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59 条记录 (耗时 0.016 秒)

    TRANSCRIPTION TERMINATOR AND USE THEREOF
    12.
    发明公开
    TRANSCRIPTION TERMINATOR AND USE THEREOF 审中-公开

    公开(公告)号：EP3384022A1

    公开(公告)日：2018-10-10

    申请号：EP16871350.1

    申请日：2016-11-29

    申请人： Gen9, Inc.

    发明人： SAAEM, Ishtiaq E.

    IPC分类号： C12N15/09 ,  C12N15/113 ,  C07H21/04

    CPC分类号： C12N15/113 ,  C12N15/63 ,  C12N2310/13

    摘要： Artificial transcription terminators and their use are provided herein. In one aspect, a non-naturally occurring nucleic acid sequence can comprise a Y-X-Z stem-loop, wherein: Y is a nucleotide sequence of 10 to 30 nucleotides in length; X is a nucleotide sequence of 3 to 12 nucleotides in length, each nucleotide therein not base pairing with any other nucleotide within X; and Z is a nucleotide sequence of 10 to 50 nucleotides in length and having at least 70% complementarity to Y.

    METHODS FOR NUCLEIC ACID ASSEMBLY
    14.
    发明公开
    METHODS FOR NUCLEIC ACID ASSEMBLY 审中-公开
    核酸组装方法

    公开(公告)号：EP3209777A1

    公开(公告)日：2017-08-30

    申请号：EP15852140.1

    申请日：2015-10-20

    申请人： Gen9, Inc.

    发明人： JACOBSON, Joseph ,  SAAEM, Ishtiaq E.

    IPC分类号： C12N15/10

    CPC分类号： C12P19/34 ,  C12N15/1031 ,  C12N15/1065

    摘要： Methods and compositions relate to the assembly of high fidelity nucleic acids. Specifically, nucleic acid molecules having a desired predetermined sequence can be assembled after failure to assemble in conventional assembly. Aspects of the disclosure relate to methods of assembling a target nucleic acid molecule having a desired or predetermined sequence.

    摘要翻译： 方法和组合物涉及高保真度核酸的组装。 具体而言，具有所需预定序列的核酸分子可以在未按照常规组装进行组装之后进行组装。 本公开的方面涉及组装具有期望或预定序列的靶核酸分子的方法。

    MICROARRAY SYNTHESIS AND ASSEMBLY OF GENE-LENGTH POLYNUCLEOTIDES
    18.
    发明公开
    MICROARRAY SYNTHESIS AND ASSEMBLY OF GENE-LENGTH POLYNUCLEOTIDES 审中-公开
    ON EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN EN

    公开(公告)号：EP3059321A1

    公开(公告)日：2016-08-24

    申请号：EP15189572.9

    申请日：2003-09-12

    申请人： Gen9, Inc.

    发明人： OLEINIKOV, Andrew, V.

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C07H21/04

    摘要： There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, "on device" assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

    摘要翻译： 公开了一种基于在微阵列平台上原位合成的多个较短的寡核苷酸的组装体外合成和组装长的基因长度多核苷酸的方法。 具体地，公开了在固相微阵列平台上原位合成寡核苷酸片段的方法，随后在由多个较短的寡核苷酸片段组成的较大多核苷酸的“器件”装配上。