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11.发明公开Detection of nucleic and sequence differences using the ligase detection reaction with addressable arrays 失效通过寻址阵列和Ligasedetektionsreaktion来检测在核酸序列的差异
公开(公告)号：EP2332958A1
公开(公告)日：2011-06-15
申请号：EP10075458.9
申请日：1997-02-05
申请人： Cornell Research Foundation, Inc. , REGENTS OF THE UNIVERSITY OF MINNESOTA , Louisiana State University
发明人： Barany, Francis , Barany, George , Hammer, Robert P. , Kempe, Maria , Blok, Herman , Zirvi, Monib
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00536 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00729 , B82Y30/00 , C07B2200/11 , C12Q1/6837 , C12Q1/689 , C40B20/02 , C40B50/14 , Y02A50/58 , C12Q2565/513 , C12Q2561/125
摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.
摘要翻译： 本发明描述了用于识别一个或多个由一个或多个单碱基变化，插入，缺失，易位或在靶核苷酸序列的多种不同序列的多个方法。 该方法包括：一个相连接，一个捕获阶段和检测阶段。 结扎相位利用一个寡核苷酸探针，其具有可寻址的阵列特异性部分的靶序列特异性部分和探针，和第二寡核苷酸之间的连接检测反应，具有目标序列特异性部分和可检测标记。 结扎阶段之后，捕获相位由连接的寡核苷酸探针与固定化在捕获寡核苷酸的阵列的至少一些是可寻址的阵列特异性部分互补杂交的固体支持体上进行。 以下捕获阶段完成后，检测相位进行检测杂交至在固体支持连接的寡核苷酸探针的标签。 结扎相可通过在扩增过程之前。 因此，本发明涉及一种试剂盒，用于实施该方法中，形成在固体载体上阵列的方法，以及支持他们自己。
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12.发明授权Detection of nucleic and acid sequence differences using the ligase detection reaction with addressable arrays 失效通过寻址阵列和Ligasedetektionsreaktion来检测在核酸序列的差异
公开(公告)号：EP2332957B1
公开(公告)日：2015-04-08
申请号：EP10075459.7
申请日：1997-02-05
申请人： Cornell Research Foundation, Inc. , REGENTS OF THE UNIVERSITY OF MINNESOTA , Louisiana State University
发明人： Barany, Francis , Barany, George , Hammer, Robert P. , Kempe, Maria , Blok, Herman , Zirvi, Monib
CPC分类号： B01J19/0046 , B01J2219/00432 , B01J2219/00527 , B01J2219/00529 , B01J2219/00536 , B01J2219/00585 , B01J2219/0059 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00711 , B01J2219/00722 , B01J2219/00729 , B82Y30/00 , C07B2200/11 , C12Q1/6837 , C12Q1/689 , C40B20/02 , C40B50/14 , Y02A50/58 , C12Q2565/513 , C12Q2561/125
摘要： The present invention describes a method for identifying one or more of a plurality of sequences differing by one or more single base changes, insertions, deletions, or translocations in a plurality of target nucleotide sequences. The method includes a ligation phase, a capture phase, and a detection phase. The ligation phase utilizes a ligation detection reaction between one oligonucleotide probe, which has a target sequence-specific portion and an addressable array-specific portion, and a second oligonucleotide probe, having a target sequence-specific portion and a detectable label. After the ligation phase, the capture phase is carried out by hybridizing the ligated oligonucleotide probes to a solid support with an array of immobilized capture oligonucleotides at least some of which are complementary to the addressable array-specific portion. Following completion of the capture phase, a detection phase is carried out to detect the labels of ligated oligonucleotide probes hybridized to the solid support. The ligation phase can be preceded by an amplification process. The present invention also relates to a kit for practicing this method, a method of forming arrays on solid supports, and the supports themselves.
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13.发明授权Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 失效确定使用Ligasebestimmung和聚合酶链反应的组合中的核酸序列的差异
公开(公告)号：EP1736554B1
公开(公告)日：2013-10-09
申请号：EP06011667.0
申请日：1997-05-27
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
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14.发明公开Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 失效确定使用Ligasebestimmung和聚合酶链反应的组合中的核酸序列的差异
公开(公告)号：EP1736554A2
公开(公告)日：2006-12-27
申请号：EP06011667.0
申请日：1997-05-27
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
摘要翻译： 本发明涉及到检测的使用耦合连接酶检测反应和聚合酶链式反应的核酸序列的差异。 本发明的一个方面涉及使用耦合到聚合酶链式反应中的连接酶检测反应的。 本发明的另一个方面涉及使用耦合到耦合到连接酶检测反应的二次聚合酶链式反应的初级聚合酶链式反应。 本发明的第三方面涉及耦合到次级聚合酶链反应的初级聚合酶链式反应。 在连接酶检测反应和聚合酶链式反应的搜索耦合允许的核酸序列差异多重检测。
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15.发明授权Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 失效通过同时Ligaseerkennungs-和聚合酶链反应的手段的核酸序列的差异检测
公开(公告)号：EP2369007B1
公开(公告)日：2015-07-29
申请号：EP10075461.3
申请日：1997-05-27
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
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16.发明公开Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 失效通过同时Ligaseerkennungs-和聚合酶链反应的手段的核酸序列的差异检测
公开(公告)号：EP2369007A1
公开(公告)日：2011-09-28
申请号：EP10075461.3
申请日：1997-05-27
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
摘要翻译： 本发明涉及到检测的使用耦合连接酶检测反应和聚合酶链式反应的核酸序列的差异。 本发明的一个方面涉及使用耦合到聚合酶链式反应中的连接酶检测反应的。 本发明的另一个方面涉及使用耦合到耦合到连接酶检测反应的二次聚合酶链式反应的初级聚合酶链式反应。 本发明的第三方面涉及耦合到次级聚合酶链反应的初级聚合酶链式反应。 在连接酶检测反应和聚合酶链式反应的搜索耦合允许的核酸序列差异多重检测。
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17.发明公开Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions 失效通过Ligasebestimmung的组合和聚合酶链反应确定所述Nukleisäuresequenz差异
公开(公告)号：EP1736554A3
公开(公告)日：2007-02-21
申请号：EP06011667.0
申请日：1997-05-27
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C12Q1/6813 , C12Q1/6827 , C12Q1/683 , C12Q1/6851 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6862 , C12Q1/6869 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16 , C12Q2561/125 , C12Q2531/113 , C12Q2525/161 , C12Q2545/114 , C12Q2535/131 , C12Q2521/501 , C12Q2537/143 , C12Q2533/107 , C12Q2525/131 , C12Q2525/125 , C12Q2521/319 , C12Q2521/531 , C12Q2525/155
摘要： The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences.
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18.发明授权A thermostable ligase mediated DNA amplification system for the detection of genetic diseases 失效含有DNA扩增系统，用于遗传性疾病的确定热稳定连接酶
公开(公告)号：EP1507000B1
公开(公告)日：2010-03-03
申请号：EP04077667.6
申请日：1991-04-29
CPC分类号： C12Q1/6858 , C12N9/93 , C12N15/10 , C12Q1/6862 , C12Q2600/156 , C12Q2531/137 , C12Q2535/131 , C12Q2527/101 , C12Q2521/501
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19.发明公开A thermostable ligase mediated DNA amplification system for the detection of genetic diseases 失效含有DNA扩增系统用于遗传Krankeiten测定热稳定连接酶
公开(公告)号：EP1507000A3
公开(公告)日：2006-03-29
申请号：EP04077667.6
申请日：1991-04-29
CPC分类号： C12Q1/6858 , C12N9/93 , C12N15/10 , C12Q1/6862 , C12Q2600/156 , C12Q2531/137 , C12Q2535/131 , C12Q2527/101 , C12Q2521/501
摘要： The present invention relates to the cloning of the gene of a thermophilic DNA ligase, from Thermus aquaticus strain HB8, and the use of this ligase for the detection of specific sequences of nucleotides in a variety of nucleic acid samples, and more particularly in those samples containing a DNA sequence characterized by a difference in the nucleic acid sequence from a standard sequence including single nucleic acid base pair changes, deletions, insertions or translocations.
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20.发明公开A thermostable ligase mediated DNA amplification system for the detection of genetic diseases 失效Hitzestabile Ligase enthaltende DNS-Amplifizierungssystem zur Bestimmung von genetischen Krankeiten
公开(公告)号：EP1507000A2
公开(公告)日：2005-02-16
申请号：EP04077667.6
申请日：1991-04-29
CPC分类号： C12Q1/6858 , C12N9/93 , C12N15/10 , C12Q1/6862 , C12Q2600/156 , C12Q2531/137 , C12Q2535/131 , C12Q2527/101 , C12Q2521/501
摘要： The present invention relates to the cloning of the gene of a thermophilic DNA ligase, from Thermus aquaticus strain HB8, and the use of this ligase for the detection of specific sequences of nucleotides in a variety of nucleic acid samples, and more particularly in those samples containing a DNA sequence characterized by a difference in the nucleic acid sequence from a standard sequence including single nucleic acid base pair changes, deletions, insertions or translocations.
摘要翻译： 本发明涉及从水生栖热菌菌株HB8的嗜热DNA连接酶的基因的克隆，以及这种连接酶用于检测各种核酸样品中特定的核苷酸序列的用途，更具体地，涉及那些样品 其含有以包含单核酸碱基对变化，缺失，插入或易位的标准序列的核酸序列差异为特征的DNA序列。
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