公开(公告)号：EP2455458B1

公开(公告)日：2015-01-07

申请号：EP11005045.7

申请日：2004-01-30

申请人： PROMEGA CORPORATION

发明人： Wood, Keith, V. ,  Los, Georgyi, V ,  Bulleit, Robert, F. ,  Klaubert, Dieter ,  McDougall, Mark ,  Zimprich, Chad

IPC分类号： C12N9/14 ,  C12N9/86 ,  C12Q1/34 ,  G01N33/50 ,  G01N33/58

CPC分类号： C12Q1/34 ,  C07C217/08 ,  C07C271/16 ,  C07D209/48 ,  C07D233/64 ,  C07D311/78 ,  C07D311/82 ,  C07D405/06 ,  C07D493/10 ,  C07D495/04 ,  C07F5/022 ,  C12N9/14 ,  C12N9/86 ,  C12Y308/01005 ,  G01N33/5005 ,  G01N33/58 ,  Y10S436/80

公开(公告)号：EP2395358A3

公开(公告)日：2012-10-24

申请号：EP11003760.3

申请日：2005-07-09

申请人： Promega Corporation

发明人： Darzins, Aldis ,  Encell, Lance P. ,  Johnson, Tonny ,  Klaubert, Dieter ,  Los, Georgyi V ,  McDougall, Mark ,  Wood, Keith V ,  Wood, Monika G ,  Zimprich, Chad

IPC分类号： G01N33/58 ,  C12N9/14 ,  C12Q1/34

CPC分类号： C12Q1/34 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变型水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。

公开(公告)号：EP2369006A1

公开(公告)日：2011-09-28

申请号：EP10075509.9

申请日：2004-01-30

申请人： Promega Corporation

发明人： Wood, Keith V. ,  Los, Georgyi V. ,  Bulleit, Robert F. ,  Klaubert, Dieter ,  McDougall, Mark ,  Zimprich, Chad

IPC分类号： C12Q1/34 ,  C12N9/14 ,  C12N9/86 ,  G01N33/58

CPC分类号： C12Q1/34 ,  C07C217/08 ,  C07C271/16 ,  C07D209/48 ,  C07D233/64 ,  C07D311/78 ,  C07D311/82 ,  C07D405/06 ,  C07D493/10 ,  C07D495/04 ,  C07F5/022 ,  C12N9/14 ,  C12N9/86 ,  C12Y308/01005 ,  G01N33/5005 ,  G01N33/58 ,  Y10S436/80

摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.