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公开(公告)号：EP2947157A4
公开(公告)日：2016-08-03
申请号：EP14740752
申请日：2014-01-16
IPC分类号： C12Q1/68 , C12N5/0735 , C12N5/074 , C12N5/0775 , G01N33/48 , G01N33/53 , G01N37/00
CPC分类号： C12Q1/6888 , C12Q1/6809 , C12Q2600/154 , G06F19/20 , C12Q2523/125 , C12Q2537/165 , C12Q2539/115 , C12Q2565/501
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12.发明公开DEVICE AND METHOD FOR PRETREATING SEQUENCER 审中-公开VORRICHTUNG UND VERFAHREN ZUR VORBEHANDLUNG EINES序列号
公开(公告)号：EP3000896A1
公开(公告)日：2016-03-30
申请号：EP14800557.2
申请日：2014-05-21
发明人： TAJIMA, Hideji
摘要： With respect to a sequencer pretreatment device and a method thereof, an object is to provide reliable pretreatment for analyzing a large-scale base sequence.
Using is a sequencer pretreatment device including a suction and discharge mechanism, a nozzle head having a nozzle for detachably mounting a dispensing tip, a container group for accommodating various liquids including magnetic particle suspension, a moving mechanism for causing the nozzle relative movement with respect to the container group, and a magnetic unit capable of exerting a magnetic field to the mounted dispensing tip, the method is configured to include an extraction step of mixing a sample, extraction reagent solution, and magnetic particle suspension in the container group and extracting nucleic acid, a fragmentation producing step of fragmentating the nucleic acid by mixing with fragmentation solution accommodated in the container group and producing, from the fragmentated nucleic acid, a fragment of a base sequence having the number of bases corresponding to a sequencer using magnetic particle suspension using the sequencer pretreatment device, and an amplification pretreatment step of dispensing a predetermined volume of solution containing the produced fragment into the reaction vessel using the sequencer pretreatment device.摘要翻译： 对于定序器预处理装置及其方法，目的是提供用于分析大规模碱基序列的可靠的预处理。 使用的是包括抽吸和排出机构的定序器预处理装置，具有用于可拆卸地安装分配尖端的喷嘴的喷嘴头，用于容纳包括磁性颗粒悬浮液在内的各种液体的容器组，用于使喷嘴相对于 所述容器组和能够向所述安装的分配尖端施加磁场的磁性单元，所述方法被配置为包括将样品，提取试剂溶液和磁性颗粒悬浮液混合在所述容器组中并提取核酸的提取步骤 ，通过与容纳在容器组中的碎裂溶液混合并与片段化的核酸一起使用磁性粒子悬浮液，使用磁性粒子悬浮液从片段化的核酸产生具有与定序器对应的碱基序列数的碱基序列片段， 定序器预处理装置，以及放大预处理装置 处理步骤，使用定序器预处理装置将含有所产生的片段的预定体积的溶液分配到反应容器中。
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13.发明公开MAGNETIC REAGENT, MAGNETIC REAGENT KIT, METHOD FOR TREATING MAGNETIC CARRIERS AND TREATMENT DEVICE THEREFOR 审中-公开磁性试剂，具有磁性试剂套件，一种用于处理磁性载体和处理装置THEREFOR
公开(公告)号：EP2500101A4
公开(公告)日：2015-12-16
申请号：EP10830035
申请日：2010-11-12
发明人： TAJIMA HIDEJI
IPC分类号： B03C1/00 , B01J20/28 , B03C1/015 , B03C1/28 , G01N33/543 , G01N33/553
CPC分类号： B03C1/015 , B03C1/288 , B03C2201/18 , B03C2201/26 , G01N33/54326
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公开(公告)号：EP2947157A1
公开(公告)日：2015-11-25
申请号：EP14740752.2
申请日：2014-01-16
IPC分类号： C12Q1/68 , G01N33/48 , G01N33/53 , G01N37/00 , C12N5/0735 , C12N5/074 , C12N5/0775
CPC分类号： C12Q1/6888 , C12Q1/6809 , C12Q2600/154 , G06F19/20 , C12Q2523/125 , C12Q2537/165 , C12Q2539/115 , C12Q2565/501
摘要： It is an object of the present invention to provide a method for analyzing, in detail, the epigenetic state of stem cells.
The present invention provides a method for identifying stem cells, which comprises analyzing the patterns of methylation and demethylation of chromosomal DNA extracted from test stem cells, and correlating the analyzed patterns with the properties of the test stem cells.-
15.发明公开AUTOMATED NUCLEIC ACID PROCESSOR AND AUTOMATED NUCLEIC ACID PROCESSING METHOD USING MULTI FUNCTION DISPENSING UNIT 审中-公开自动化核酸处理器和自动化核酸过程中利用多功能输出单元
公开(公告)号：EP2628786A4
公开(公告)日：2015-09-30
申请号：EP11832627
申请日：2011-10-14
发明人： TAJIMA HIDEJI
CPC分类号： C12Q1/686 , B01L3/0217 , B01L3/0275 , B01L3/523 , B01L3/527 , B01L7/52 , B01L2200/026 , B01L2200/0689 , B01L2200/142 , B01L2300/024 , B01L2300/044 , B01L2300/0654 , B01L2300/0672 , B01L2300/0829 , B01L2300/1822 , B01L2300/1888 , C12Q1/6806 , C12Q2563/143 , C12Q2563/149 , C12Q2565/629 , G01N21/75 , G01N35/00732 , G01N35/04 , G01N35/1065 , G01N2035/00742 , G01N2035/00821 , G01N2035/00851 , G01N2035/0403 , G01N2035/103 , G01N2035/1048 , G01N2035/1055 , G01N2035/1062
摘要： In relation to an automated nucleic acid processor and an automated nucleic acid processing method using a multi function dispensing unit, processing involving extraction and amplification of the nucleic acid, can be consistently, quickly and efficiently conducted at a low cost with the use of a multi function dispensing unit, while saving user's trouble without expanding the scale of the device. The multi function dispensing unit includes: a nozzle head provided with a suction-discharge mechanism and nozzles detachably provided with dispensing tips; a container group having, at the very least housing parts for liquids and reaction containers for housing an amplification solution; a transfer mechanism that makes an interval between the nozzles and the container group relatively movable; a temperature controller whereby temperature control of the interior of the reaction vessels is possible; sealing liquids and/or sealing lids that are transportable to the reaction vessels using the nozzles, and which make the amplification solutions housed in the reaction vessels sealable within the reaction vessels; and a sealing control part that controls the suction-discharge mechanism or the transfer mechanism, such that the sealing liquid and/or the sealing lids seal the amplification solution within the reaction vessels when the housing of the amplification solution in the reaction vessels is completed.
摘要翻译： 相对于上自动化核酸处理器和自动核酸处理方法使用多功能分配单元，处理涉及提取和核酸扩增，可以是一致，快速，以低成本与使用多的有效地进行 功能分配单元，同时节省了用户的麻烦不扩大装置规模。 多功能分配单元包括：设置有抽吸排出机构和可拆卸地设置有分配尖端喷嘴的喷嘴头; 具有容器组，至少是，用于液体和反应容器的扩增溶液壳体的壳体部分; 转移机构做了喷嘴和容器组可相对移动之间的时间间隔的品牌; 温度控制器，由此反应容器的内部的温度控制是可能的; 密封液体和/或密封的盖确实是可运输的，以使用所述的喷嘴的反应容器，并且使在密封的反应容器内的反应容器容纳在所述放大溶液; 和密封控制部确实控制吸入排出机构或转移机构，检查并当完成在反应容器中的扩增溶液的壳体中的密封液和/或密封的盖子密封该反应容器内的扩增溶液。
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16.发明公开ANALYSIS METHOD AND ANALYSIS KIT FOR SIMULTANEOUSLY DETECTING OR QUANTITATING MULTIPLE TYPES OF TARGET SUBSTRANCES 审中-公开分析方法分析系统同时检测和目标物质的几种量化
公开(公告)号：EP2910930A1
公开(公告)日：2015-08-26
申请号：EP13849432.3
申请日：2013-10-21
IPC分类号： G01N21/75 , G01N33/543
CPC分类号： G01N33/54306 , G01N21/6428 , G01N21/76 , G01N33/54313 , G01N2021/7793 , G01N2333/59
摘要： Signal lights from probe beads are localized for simultaneous detection or quantification of plural species of target substances.
The present invention provides an analysis method for simultaneously detecting or quantifying plural species of target substances by measuring the light emission from plural carriers within a single container from outside of the container, these carriers comprising a plurality of substances that are respectively immobilized thereon for capturing the plural species of target substances, wherein the light emission from a desired carrier is selectively received by measuring the emission from the carriers through an optical filter. The present invention also provides an analysis kit for simultaneously detecting or quantifying plural species of target substances by measuring the light emission from plural carriers within a single container from outside of the container, these carriers comprising a plurality of substances that are respectively immobilized thereon for capturing the plural species of target substances, which comprises the plural carriers and an optical filter for selectively receiving the emission from a desired carrier.摘要翻译： 从探针珠信号光被本地化为同时检测或目标物质的多个种类的定量。 对本发明的分析方法提供了用于同时检测或通过测量从所述容器的外部的单个容器内的多个载波上的光发射定量靶物质的多个物种，本文的载体包括多种物质的多元并分别固定在其上，用于捕获 目标物质的多个物种，从所期望的载体worin发光选择性地通过到光学滤光器测量从载流子的发射接收。 因此，本发明的分析试剂盒提供用于同时检测或通过测量从所述容器的外部的单个容器内的多个载波上的光发射定量靶物质的多个物种，本文的载体包括多种物质的多元并分别固定在其上，用于捕获 多个种类的目标物质的方法，其包括的多个载波并选择性光滤波器，用于从期望的载波接收所述发射。
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公开(公告)号：EP2000808A4
公开(公告)日：2015-07-08
申请号：EP07740037
申请日：2007-03-28
发明人： TAJIMA HIDEJI
CPC分类号： G01N1/28 , B01L3/5085 , B01L9/54 , B01L2200/141 , B01L2300/0829 , G01N35/1074 , Y10T436/2575
摘要： An object is to provide a micro plate treating device and micro plate treating method capable of handling a larger number of kinds of solution or suspensions or a larger volume of solutions or suspensions per work area by use of a normalized micro plate without enlarging the scale of the device. The micro plate treating device comprises a micro plate having wells arranged in a matrix form, one or two or more nozzle heads each having nozzles capable of sucking and discharging a fluid and arranged in a matrix form, and moving means capable of moving relatively an interval between the micro plate and the nozzle heads, wherein tips of all the nozzles are configured to be permitted to be simultaneously inserted into some of the wells in the micro plate, at least one of the row interval or the column interval of the nozzles is set to a natural number multiple of the row interval or the column interval of the corresponding wells, the natural number being two or more, and at least one of the row number or the column number of all the corresponding nozzles is one to the natural number of the row number or the column number of the wells.
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18.发明公开LIGHT MEASUREMENT APPARATUS FOR REACTION VESSEL, AND LIGHT MEASUREMENT METHOD 有权LICHTMESSVORRICHTUNGFÜRREAKTIONSGEFÄSSUND LICHTMESSVERFAHREN
公开(公告)号：EP2876432A1
公开(公告)日：2015-05-27
申请号：EP13819199.4
申请日：2013-07-17
摘要： The invention relates to a light measurement apparatus for a reaction vessel, and a light measurement method, whereby the optical state on the inside of a reaction vessel can be measured efficiently and reliably.
The light measurement apparatus includes: a plurality of linking portions to which is provided front ends of light guide portions that optically connect with the interior of the reaction vessel that is linkable with reaction vessels; a connecting end arranging body that has an arranging surface that arranges and supports along a predetermined path, a plurality of connecting ends to which is provided back ends of the light guide portions, in which the front ends thereof are provided to the linking portions, the connecting ends are provided corresponding to the respective linking portions; a measurement device that has measuring ends that are successively optically connectable with the respective connecting ends, and in which light based on the optical state is receivable by means of optical connections between the connecting ends and the measuring ends; and a light guide switching mechanism that successively optically connects the respective connecting ends and the respective measuring ends.摘要翻译： 本发明涉及一种用于反应容器的光测量装置和光测量方法，由此能够有效且可靠地测量反应容器内部的光学状态。 光测量装置包括：多个连接部分，其设置在与反应容器可连接的反应容器的内部光学连接的导光部分的前端; 具有沿预定路径布置和支撑的布置表面的连接端布置体，多个连接端设置在其前端设置到连接部分的导光部的后端， 对应于相应的连接部分设置连接端; 测量装置，其具有可以与各个连接端连续光学连接的测量端，并且其中基于光学状态的光可以通过连接端和测量端之间的光学连接而被接收; 以及导光切换机构，其连续地连接各个连接端和相应的测量端。
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19.发明公开Biological material fixed carrier enclosing tip, biological material fixed carrier treatment apparatus, and treatment method thereof 审中-公开生物材料包封它们Biomaterialträgers的前端处理装置及处理方法
公开(公告)号：EP2620778A3
公开(公告)日：2014-08-20
申请号：EP13165049.1
申请日：2005-12-12
发明人： TAJIMA, Hideji
CPC分类号： B01L3/502 , B01L3/0275 , B01L2200/0631 , B01L2200/0647 , B01L2300/0609 , B01L2300/0636 , B01L2300/12 , G01N35/10 , G01N2035/1053
摘要： A biological material fixed carrier treatment apparatus (10) comprising a nozzle head (84) that has one or a plurality of consecutive nozzles (117) that perform suction and discharge of gas; a suction and discharge mechanism that performs suction and discharge of gas via the nozzles; one or two or more biological material fixed carrier enclosing tips (35) that are installed or are installable to the nozzles, in which a fixed carrier, to which biological material is fixable or is fixed, is enclosed within a narrow tube that possesses an opening, through which fluid inflow and outflow is possible by means of said suction and discharge of gas, and that is narrower than said nozzle; a stage to which a liquid storage section group, in which a variety of liquids are stored or are storable, is provided; a movement device that relatively moves said nozzle head with respect to said liquid storage section group; and a control section that is adapted to controls the amount, the speed, the frequency, the time, or the position of the suction and discharge of said nozzles based on; the structure of said biological material fixed carrier enclosing tip, the material conditions comprising the type of biological material that is fixed to the carrier or is present within the fluid, the concentration, the amount of liquid, and the coordinate position containing the storage position of the liquid, and the processing contents, and adapted to control the temperature control based on the processing contents, wherein a temperature raising and lowering body (71) that raises and lowers the temperature as a result of a signal from the exterior is provided in the vicinity of , making contact with, or able to be in the vicinity of or making contact with, the outside of said narrow tube of said biological material fixed carrier enclosing tip.
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20.发明公开REACTION CONTAINER AND METHOD FOR PRODUCING SAME 审中-公开REAKTIONSBEHÄLTERUND HERSTELLUNGSVERFAHRENDAFÜR
公开(公告)号：EP2679664A1
公开(公告)日：2014-01-01
申请号：EP11859097.5
申请日：2011-08-19
发明人： TAJIMA Hideji
CPC分类号： B65D7/12 , B01L3/50855 , B01L3/523 , B01L3/5635 , B01L7/52 , B01L2200/028 , B01L2300/044 , B01L2300/0832 , B01L2300/1805 , B01L2300/1822 , G01N2201/0826
摘要： The invention relates to a reaction container and a method for producing the reaction container, and provides a low-cost reaction container which is capable of consistently, quickly, and efficiently performing a process that includes a reaction such as extraction or amplification of a nucleic acid, while saving user's trouble without increasing the scale of the device.
The reaction container is configured to comprise: one or two or more housing parts for reactions comprising: a narrow-mouthed piping part in which a reaction reagent or a portion thereof is housed or is housable; a wide-mouthed piping part that is communicated with the narrow-mouthed piping part and provided on an upper side of the narrow-mouthed piping part, and has an aperture that is wider than an aperture of the narrow-mouthed piping part; and a punchable film provided such that it partitions an interval between the wide-mouthed piping part and the narrow-mouthed piping part.摘要翻译： 本发明涉及反应容器和反应容器的制造方法，提供一种低成本的反应容器，其能够一贯，快速，有效地进行包括反应的过程，例如提取或扩增核酸 同时在不增加设备规模的同时节省用户的麻烦。 反应容器被构造成包括：用于反应的一个或两个或更多个壳体部分，包括：其中容纳有反应试剂或其一部分或可容纳的窄口管道部分; 与狭窄的管道部分连通并设置在狭窄的管道部分的上侧的宽口管部分，并且具有比狭窄的管道部分的孔宽的孔; 以及设置成使得其分开宽口管道部分和窄口管道部分之间的间隔的可冲压膜。
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