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35 条记录 (耗时 0.018 秒)

    NUCLEIC ACID HYBRIDIZATION ASSAY
    11.
    发明授权
    NUCLEIC ACID HYBRIDIZATION ASSAY 失效
    核酸混合测定

    公开(公告)号：EP0165313B1

    公开(公告)日：1989-11-15

    申请号：EP85900525.8

    申请日：1984-12-07

    申请人： HRI RESEARCH, INC.

    发明人： YABUSAKI, Kenichi, Ken ,  ISAACS, Stephen, T. ,  GAMPER, Howard, B., Jr.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6816 ,  C12Q2563/101 ,  C12Q2523/101

    摘要： Novel methods and reagents for determining the presence of specific nucleic acid base sequences by employing crosslinking reactions of unique molecules capable of forming covalent bonds which are bonded with various labels or ligands for amplication. Single stranded nucleic acid probes are employed which contain complementary base sequences to nucleic acid target molecules. By first hybridizing and then forming covalent bonds between the probe and target the amount of label in the crosslinked hybrid can be measured as an extremely sensitive method for assaying for specific nucleic acid sequences.

    Detection of nucleic acids by nuclease-catalyzed product formation
    14.
    发明公开
    Detection of nucleic acids by nuclease-catalyzed product formation 失效
    Nachweis vonNukleinsäurendurch Nuklease-katalysierte Produktbildung

    公开(公告)号：EP1260593A2

    公开(公告)日：2002-11-27

    申请号：EP02008466.1

    申请日：1995-12-22

    申请人： DADE BEHRING INC.

    发明人： Ullman, Edwin, F. ,  Western, Linda, M. ,  Rose, Samuel, J.

    IPC分类号： C12Q1/68 ,  C12Q1/44

    CPC分类号： C12Q1/689 ,  C12Q1/682 ,  C12Q1/6823 ,  C12Q2521/319 ,  C12Q2537/149 ,  C12Q2521/301 ,  C12Q2563/101

    摘要： A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5'-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5'-nuclease to cleave the oligonucleotide to provide (i) a first fragment that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3' of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte. The method has particular application to the detection of a polynucleotide analyte such as DNA. Kits for conducting methods in accordance with the invention are also disclosed.

    摘要翻译： 公开了一种修饰寡核苷酸的方法，该方法可用于检测多核苷酸分析物。 在等温条件下，在5'-核酸酶的存在下，寡核苷酸与多核苷酸例如多核苷酸分析物可逆地杂交。 多核苷酸分析物用作识别元件，使得5'-核酸酶能够切割寡核苷酸以提供（i）与多核苷酸分析物基本上不可杂交的第一片段和（ii）位于3'端的第3个片段 第一片段（在完整寡核苷酸中）并且与多核苷酸分析物基本上可杂交。 相对于多核苷酸分析物的摩尔量，获得至少100倍摩尔过量的第一片段和/或第二片段。 检测到第一片段和/或第二片段的存在，其存在表明多核苷酸分析物的存在。 该方法特别适用于多核苷酸分析物如DNA的检测。 还公开了用于根据本发明的方法的套件。

    Oligonucleotides labeled with stable isotopes and a method for detecting the same
    15.
    发明公开
    Oligonucleotides labeled with stable isotopes and a method for detecting the same 有权
    Mit稳定同位素标记寡核苷酸和方法测定

    公开(公告)号：EP1041145A2

    公开(公告)日：2000-10-04

    申请号：EP00106939.2

    申请日：2000-03-31

    申请人： NIPPON SANSO CORPORATION

    发明人： Kawai, Gota ,  Wada, Akira ,  Takaku, Hiroshi

    IPC分类号： C12N15/11 ,  C12Q1/68

    CPC分类号： C12N15/113 ,  C12N2310/3125 ,  C12N2310/315 ,  C12N2310/3181 ,  C12N2310/3517 ,  C12Q1/68 ,  Y10T436/24 ,  C12Q2565/633 ,  C12Q2563/101

    摘要： Antisense oligonucleotide sequences which enable the measurement of the distribution and structure of antisense oligonucleotide drugs in the body, with lapse of time, and a method of detecting these sequences are provided. The antisense chains have a natural or non-natural nucleotide or peptide nucleic acid as a structure unit in which carbon atoms and nitrogen atoms are substituted by 13 C and 15 N, respectively, and the antisense chains can be detected by nuclear magnet resonance spectrometry (NMR) such as 15 N- 1 H or 13 C- 1 H heteronuclide multiple quantum coherence spectrometry.

    摘要翻译： 提供能够测量体内反义寡核苷酸药物在时间上的分布和结构的反义寡核苷酸序列，以及检测这些序列的方法。 反义链具有天然或非天然核苷酸或肽核酸作为其中碳原子和氮原子分别被13 C和15 N取代的结构单元，反义链可以通过核检测 磁共振光谱（NMR）如15 N-1 H或13 C-H异质核多重量子相干光谱法。

    ENHANCED CHROMATOGRAPHY USING MULTIPHOTON DETECTION
    16.
    发明公开
    ENHANCED CHROMATOGRAPHY USING MULTIPHOTON DETECTION 失效
    增强型离子色谱法MULTI PHOTO NACHWEISS

    公开(公告)号：EP0912899A1

    公开(公告)日：1999-05-06

    申请号：EP97933397.0

    申请日：1997-07-11

    申请人： Biotraces, Inc.

    发明人： DRUKIER, Andrzej, K. ,  BIELSKI, Roman

    IPC分类号： C12Q1 ,  G01N33 ,  G01T1 ,  G01T7

    CPC分类号： C12Q1/6816 ,  G01N30/74 ,  G01N33/60 ,  G01N2030/77 ,  G01T1/204 ,  G01T1/208 ,  G01T7/08 ,  Y10S435/807 ,  Y10S436/804 ,  Y10T436/115831 ,  Y10T436/13 ,  C12Q2565/137 ,  C12Q2563/101 ,  C12Q2565/125

    摘要： A method for detecting an analyte of interest present in a mixture at an ultralow concentration includes selecting a radioactive derivatizing agent comprising a bound multiphoton-emitting radioisotope and a moiety reactive with the analyte of interest, the radioisotope moiety being bound to the derivatizing agent by a bond that is stable under the conditions employed in the other steps of the method, derivatizing the analyte of interest with the derivatizing agent, separating the analyte of interest from other components of the mixture by chromatography, and detecting the analyte of interest using multiphoton detection. The derivatizing step may be performed before or after fractionation. A radiophore for multiphoton emission enhanced chromatography has a first moiety bound to a multiphoton-emitting radioisotope, and a second moiety that is reactive with a functional group of an analyte of interest. The first moiety may be a benzene group having an alkoxy, amino, or thiol group, bound to a halogen radioisotope; a complexing agent able to form a stable complex with a metal radioisotope such as a lanthanide or heavy metal.

    DETECTION OF NUCLEIC ACIDS BY TARGET-CATALYZED PRODUCT FORMATION
    17.
    发明公开
    DETECTION OF NUCLEIC ACIDS BY TARGET-CATALYZED PRODUCT FORMATION 失效
    检测NUCLEIC BY ZIELKATALYSIERTE的产品培训

    公开(公告)号：EP0842294A2

    公开(公告)日：1998-05-20

    申请号：EP95944648.0

    申请日：1995-12-22

    申请人： Behring Diagnostics GmbH

    发明人： ULLMAN, Edwin, F. ,  WESTERN, Linda, M. ,  ROSE, Samuel, J.

    IPC分类号： C12Q1

    CPC分类号： C12Q1/689 ,  C12Q1/682 ,  C12Q1/6823 ,  C12Q2521/319 ,  C12Q2537/149 ,  C12Q2521/301 ,  C12Q2563/101

    摘要： A method is disclosed for modifying an oligonucleotide, which method has application to the detection of a polynucleotide analyte. An oligonucleotide is reversibly hybridized with a polynucleotide, for example, a polynucleotide analyte, in the presence of a 5'-nuclease under isothermal conditions. The polynucleotide analyte serves as a recognition element to enable a 5'-nuclease to cleave the oligonucleotide to provide (i) a first fragment that is substantially non-hybridizable to the polynucleotide analyte and (ii) a second fragment that lies 3' of the first fragment (in the intact oligonucleotide) and is substantially hybridizable to the polynucleotide analyte. At least a 100-fold molar excess of the first fragment and/or the second fragment are obtained relative to the molar amount of the polynucleotide analyte. The presence of the first fragment and/or the second fragment is detected, the presence thereof indicating the presence of the polynucleotide analyte. The method has particular application to the detection of a polynucleotide analyte such as DNA. Kits for conducting methods in accordance with the present invention are also disclosed.

    THERMOSTABLE POLYMERASE DNA SEQUENCING REACTION CONCENTRATE
    18.
    发明授权
    THERMOSTABLE POLYMERASE DNA SEQUENCING REACTION CONCENTRATE 失效
    可热聚合物DNA序列反应浓度

    公开(公告)号：EP0455744B1

    公开(公告)日：1993-08-18

    申请号：EP90903572.7

    申请日：1990-01-31

    申请人： EASTMAN KODAK COMPANY

    发明人： VIZARD, Douglas, Lincoln ,  HADMAN, Martin

    IPC分类号： C12Q1/68 ,  C12N9/12

    CPC分类号： C12Q1/701 ,  C12Q1/6869 ,  C12Q2563/101 ,  C12Q2535/101 ,  C12Q2521/101

    摘要： The present invention provides a nucleotide sequencing reaction concentrate comprising: a) sufficient thermostable polymerase to provide from 100 to 500 I.U./mL; b) from 0.3 to 30 mM of a reducing agent selected from dithiothreitol and β-mercaptoethanol; c) sufficient phosphate buffer to maintain a pH of about 7.5; d) at least 40% glycerol; e) from 15 to 70 νM dGTP or daGTP; f) from 15 to 150 νM dATP; g) from 15 to 150 νM dTTP; h) from 10 to 18 νM dCTP; and i) one member selected from the group consisting of: i) sufficient ddGTP to provide a ddGTP to dGTP or daGTP ratio of about 5; ii) sufficient ddATP to form a ddATP to dATP ratio of about 33; iii) ddTTP in a sufficient amount to form a ddTTP to dTTP ratio of about 28; and iv) ddCTP in a sufficient amount to form a ddCTP to dCTP ratio of about 22.

    THERMOSTABLE POLYMERASE DNA SEQUENCING REACTION CONCENTRATE
    19.
    发明公开
    THERMOSTABLE POLYMERASE DNA SEQUENCING REACTION CONCENTRATE 失效
    反应精矿带热稳定DNA聚合酶的DNA测序。

    公开(公告)号：EP0455744A1

    公开(公告)日：1991-11-13

    申请号：EP90903572.0

    申请日：1990-01-31

    申请人： EASTMAN KODAK COMPANY

    发明人： VIZARD, Douglas, Lincoln ,  HADMAN, Martin

    IPC分类号： C12Q1

    CPC分类号： C12Q1/701 ,  C12Q1/6869 ,  C12Q2563/101 ,  C12Q2535/101 ,  C12Q2521/101

    摘要： La présente invention concerne un concentré de réaction de mise en séquence de nucléotide comprenant: (a) une polymérase suffisante et thermostable pour obtenir de 100 à 500 IU/mL; (b) de 0,3 à 30 mM d'un agent réducteur sélectionné entre le dithiothréitol et beta-mercaptoéthanol; (c) un tampon de phosphate en quantité suffisante pour maintenir un pH d'environ 7,5; (d) au moins 40 % de glycérol; (e) de 15 à 70 muM dGTP ou daGTP; (f) de 15 à 150 muM dATP; (g) de 15 à 150 muM dTTP; (h) de 10 à 18 muM dCTP; et (i) un élément sélectionné dans le groupe constitué de: (i) ddGTP en quantité suffisante pour obtenir un rapport entre ddGTP et dGTP or daGTP d'environ 5; (ii) ddATP en quantité suffisante pour former un rapport entre ddATP et dATP d'environ 33; (iii) ddTTP en quantité suffisante pour obtenir un rapport entre ddTTP et dTTP d'environ 28; et (iv) ddCTP en quantité suffisante pour obtenir un rapport entre ddCTP et dCTP d'environ 22.