利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

105 条记录 (耗时 0.017 秒)

    METHOD FOR DETECTING A BIOLOGICALLY ACTIVE SUBSTANCE IN A LIQUID TO BE ANALYSED AND DEVICE FOR REALISING THE SAME
    13.
    发明公开
    METHOD FOR DETECTING A BIOLOGICALLY ACTIVE SUBSTANCE IN A LIQUID TO BE ANALYSED AND DEVICE FOR REALISING THE SAME 失效
    这种用于测定生物活性物质在一方法将待分析液体和该装置体现

    公开(公告)号：EP0881485A1

    公开(公告)日：1998-12-02

    申请号：EP97913572.0

    申请日：1997-11-14

    申请人： INSTITUT MOLEKULYARNOI BIOLOGII IM. V.A.ENGELGARDTA ROSSIISKOI AKADEMII NAUK ,  Institut Spektroskopii Rossiiskoi Akademii Nauk

    发明人： EVDOKIMOV, Jury Mikhailovich ,  SKURIDIN, Sergei Gennadievich ,  CHERNUKHA, Boris Alexandrovich ,  MIKHAILOV, Evgeny Leonidovich ,  KOMPANETS, Oleg Nikolaevich ,  ROMANOV, Sergei Nikolaevich ,  KOLOSOV, Vladimir Vasilievich

    IPC分类号： G01N21/19 ,  C07H21/00

    CPC分类号： G01N21/314 ,  G01N21/19

    摘要： The present invention allows quickly, simply, precisely and with high sensitivity to determine the presence and concentration of any biologically active substances capable to interact with linear double-stranded DNA molecules in any liquids including biological liquids. The essence of the offered method consists that the lyotropic liquid-crystalline cholesteric DNA dispersion is formed in an aqueous-salted solution of a polymer, neutral in respect to DNA, of the linear double-stranded DNA molecules of a low molecular mass immediately before mixing with an analyzed liquid containing the determined substance, thereat the liquid for the analysis is prepared by mixing with the specified polymer under conditions, when the optical properties of the lyotropic liquid-crystalline DNA dispersion are not broken, then through the sample obtained as a result of mixing of the prepared analysed liquid with the liquid-crystalline DNA dispersion the circular-polarized light is passed, and the optical signal is registered at two wavelengths, one of them is in the region of the DNA absorption, and another one is in the region of absorption of the biologically active substance, then the ratio between these two signals is calculated, and concentration of the biologically active substance is determined on this ratio using the calibration curve. The offered device contains a wavelength selector (2) having an electrodynamic driver (22) of a positional type and at least one optical element (26, 27, 28) fixed on a motor (33) shaft (35) of the specified driver (22).

    摘要翻译： 本发明允许快速，简单，精确，并用确定性矿的高灵敏度能够与在包括生物液体的任何液体线性双链DNA分子相互作用的任何生物活性物质的存在和浓度。 待售方法besteht的本质做了易溶液晶胆甾型DNA分散形成在混合之前wässrige盐渍的聚合物的溶液，中性在相对于DNA，低分子量的线性双链DNA分子的立即 用在分析液体含确定性开采的物质，在该处用于分析的液体通过的条件下指定的聚合物，当该溶致液晶DNA分散体的光学特性没有破损混合来制备，然后通过样品获得作为结果 与液晶DNA分散体中来制备分析液体的混合的圆形偏振光通过，并将该光信号被登记在两个波长，它们中的一个是在DNA吸收的区域中，而另一个是在 生物活性物质的吸收区域中，则本文之间的比例的两种信号进行计算，并且该生物的浓度 活性物质确定性开采上使用校正曲线这个比率。 所提供的装置包含指定的驱动器的固定在马达（33），轴（35）的波长选择器（2），其具有在一个位置类型和至少一个光学元件（26，27，28）的电动驱动器（22）（ 22）。

    METHOD AND DICHROGRAPH FOR MEASUREMENT OF CIRCULAR DICHROISM, OPTICAL ROTATION AND ABSORPTION SPECTRA
    14.
    发明授权
    METHOD AND DICHROGRAPH FOR MEASUREMENT OF CIRCULAR DICHROISM, OPTICAL ROTATION AND ABSORPTION SPECTRA 失效
    方法和DICHROGRAPH用于测量ZIRKULAREM二色性旋光性和ABSORTIONSSPEKTRUM

    公开(公告)号：EP0681692B1

    公开(公告)日：1998-09-09

    申请号：EP95900603.2

    申请日：1994-11-25

    申请人： ROKOS & CO, LTD.

    发明人： ROKOS, Jiri

    IPC分类号： G01N21/19

    CPC分类号： G01N21/19

    摘要： The dichrograph and accompanying method are usable for parallel measurement of circular dichroism according to the first harmonic of photoelectric current of the detector, optical rotation acccording to the second harmonic and transparency according to the null harmonic. It contains the switch (3) of ray, from which alternatively exit two parallel rays - measurement ray (4) and reference ray (5), going through one mutual modulator of elipticity (6), achromatic phase element (9) which is during calibration of circular unit situated in the reference ray (5). Moreover it contains two analyzers - the measurement analyzer (8) in the measurement ray (4) behind the couvette (7) and the reference analyzer (10) in the reference ray (5). The measurement analyzer (8) has the optical axis turned for 45 DEG same as the modulator (6). The reference analyzer (10) is during measurement turned for unit angle, e.g. for 1 DEG , to the measurement analyzer (8).