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    COMPOSITIONS AND METHODS FOR HIGH FIDELITY ASSEMBLY OF NUCLEIC ACIDS
    4.
    发明公开
    COMPOSITIONS AND METHODS FOR HIGH FIDELITY ASSEMBLY OF NUCLEIC ACIDS 审中-公开

    公开(公告)号：EP3954770A1

    公开(公告)日：2022-02-16

    申请号：EP21177267.8

    申请日：2012-08-23

    申请人： Gen9, Inc.

    发明人： JACOBSON, Joseph ,  SCHINDLER, Daniel ,  LAWTON, Scott S.

    IPC分类号： C12N15/10 ,  C12N15/66 ,  C12P19/34 ,  C40B50/06

    摘要： Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid. In certain embodiments, the plurality of blunt-end double-stranded nucleic acid fragments can be provided by: releasing a plurality of oligonucleotides synthesized on a solid support; and synthesizing complementary strands of the plurality of oligonucleotides using a polymerase based reaction.

    METHODS AND APPARATUSES FOR CHIP-BASED DNA ERROR REDUCTION
    10.
    发明公开
    METHODS AND APPARATUSES FOR CHIP-BASED DNA ERROR REDUCTION 审中-公开

    公开(公告)号：EP3597771A1

    公开(公告)日：2020-01-22

    申请号：EP19174924.1

    申请日：2010-11-19

    申请人： Gen9, Inc.

    发明人： RAMU, Senthil ,  JACOBSON, Joseph ,  CHU, Larry Li-Yang

    IPC分类号： C12Q1/68

    摘要： Methods and apparatus relate to reduction of sequence errors generated during synthesis of nucleic acids on a microarray chip. The error reduction can include synthesis of complementary strands (to template strands), using a short universal primer complementary to the template strands and polymerase. Heteroduplex can be formed by melting and re-annealing complementary strands and template strands. The heteroduplexes containing a mismatch can be recognized and cleaved by a mismatch endonuclease. The mismatch-containing cleaved heteroduplexes can be removed from the microarray chip using a global buffer exchange. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.