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    Covalent tethering of functional groups to proteins and substrates therefor
    2.
    发明公开
    Covalent tethering of functional groups to proteins and substrates therefor 有权
    官能团共价键合到蛋白质和底物上

    公开(公告)号：EP2395358A2

    公开(公告)日：2011-12-14

    申请号：EP11003760.3

    申请日：2005-07-09

    申请人： Promega Corporation

    发明人： Darzins, Aldis ,  Encell, Lance P. ,  Johnson, Tonny ,  Klaubert, Dieter ,  Los, Georgyi V ,  McDougall, Mark ,  Wood, Keith V ,  Wood, Monika G ,  Zimprich, Chad

    IPC分类号： G01N33/58 ,  C12N9/14 ,  C12Q1/34

    CPC分类号： C12Q1/34 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06

    摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

    摘要翻译： 提供了任选地与感兴趣的蛋白质融合的突变水解酶。 突变水解酶能够与底物形成结合的相应非突变（野生型）水解酶，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的用于水解酶的底物，以及使用突变水解酶和本发明底物的方法。 还提供了能够与底物和表达融合蛋白的细胞形成稳定键的融合蛋白。

    COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR
    6.
    发明公开
    COVALENT TETHERING OF FUNCTIONAL GROUPS TO PROTEINS AND SUBSTRATES THEREFOR 审中-公开
    功能基团与蛋白质及其底物的共价结合

    公开(公告)号：EP3179252A1

    公开(公告)日：2017-06-14

    申请号：EP17152698.1

    申请日：2005-07-29

    申请人： Promega Corporation

    发明人： Darzins, Aldis ,  Encell, Lance P ,  Johnson, Tonny ,  Klaubert, Dieter ,  Los, Georgyi V ,  McDougall, Mark ,  Wood, Keith V ,  Wood, Monika G ,  Zimprich, Chad

    IPC分类号： G01N33/58 ,  C12N9/14 ,  C12Q1/34

    CPC分类号： C12Q1/34 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06

    摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

    摘要翻译： 提供了任选地与感兴趣的蛋白质融合的突变水解酶。 突变水解酶能够与底物形成结合的相应非突变（野生型）水解酶，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的用于水解酶的底物，以及使用突变水解酶和本发明底物的方法。 还提供了能够与底物和表达融合蛋白的细胞形成稳定键的融合蛋白。

    Covalent tethering of functional groups to proteins and substrates therefor
    8.
    发明公开
    Covalent tethering of functional groups to proteins and substrates therefor 有权
    Kovales Anbinden von Funktionsgruppen an Proteine und Substratedafür

    公开(公告)号：EP2395358A3

    公开(公告)日：2012-10-24

    申请号：EP11003760.3

    申请日：2005-07-09

    申请人： Promega Corporation

    发明人： Darzins, Aldis ,  Encell, Lance P. ,  Johnson, Tonny ,  Klaubert, Dieter ,  Los, Georgyi V ,  McDougall, Mark ,  Wood, Keith V ,  Wood, Monika G ,  Zimprich, Chad

    IPC分类号： G01N33/58 ,  C12N9/14 ,  C12Q1/34

    CPC分类号： C12Q1/34 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y30/00 ,  C09B1/00 ,  C09B11/24 ,  C09B23/04 ,  C09B23/06

    摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

    摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变型水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。