公开(公告)号：EP3155000A1

公开(公告)日：2017-04-19

申请号：EP15730106.0

申请日：2015-06-12

申请人： SESVanderHave N.V.

发明人： WEYENS, Guy ,  LEFÈBVRE, Marc ,  THAMMANNAGOWDA, Shivegowda ,  LOMMEL, Murielle

IPC分类号： C07K14/415 ,  C12N15/82

CPC分类号： C12N15/821 ,  A01H1/02 ,  A01H1/04 ,  C12N9/88 ,  C12N15/52 ,  C12N15/8209 ,  C12N15/8241 ,  C12N15/8279 ,  C12N15/8281 ,  C12N15/8282 ,  C12N15/8285 ,  C12N15/8286

摘要： A method for the transformation of sugar beet protoplasts includes obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant. The protoplasts are transformed with a nucleic acid construct including a nucleotide sequence of interest and a selection marker sequence. One or more ALS inhibitors at a concentration that is lethal to the in vitro culture of the protoplasts are applied to an in vitro culture of the protoplasts. Sugar beet plants are regenerated from the surviving protoplasts having integrated the nucleic acid construct including the sequence of interest and the selection marker sequence. The selection marker sequence is the mutated BvALS113 sequence carrying in its sequence a mutation at amino acid 113 position from Alanine to Tyrosine.

摘要翻译： 本发明涉及一种用于转化甜菜原生质体的方法，包括以下步骤： - 从分离自甜菜植物的气孔保护细胞获得原生质体， - 用包含感兴趣的核苷酸序列的核酸构建体转化原生质体，以及 选择标记序列， - 应用于所述原生质体的体外培养，一种或多种浓度对原生质体的体外培养致死的ALS抑制剂，以及从具有整合核酸的存活的原生质体再生甜菜植物 构建体，其包含感兴趣的序列和选择标记序列，其中选择标记序列是突变的BvALS113序列，其在氨基酸113位置从丙氨酸到酪氨酸的突变。

公开(公告)号：EP2931902A1

公开(公告)日：2015-10-21

申请号：EP13819001.2

申请日：2013-12-13

申请人： SESVanderHave N.V.

发明人： WEYENS, Guy ,  LEFÈBVRE, Marc ,  HAIN, Rüdiger ,  JOHANN, Gerhard

IPC分类号： C12N15/82 ,  A01H3/04 ,  A01H4/00 ,  A01H5/06

CPC分类号： A01H4/008 ,  A01H3/04 ,  A01H4/00 ,  C12N15/8274 ,  C12N15/8278

摘要： A method for producing herbicide-resistant sugar beet plant includes obtaining protoplasts from stomatal guard cells isolated from a sugar beet plant. A composition having an ALS herbicide is applied to the cells at a concentration which is lethal to the cells. Sugar beet plants are regenerated from the surviving cells.

摘要翻译： 用于制备除草剂抗性甜菜植物的方法包括从从甜菜植物分离的气孔保护细胞获得原生质体。 将具有ALS除草剂的组合物以对细胞致死的浓度施用于细胞。 甜菜植物从存活细胞中再生。

公开(公告)号：EP2723873A1

公开(公告)日：2014-04-30

申请号：EP12728499.0

申请日：2012-06-15

申请人： SESVanderHave N.V.

发明人： KLEIN, Elodie ,  GRAFF, Véronique ,  GILMER, David ,  BRAULT, Véronique ,  WEYENS, Guy ,  LEFEBVRE, Marc

IPC分类号： C12N15/82

摘要： The present invention is related to a recombinant BMYV P0 viral nucleotide sequence when transcribed in a cell is capable of forming a double stranded self-complementary RNA sequence.

摘要翻译： 当在细胞中转录能够形成双链自身互补RNA序列时，本发明涉及重组BMYV P0病毒核苷酸序列。

公开(公告)号：EP2013231A2

公开(公告)日：2009-01-14

申请号：EP07728713.4

申请日：2007-05-02

申请人： Sesvanderhave N.V.

发明人： LAUBER, Emmanuelle ,  GUILLEY, Hubert ,  RICHARDS, Ken ,  JONARD, Gerard ,  KLEIN, Elodie ,  GILMER, David

IPC分类号： C07K14/08

CPC分类号： C12N15/8283

摘要： The invention relates to genetically modified TGB- 3 viral sequences suitable to induce gene silencing. In particular, hairpin constructs based on such sequences proved highly efficient to induce a PTGS mechanism and degradation of the whole of RNA2 thereby. When plants are transformed accordingly, the spread of the virus in the plant is significantly reduced or blocked. The invention relates of the hairpin constructs, their use and to plants, plant cells and plant tissues harboring these constructs.