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公开(公告)号：EP2395078B1
公开(公告)日：2017-05-10
申请号：EP11003761.1
申请日：2005-07-29
申请人： Promega Corporation
发明人： Darzins, Aldis , Encell, Lance P. , Johnson, Tonny , Klaubert, Dieter , Los, Georgyi V , McDougall, Mark , Wood, Keith V , Wood, Monika G , Zimbrich, Chad
CPC分类号： C12N9/14 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07K2299/00 , C12N9/0069 , C12Q1/26 , C12Q1/34 , C12Q1/66 , C12Y113/12005 , C12Y308/01005
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公开(公告)号：EP2341134B1
公开(公告)日：2014-08-27
申请号：EP10075510.7
申请日：2004-01-30
申请人： Promega Corporation
发明人： Wood, Keith V. , Los, Georgyi V. , Bulleit, Robert F. , Klaubert, Dieter , McDougall, Mark , Zimprich, Chad
CPC分类号： C12Q1/34 , C07C217/08 , C07C271/16 , C07D209/48 , C07D233/64 , C07D311/78 , C07D311/82 , C07D405/06 , C07D493/10 , C07D495/04 , C07F5/022 , C12N9/14 , C12N9/86 , C12Y308/01005 , G01N33/5005 , G01N33/58 , Y10S436/80
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公开(公告)号：EP2455457B1
公开(公告)日：2015-03-11
申请号：EP11005044.0
申请日：2004-01-30
申请人： PROMEGA CORPORATION
发明人： Wood, Keith, V. , Los, Georgyi, V , Bulleit, Robet, F. , Klaubert, Dieter , McDougall, Mark , Zimprich, Chard
CPC分类号： C12Q1/34 , C07C217/08 , C07C271/16 , C07D209/48 , C07D233/64 , C07D311/78 , C07D311/82 , C07D405/06 , C07D493/10 , C07D495/04 , C07F5/022 , C12N9/14 , C12N9/86 , C12Y308/01005 , G01N33/5005 , G01N33/58 , Y10S436/80
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公开(公告)号：EP2455457A3
公开(公告)日：2013-03-27
申请号：EP11005044.0
申请日：2004-01-30
申请人： PROMEGA CORPORATION
发明人： Wood, Keith, V. , Los, Georgyi, V , Bulleit, Robet, F. , Klaubert, Dieter , McDougall, Mark , Zimprich, Chard
CPC分类号： C12Q1/34 , C07C217/08 , C07C271/16 , C07D209/48 , C07D233/64 , C07D311/78 , C07D311/82 , C07D405/06 , C07D493/10 , C07D495/04 , C07F5/022 , C12N9/14 , C12N9/86 , C12Y308/01005 , G01N33/5005 , G01N33/58 , Y10S436/80
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
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公开(公告)号：EP2395358B1
公开(公告)日：2017-03-29
申请号：EP11003760.3
申请日：2005-07-29
申请人： Promega Corporation
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公开(公告)号：EP2455458A3
公开(公告)日：2013-04-24
申请号：EP11005045.7
申请日：2004-01-30
申请人： PROMEGA CORPORATION
发明人： Wood, Keith, V. , Los, Georgyi, V , Bulleit, Robert, F. , Klaubert, Dieter , McDougall, Mark , Zimprich, Chad
CPC分类号： C12Q1/34 , C07C217/08 , C07C271/16 , C07D209/48 , C07D233/64 , C07D311/78 , C07D311/82 , C07D405/06 , C07D493/10 , C07D495/04 , C07F5/022 , C12N9/14 , C12N9/86 , C12Y308/01005 , G01N33/5005 , G01N33/58 , Y10S436/80
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
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7.发明公开
公开(公告)号：EP2395358A2
公开(公告)日：2011-12-14
申请号：EP11003760.3
申请日：2005-07-09
申请人： Promega Corporation
发明人： Darzins, Aldis , Encell, Lance P. , Johnson, Tonny , Klaubert, Dieter , Los, Georgyi V , McDougall, Mark , Wood, Keith V , Wood, Monika G , Zimprich, Chad
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译： 提供了任选地与感兴趣的蛋白质融合的突变水解酶。 突变水解酶能够与底物形成结合的相应非突变（野生型）水解酶，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的用于水解酶的底物，以及使用突变水解酶和本发明底物的方法。 还提供了能够与底物和表达融合蛋白的细胞形成稳定键的融合蛋白。
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公开(公告)号：EP2341134A3
公开(公告)日：2011-08-31
申请号：EP10075510.7
申请日：2004-01-30
申请人： Promega Corporation
发明人： Wood, Keith V. , Los, Georgyi V. , Bulleit, Robert F. , Klaubert, Dieter , McDougall, Mark , Zimprich, Chad
CPC分类号： C12Q1/34 , C07C217/08 , C07C271/16 , C07D209/48 , C07D233/64 , C07D311/78 , C07D311/82 , C07D405/06 , C07D493/10 , C07D495/04 , C07F5/022 , C12N9/14 , C12N9/86 , C12Y308/01005 , G01N33/5005 , G01N33/58 , Y10S436/80
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译： 提供了任选地与感兴趣的蛋白质融合的突变水解酶。 突变水解酶能够与相应的非突变型（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定。 还提供了包含一个或多个官能团的用于水解酶的底物，以及使用突变水解酶和本发明底物的方法。 还提供了能够与底物和表达融合蛋白的细胞形成稳定键的融合蛋白。
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公开(公告)号：EP3660166A1
公开(公告)日：2020-06-03
申请号：EP19213528.3
申请日：2015-07-02
申请人： Promega Corporation
摘要： Provided herein are chelator constructs (e.g., nucleic acid, peptide, peptide nucleic acid, etc.) that sequester metal ions (e.g., Mg 2+ ) under a first set of conditions and fail to sequester or release sequestered metal ions under a second set of conditions. In particular, nucleic acid constructs are provided that sequester metal ions (e.g., Mg 2+ ) under conditions that favor secondary and tertiary structure formation and release or fail to sequester metal ions under conditions that disfavor the formation of such structures.
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公开(公告)号：EP3179252B1
公开(公告)日：2018-11-14
申请号：EP17152698.1
申请日：2005-07-29
申请人： Promega Corporation
发明人： Darzins, Aldis , Encell, Lance P , Johnson, Tonny , Klaubert, Dieter , Los, Georgyi V , McDougall, Mark , Wood, Keith V , Wood, Monika G , Zimprich, Chad
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
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