摘要:
The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes novel compounds and methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.
摘要:
The invention comprises suppressor oligonucleotides for reducing amplification of a non- target nucleic acid sequences; the method of designing and using such oligonucleotides, as well as kits and reaction mixtures.
摘要:
The present invention includes a method of allele-specific amplification, utilizing an allele- specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating at least one "G-clamp" nucleotide.
摘要:
The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.