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    METHOD AND REAGENT FOR QUANTIFYING CHOLESTEROL IN HIGH DENSITY LIPOPROTEIN 3
    21.
    发明公开
    METHOD AND REAGENT FOR QUANTIFYING CHOLESTEROL IN HIGH DENSITY LIPOPROTEIN 3 审中-公开
    VERFAHREN ZUR QUANTIFIZIERUNG DER CHOLESTERINMENGE IN HOCHDICHTEM LIPOPROTEIN 3

    公开(公告)号：EP3026120A4

    公开(公告)日：2017-02-22

    申请号：EP14829002

    申请日：2014-07-24

    申请人： DENKA SEIKEN KK

    发明人： SATO NORIYUKI ,  ITOH YASUKI

    IPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/34 ,  G01N33/48 ,  G01N33/50 ,  G01N33/92

    CPC分类号： G01N33/92 ,  C07C43/20 ,  C07C43/215 ,  C12Q1/60 ,  G01N2405/00

    摘要： Disclosed are a method and a reagent for quantifying HDL3 in a test sample without requiring laborious operations. The method for quantifying cholesterol in high-density lipoprotein 3 comprises reacting a test sample with one or more surfactants which react specifically with high-density lipoprotein 3, and quantifying cholesterol. When one surfactant is used, the surfactant is one selected from the group consisting of polyoxyethylene polycyclic phenyl ethers having an HLB of 12.5 to 15. When two or more surfactants are used, at least one of the surfactants is at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ethers, and the two or more surfactants are combined so as to provide the total HLB of 12.5 to 15 of the combined surfactants.

    摘要翻译： 公开了一种用于在测试样品中量化HDL3的方法和试剂，而不需要费力的操作。 用于定量高密度脂蛋白3中的胆固醇的方法包括使测试样品与一种或多种与高密度脂蛋白3特异性反应的表面活性剂反应并定量胆固醇。 当使用一种表面活性剂时，表面活性剂选自HLB为12.5〜15的聚氧乙烯多环苯基醚。当使用两种或更多种表面活性剂时，至少一种表面活性剂是选自下组中的至少一种： 由聚氧乙烯多环苯基醚组成，并且将两种或更多种表面活性剂组合以提供12.5至15的组合表面活性剂的总HLB。

    METHOD FOR MEASURING CHOLESTEROL IN ApoE-CONTAINING HDL
    23.
    发明公开
    METHOD FOR MEASURING CHOLESTEROL IN ApoE-CONTAINING HDL 有权
    EEREM APOE-HALTIGEN HDL中的VERFAHREN ZUR CHOLESTERINMESSUNG

    公开(公告)号：EP2495332A4

    公开(公告)日：2013-06-05

    申请号：EP10826682

    申请日：2010-10-26

    申请人： DENKA SEIKEN KK ,  UNIV HOKKAIDO NAT UNIV CORP

    发明人： ITOH YASUKI ,  CHIBA HITOSHI

    IPC分类号： C12Q1/60 ,  C12Q1/26 ,  C12Q1/44

    CPC分类号： C12Q1/60

    摘要： This invention provides a method for separately or simultaneously quantifying cholesterol in a total amount of HDL-C, cholesterol in an HDL subfraction of apoE-containing HDL-C, and cholesterol in an HDL subfraction of apoE-deficient HDL-C. The method comprises enzymatically and separately quantifying cholesterol in apoE-containing HDL and cholesterol in apoE-deficient HDL by adding a surfactant selected from the group consisting of a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 0.7 to less than 1.3, a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of less than 0.7, and a surfactant with an apoE-containing HDL response rate/apoE-deficient HDL response rate ratio of 1.3 or more to a test sample, allowing cholesterol esterase and cholesterol oxidase to react therewith, and quantifying the hydrogen peroxide generated.

    摘要翻译： 本发明提供了一种在apoE缺陷型HDL-C的HDL亚型分级中，分别或同时测定HDL-C，HDL-C，HDL-C，HDL-C中的胆固醇和胆固醇的胆固醇的方法。 该方法包括通过添加选自具有含apoE的HDL应答率/ apoE缺陷HDL应答率0.7的表面活性剂的表面活性剂来酶促分别定量含有apoE的HDL和胆固醇中的胆固醇的apoE缺陷型HDL中的胆固醇 小于1.3，具有apoE含量的HDL应答率/ apoE缺陷HDL响应率小于0.7的表面活性剂和具有apoE的HDL应答率/ apoE缺陷HDL应答率比率为1.3的表面活性剂 更多的是测试样品，允许胆固醇酯酶和胆固醇氧化酶与其反应，并量化产生的过氧化氢。

    METHOD FOR DETECTION OF FAMILIAL COMBINED HYPERLIPIDEMIA
    28.
    发明公开
    METHOD FOR DETECTION OF FAMILIAL COMBINED HYPERLIPIDEMIA 有权
    VERFAHREN ZUM NACHWEISFAMILIÄRERKOMBINIERTERHYPERLIPIDÄMIE

    公开(公告)号：EP2015077A4

    公开(公告)日：2009-08-12

    申请号：EP07742726

    申请日：2007-05-01

    申请人： DENKA SEIKEN KK

    发明人： ITOH YASUKI

    IPC分类号： G01N33/92

    CPC分类号： G01N33/92 ,  G01N2800/044 ,  G01N2800/323 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/25375

    摘要： The object is to provide an excellent method for detection/diagnosis of familial combined hyperlipemia. Disclosed is a method for detection of familial combined hyperlipemia, which comprises determining the small, dense LDL cholesterol level in a sample collected from a subject.

    摘要翻译： 目的是提供检测/诊断家族性高脂血症的极好方法。 公开了一种检测家族性合并高脂血症的方法，该方法包括确定从受试者收集的样品中的小而密LDL胆固醇水平。

    CONVENIENT DETECTION METHOD, DETECTION APPARATUS AND DETECTION KIT
    30.
    发明公开
    CONVENIENT DETECTION METHOD, DETECTION APPARATUS AND DETECTION KIT 有权
    TRUE的检测方法，检测装置和检测

    公开(公告)号：EP1712913A4

    公开(公告)日：2008-09-24

    申请号：EP05704193

    申请日：2005-01-27

    申请人： DENKA SEIKEN KK

    发明人： TAKIZAWA KAZUYUKI ,  SHIDA RYO

    IPC分类号： G01N33/543 ,  G01N21/78 ,  G01N33/53 ,  G01N33/58

    CPC分类号： G01N33/58 ,  G01N33/54366 ,  Y10S435/81 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S436/81 ,  Y10S436/818

    摘要： This invention provides a simple detection method with excellent sensitivity and specificity, which allows prompt detection of an analyte by allowing a labeled reagent to effectively react with the analyte in the process of treating an analyte-containing specimen, such as during removal of impurities, a detection apparatus, and a detection kit. This method for detecting an analyte in specimens comprises steps of: bringing a labeled reagent containing a ligand that specifically binds to the analyte into contact with a specimen; and supplying the mixture of the specimen and the labeled reagent to a solid-phase support onto which a capture reagent that specifically binds to the analyte has been immobilized, wherein the step of bringing the specimen into contact with the labeled reagent is carried out at a site that is not on the solid-phase support and that is detached from the solid-phase support. The invention also provides a detection apparatus comprising a solid-phase support onto which a capture reagent that specifically binds to the analyte in the specimen is immobilized and a kit for detecting an analyte in a specimen and a device for supplying a specimen comprising a labeled reagent containing a ligand that specifically binds to the analyte.