-
公开(公告)号：EP0459532B1
公开(公告)日：1995-07-19
申请号：EP91113105.0
申请日：1987-03-13
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C07K14/70539 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6881 , C12Q1/6883 , C12Q2600/156
-
公开(公告)号：EP0509612A3
公开(公告)日：1994-01-19
申请号：EP92201243.0
申请日：1986-03-27
发明人： Mullis, Kary Banks , Arnheim, Norman , Saiki, Randall Keichi , Erlich, Henry Anthony , Horn, Glenn Thomas , Scharf, Stephen Joel
CPC分类号： C12N15/10 , B01J2219/00495 , B01J2219/0059 , B01J2219/00722 , B01L7/52 , C07K14/805 , C12Q1/6853 , C12Q1/6858 , C12Q1/686 , C12Q1/6883 , C12Q1/6886 , C12Q1/689 , C40B40/06 , C40B60/14 , C12Q2525/131 , C12Q2521/513
摘要： The present invention provides a process for detecting the presence or absence of at least one specific nucleic acid sequence in a sample or distinguishing between two different nucleic acid sequences in said sample, wherein each nucleic acid sequence to be detected consists of two separate strands, which process comprises amplifying the specific sequence or sequences (if present) by
(a) treating the sample with one oligonucleotide primer for each of the two strands of each different specific nucleic acid sequence being detected under hybridizing conditions such that for each strand of each different sequence being detected an extension product of each primer is synthesized which is complementary to each nucleic acid strand, wherein said primers are selected so as to be substantially complementary to each strand of each specific sequence such that the extension product synthesized from one primer, when it is separated from its complement, serves as a template for synthesis of an extension product of the other primer; (b) treating the product of step (a) under denaturing conditions to separate the primer extension products from their templates; (c) treating the product of step (b) with oligonucleotide primers such that a primer extension product is synthesized using each of the single strands produced in step (b) as a template; and detecting the thus amplified sequence or sequences (if present).摘要翻译： 本发明提供了用于检测样品中是否存在至少一种特定核酸序列或区分所述样品中的两种不同核酸序列的方法，其中待检测的每种核酸序列由两种分开的链组成， 方法包括通过以下方法扩增特定序列（如果存在的话）：（a）在杂交条件下用每种不同特异性核酸序列的两条链中的每条寡核苷酸引物对一种寡核苷酸引物处理样品，使得对于每种不同序列的每条链 检测到与每条核酸链互补的每个引物的延伸产物，其中选择所述引物以便与每个特定序列的每条链基本互补，从而当从一条引物合成的延伸产物是 从它的补充中分离出来，作为合成扩展的模板 另一种底漆的离子产物; （b）在变性条件下处理步骤（a）的产物以将引物延伸产物与它们的模板分离; （c）用寡核苷酸引物处理步骤（b）的产物，从而使用步骤（b）中产生的每条单链作为模板合成引物延伸产物; 并检测如此扩增的序列（如果存在）。
-
13.发明公开Probe groups for the detection of nucleotide variations and genetic polymorphisms 失效Probengruppen zur Bestimmung von Nukleotidvariationen und genetischen Polymorphismen。
公开(公告)号：EP0459533A2
公开(公告)日：1991-12-04
申请号：EP91113236.3
申请日：1987-03-13
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C07K14/70539 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6881 , C12Q1/6883 , C12Q2600/156
摘要： The invention provides a group of oligonucleotide probes in which the individual probes are capable of hybridizing to specific gene sequence variations so as to permit detection of said variations when said probes are labelled and so-hybridized, whereby allelic variations and gene polymorphism in samples may be detected using said group of probes. Such probe groups are useful for detecting nucleotide variations, mutations and polymorphisms by hybridization to amplified nucleic acid sequence containing such variations, mutations or polymorphisms.
摘要翻译： 本发明提供了一组寡核苷酸探针，其中各个探针能够与特定的基因序列变异杂交，以便当所述探针被标记并如此杂交时允许检测所述变体，由此样品中的等位基因变异和基因多态性可以是 使用所述探针组进行检测。 这样的探针组可用于通过与含有这种变异，突变或多态性的扩增的核酸序列杂交来检测核苷酸变异，突变和多态性。
-
14.发明公开
公开(公告)号：EP0459532A2
公开(公告)日：1991-12-04
申请号：EP91113105.0
申请日：1987-03-13
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C07K14/70539 , C12Q1/6827 , C12Q1/6858 , C12Q1/686 , C12Q1/6881 , C12Q1/6883 , C12Q2600/156
摘要： The invention provides an oligonucleotide primer exhibiting complementarity to an end of a nucleic acid sequence corresponding to a second exon sequence in an HLA Class II gene, wherein said primer can be hybridized to said sequence and then extended thereon to produce a single stranded extension product which includes a sequence complementary to said second exon sequence. Such primers can be used in a nucleic acid amplification process to enable detection of gene variations or polymorphism. For detection purposes, the invention also includes an oligonucleotide probe that is capable of hybridizing to a sequence in the second exon of an HLA Class II gene so as to permit detection of said sequence when said probe is labelled as so-hybridized.
摘要翻译： 本发明提供了与HLA II类基因中对应于第二外显子序列的核酸序列的末端互补的寡核苷酸引物，其中所述引物可以与所述序列杂交，然后在其上延伸以产生单链延伸产物，其中 包括与所述第二外显子序列互补的序列。 这样的引物可用于核酸扩增过程，以检测基因变异或多态性。 为了检测目的，本发明还包括能够与HLA II类基因的第二外显子中的序列杂交的寡核苷酸探针，以便当所述探针被标记为如此杂交时能够检测所述序列。
-
15.发明公开
公开(公告)号：EP0200362A2
公开(公告)日：1986-12-10
申请号：EP86302298.4
申请日：1986-03-27
发明人： Mullis, Kary Banks , Arnheim, Norman , Saiki, Randall Keichi , Erlich, Henry Anthony , Horn, Glenn Thomas , Scharf, Stephen Joel
摘要： The present invention is directed to a process for amplifying and detecting any target nucleic acid sequence contained in a nucleic acid or mixture thereof. The process comprises treating separate complementary strands of the nucleic acid with a molar excess of two oligonucleotide primers, extending the primers to form complementary primer extension products which act as templates for synthesizing the desired nucleic acid sequence, and detecting the sequence so amplified. The steps of the reaction may be carried out stepwise or simultaneously and can be repeated as often as desired. In addition, a specific nucleic acid sequence may be cloned into a vector by using primers to amplify the sequence, which contain restriction sites on their non-complementary ends, and a nucleic acid fragment may be prepared from an existing shorter fragment using the amplification process.
摘要翻译： 本发明涉及用于扩增和检测核酸中包含的任何靶核酸序列或其混合物的方法。 该方法包括用摩尔过量的两种寡核苷酸引物处理核酸的单独互补链，延伸引物以形成互补引物延伸产物，其作为用于合成所需核酸序列的模板，并检测如此扩增的序列。 反应的步骤可以逐步或同时进行，并且可以根据需要经常重复。 另外，可以通过使用引物扩增该序列来将特定的核酸序列克隆到载体中，所述序列在其非互补末端包含限制性位点，并且可以使用扩增过程从现有的较短片段制备核酸片段 。
-
-
-
-
搜索结果
15 条记录 (耗时 0.027 秒)
