发明公开
    CHEMICAL METHODS FOR PRODUCING TAGGED NUCLEOTIDES 审中-公开
    CHEMISCHE VERFAHREN ZUR HERSTELLUNG VON MARKIERTEN NUKLEOTIDEN

    公开(公告)号：EP3122759A1

    公开(公告)日：2017-02-01

    申请号：EP15768383.0

    申请日：2015-03-23

    申请人： The Trustees of Columbia University in the City of New York ,  Genia Technologies, Inc.

    发明人： FULLER, Carl, W. ,  KUMAR, Shiv ,  JU, Jingyue ,  DAVIS, Randall ,  CHEN, Roger

    IPC分类号： C07H19/04

    CPC分类号： C12Q1/6874 ,  C07H17/02 ,  C07H19/10 ,  C07H19/20 ,  C12Q1/6806

    摘要： This disclosure provides systems and methods for attaching nano pore-detectable tags to nucleotides. The disclosure also provides methods for sequencing nucleic acids using the disdosed tagged nudeotides. Provided herein are nudeotides with attached tags and methods for attaching tags to nudeotides. The tags can be attached by chemical reactions, such as "dick chemistry". In an aspect, the present disdosure provides a tagged nudeotide, comprising: (a) a poly-phosphate moiety having a terminal phosphate; and (b) a tag covalently coupled to the terminal phosphate of the nudeotide by a triazole, a 1,2-diazine, a disulfide, a secondary amine, a hydrazone, a thio-acetamide, or a maleimide-thioadduct.

    摘要翻译： 本公开提供了将纳米孔可检测标签附着于核苷酸的系统和方法。 本公开还提供了使用公开的标记核苷酸对核酸进行测序的方法。

    NANOPORE BASED MOLECULAR DETECTION AND SEQUENCING
    19.
    发明公开
    NANOPORE BASED MOLECULAR DETECTION AND SEQUENCING 审中-公开
    鉴定及测序分子对纳米多孔质基体

    公开(公告)号：EP2807476A1

    公开(公告)日：2014-12-03

    申请号：EP13738391.5

    申请日：2013-01-18

    申请人： Genia Technologies, Inc.

    发明人： DAVIS, Randall ,  CHEN, Roger

    IPC分类号： G01N27/26 ,  C12Q1/68 ,  G01N27/414

    CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2565/607

    摘要： This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.

    SYSTEMS AND METHODS FOR MANIPULATING A MOLECULE IN A NANOPORE
    20.
    发明公开
    SYSTEMS AND METHODS FOR MANIPULATING A MOLECULE IN A NANOPORE 审中-公开
    系统在VERFAHREN ZUR操作系列EINESMOLEKÜLSIN EINER NANOPORE

    公开(公告)号：EP2534284A1

    公开(公告)日：2012-12-19

    申请号：EP11740145.5

    申请日：2011-02-04

    申请人： Genia Technologies Inc.

    发明人： CHEN, Roger ,  DAVIS, Randy

    IPC分类号： C40B30/00 ,  C40B40/04

    CPC分类号： G01N33/48721 ,  C12Q1/6869 ,  C12Q2523/305 ,  C12Q2563/116 ,  C12Q2565/631

    摘要： Techniques for manipulating a molecule in a nanopore embedded in a lipid bilayer are described. In one example, an acquiring electrical stimulus level is applied across a lipid bilayer wherein a region of the lipid bilayer containing the nanopore is characterized by a resistance and wherein the acquiring electrical stimulus level tends to draw the molecule from a surrounding fluid into the nanopore, a change in the resistance of the lipid bilayer resulting from the acquisition of at least a portion of a molecule into the nanopore is detected, the acquiring electrical stimulus level is changed to a holding electrical stimulus level wherein the portion of the molecule remains in the nanopore upon the changing of the acquiring electrical stimulus level to the holding electrical stimulus level.

    摘要翻译： 描述了在嵌入脂质双层中的纳米孔中操纵分子的技术。 在一个实例中，获得电刺激水平跨越脂质双层施加，其中包含纳米孔的脂质双层的区域的特征在于阻力，并且其中获取电刺激水平倾向于将分子从周围流体吸引到纳米孔中， 检测到从将至少一部分分子获得到纳米孔中获得的脂质双层的电阻的变化，将获得的电刺激水平改变为保持电刺激水平，其中分子的部分保留在纳米孔中 将获取的电刺激水平改变为保持电刺激水平。