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    IN VITRO RECOMBINATION METHOD
    4.
    发明授权
    IN VITRO RECOMBINATION METHOD 有权
    体外重组方法

    公开(公告)号：EP1915446B1

    公开(公告)日：2017-06-14

    申请号：EP06813388.3

    申请日：2006-08-11

    申请人： Synthetic Genomics, Inc.

    发明人： YOUNG, Lei ,  SMITH, Hamilton, O. ,  GIBSON, Daniel G.

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C12N15/64 ,  C12N15/66 ,  C12N15/10

    CPC分类号： C12N15/902 ,  C12N9/1252 ,  C12N9/22 ,  C12N9/93 ,  C12N15/10 ,  C12N15/64 ,  C12N15/66 ,  C12P19/34 ,  C12Q1/62 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q2521/501 ,  C12Q2522/101 ,  C12Y207/07007 ,  C12Y301/11003 ,  C12Y605/01001

    摘要： The present invention relates, e.g., to in vitro method, using isolated protein reagents, for joining two double stranded (ds) DNA molecules of interest, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule share a region of sequence identity, comprising contacting the two DNA molecules in a reaction mixture with (a) a non-processive 5′ exonculease; (b) a single stranded DNA binding protein (SSB) which accelerates nucleic acid annealing; (c) a non strand-displacing DNA polymerase; and (d) a ligase, under conditions effective to join the two DNA molecules to form an intact double stranded DNA molecule, in which a single copy of the region of sequence identity is retained. The method allows the joining of a number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes.

    摘要翻译： 本发明涉及例如使用分离的蛋白质试剂的体外方法用于连接两个感兴趣的双链（ds）DNA分子，其中第一个DNA分子的远侧区域和第二个DNA分子的近侧区域共享 包括使两种DNA分子在反应混合物中与（a）非进行型5'外切核酸酶; （b）加速核酸退火的单链DNA结合蛋白（SSB）; （c）非链置换DNA聚合酶; 和（d）连接酶，在有效连接两个DNA分子以形成完整的双链DNA分子的条件下，其中保留了序列同一性区域的单一拷贝。 该方法允许以预定的顺序和方向连接许多DNA片段，而不使用限制性内切酶。

    METHOD FOR MULTIPLEX NUCLEIC ACID ANALYSIS
    7.
    发明公开
    METHOD FOR MULTIPLEX NUCLEIC ACID ANALYSIS 有权
    VERFAHREN ZUR MULTIPLEX-NUKLEINSÄUREANALYSE

    公开(公告)号：EP2893034A1

    公开(公告)日：2015-07-15

    申请号：EP12883998.2

    申请日：2012-09-10

    申请人： Genesky Diagnostics (Suzhou) Inc. ,  Shanghai Genesky Biotechnologies Inc.

    发明人： JIANG, Zhengwen ,  YU, Feng ,  LI, Caihua

    IPC分类号： C12Q1/68 ,  G01N21/64

    CPC分类号： C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6862 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2525/204 ,  C12Q2537/143 ,  C12Q2561/125 ,  C12Q2535/125 ,  C12Q2525/155 ,  C12Q2537/16 ,  C12Q2563/107 ,  C12Q2565/125

    摘要： Here provided is a method for multiplex nucleic acid analysis. The method includes steps of hybridizing sets of probes to target nucleic acids in a sample, ligating the hybridized probes, amplifying the ligated probes, and assaying the amplification products to determining the presence, absence, or quantity of the target nucleic acids in the sample. The multiplexity is made available in part by adding detectable moieties and inserting stuffer sequences in primers so that amplification products may be identified on the basis of the detectable moieties and fragment sizes. Also provided is a sensitive method of detecting small copy number changes by measuring the copy number of a plurality of target sites in the nucleic acid in a test sample in comparison to a control sample and then determining the copy number of the nucleic acid based on the measured copy number of the plurality of target sites. Further provided is a kit for multiplex nucleic acid analysis and for small copy number change determination.

    摘要翻译： 这里提供了多重核酸分析的方法。 该方法包括将探针组与样品中的靶核酸杂交，连接杂交探针，扩增连接的探针和测定扩增产物以确定样品中目标核酸的存在，不存在或数量的步骤。 通过添加可检测的部分并在引物中插入填充序列，可以部分地获得多重性，使得可以基于可检测部分和片段大小来鉴定扩增产物。 还提供了通过测量测试样品中的核酸中的多个靶位点的拷贝数与对照样品相比较来检测小拷贝数​​变化的敏感方法，然后基于对照样品确定核酸的拷贝数 测量多个目标站点的拷贝数。 还提供了用于多重核酸分析和小拷贝数变化测定的试剂盒。

    METHODS AND COMPOSITIONS FOR DETECTING TARGET NUCLEIC ACIDS
    8.
    发明公开
    METHODS AND COMPOSITIONS FOR DETECTING TARGET NUCLEIC ACIDS 有权
    VERFAHREN UND ZUSAMMENSETZUNGEN ZUM NACHWEIS VONZIELNUKLEINSÄUREN

    公开(公告)号：EP2710145A1

    公开(公告)日：2014-03-26

    申请号：EP12723799.8

    申请日：2012-05-17

    申请人： Dxterity Diagnostics Incorporated

    发明人： TERBRUEGGEN, Robert ,  LIU, Yenbou ,  CHILDERS, John, Ray ,  KIM, Chang, Hee ,  ABEDI, Majid R.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6813 ,  C12Q1/6809 ,  C12Q1/6834 ,  C12Q1/6855 ,  C12Q1/6862 ,  C12Q2523/109 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/197 ,  C12Q2525/204 ,  C12Q2533/107 ,  C12Q2537/143 ,  C12Q2563/131 ,  C12Q2563/179 ,  C12Q2565/125 ,  C12Q2525/15

    摘要： The present invention provides compositions, apparatuses and methods for detecting one or more nucleic acid targets present in a sample. Methods of the invention include utilizing two or more ligation probes that reversibly bind a target nucleic acid in close proximity to each other and possess complementary reactive ligation moieties. When such probes have bound to the target in the proper orientation, they are able to undergo a spontaneous chemical ligation reaction that yields a ligation product that is directly detected or that is amplified to produce amplicons that are then detected. The present invention also provides methods to stabilize sample RNA so that degradation does not significantly affect the results of the analysis.

    摘要翻译： 本发明提供了用于检测样品中存在的一种或多种核酸靶的组合物，装置和方法。 本发明的方法包括使用两个或更多个连接探针，其可逆地结合彼此紧邻的靶核酸并具有互补的反应性连接部分。 当这样的探针以正确的方向结合靶时，它们能够进行自发化学连接反应，产生直接检测到的连接产物或被扩增以产生随后检测到的扩增子的连接产物。 本发明还提供了稳定样品RNA以使降解不显着影响分析结果的方法。