-
公开(公告)号：EP1616033A2
公开(公告)日：2006-01-18
申请号：EP04758226.7
申请日：2004-03-19
发明人： BORNS, Michael
CPC分类号： C12N15/102 , C12N9/1252 , C12Q1/6844 , C12Q1/686 , C12Q2527/119 , C12Q2522/101 , C12Q2521/101
摘要： The present invention discloses methods of using DNA polymerase fusions at high pH in PCR, DNA sequencing and mutagenesis protocols.
-
公开(公告)号：EP1578951A2
公开(公告)日：2005-09-28
申请号：EP03809647.5
申请日：2003-10-24
IPC分类号： C12N1/00
CPC分类号： C07H21/04 , C12N9/1252 , C12Q1/6869 , C12Q2535/101 , C12Q2521/101
摘要： The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3'-5' exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
摘要翻译： 本发明涉及具有降低的碱基模拟物检测活性的古细菌DNA聚合酶突变体的产生和表征。 本发明进一步提供了具有降低的碱性类似物检测活性的古细菌DNA聚合酶突变体，其含有调节其它DNA聚合酶活性的额外突变，包括DNA聚合或3'-5'外切核酸酶活性。 本发明还公开了具有降低的碱基类似物检测活性的DNA聚合酶的方法和应用。
-
公开(公告)号：EP1438430A4
公开(公告)日：2005-05-25
申请号：EP02763814
申请日：2002-10-02
发明人： TAYLOR ROGER H , ORR ALAN R
IPC分类号： G01N21/78 , C12N15/00 , C12P19/34 , C12Q1/68 , G01N33/48 , G01N33/50 , G06F19/00 , C07H19/00 , C07H21/02 , C07H21/04
CPC分类号： G01N21/6428 , C12Q1/6851 , C12Q2537/165
摘要： The invention relates to baseline subtraction algorithms developed to reduce tube-to-tube and cycle-to-cycle variabilities during real time PCR amplification. Particularly, the invention relates to algorithms for determining the threshold cycle for the first reliable detection of the amplified nucleic acid product. The invention also relates to computer programs comprising the algorithms and methods and/systems implementing the algorithms.
-
公开(公告)号：EP1504119A4
公开(公告)日：2005-02-09
申请号：EP01918206
申请日：2001-02-22
发明人： JI HENRY , GREENER ALAN L , SORGE JOSEPH A , BAUER JOHN , GIBBS RICHARD , CARSTENS CARSTEN-PETER
IPC分类号： C12N15/09 , C12N15/10 , C12N15/66 , C12Q1/68 , C07H21/02 , C07H21/04 , C12N9/00 , C12N15/64 , C12N15/74 , C12P19/34
摘要： The invention provides methods of covalently joining nucleic acid molecules and methods of molecular cloning. The methods provide either sequential or simultaneous ligation of flanking or vector nucleic acid molecules to nucleic acid insert molecules by topoisomerase and DNA ligase. The methods provide for directional and non-directional covalent joining and cloning of nucleic acid molecules.
-
公开(公告)号：EP1412491A4
公开(公告)日：2005-02-02
申请号：EP02803260
申请日：2002-06-27
发明人： SORGE JOSEPH A , HANSEN CONNI JO , HOGREFE HOLLY
IPC分类号： G01N33/53 , C12N9/12 , C12N15/09 , C12Q1/25 , C12Q1/48 , C12Q1/68 , G01N33/566 , G01N33/58 , C12P19/34
CPC分类号： B82Y5/00 , B82Y10/00 , C12N9/1252 , C12N9/1276
摘要： The invention features a novel isolated Family B DNA polymerase, a Thermococcus polymerase JDF-3, and mutant recombinant forms thereof. Mutant polymerases of the invention are deficient in 3’ to 5’ exonuclease activity and/or exhibit reduced discrimination against non-conventional nucleotides relative to the wild-type form of the polymerase.
-
公开(公告)号：EP1463808A2
公开(公告)日：2004-10-06
申请号：EP02795911.3
申请日：2002-12-17
CPC分类号： C12N9/1252
摘要： The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme; where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3'-5' exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
-
27.发明公开METHODS AND COMPOSITIONS FOR THE DETECTION OF A NUCLEIC ACID BY A CLEAVAGE REACTION 审中-公开方法和组合物核酸BY分解反应的检测
公开(公告)号：EP2094867A4
公开(公告)日：2010-08-04
申请号：EP07865723
申请日：2007-12-15
发明人： SORGE JOSEPH A , HAPPE SCOTT , FIRMIN ANDREW
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6823 , C12Q1/6825 , C12Q1/6827 , C12Q1/6844 , C12Q2565/1015 , C12Q2561/109 , C12Q2537/149 , C12Q2561/113 , C12Q2525/186 , C12Q2525/161 , C12Q2521/301 , C12Q2531/119 , C12Q2531/101
-
28.发明公开SYSTEM AND METHOD FOR FLUORESCENCE EXCITATION AND DETECTION HAVING DISTINCT OPTICAL PATHS 审中-公开系统和方法用于荧光激发和检测具有不同的光路
公开(公告)号：EP1893975A4
公开(公告)日：2010-08-04
申请号：EP06784804
申请日：2006-06-12
发明人： REID TAYLOR A , TAYLOR ROGER H , ZAJAC KENNETH J
IPC分类号： G01N21/01
CPC分类号： G01J3/10 , G01J3/4406 , G01J2003/1213 , G01N21/645 , G01N21/6452 , G01N21/6486 , G01N2021/6484 , G01N2201/0446 , G01N2201/0612 , G01N2201/062
-
公开(公告)号：EP1910534A4
公开(公告)日：2010-03-10
申请号：EP06787626
申请日：2006-07-17
发明人： BORNS MICHAEL
CPC分类号： C12N9/1252 , C07K2319/80
-
公开(公告)号：EP2110432A3
公开(公告)日：2009-11-11
申请号：EP09100097.6
申请日：2002-12-17
发明人： Hogrefe, Hooly , Borns, Michael , Sorge, Joseph A.
CPC分类号： C12N9/1252
摘要： The subject invention relates to compositions comprising an enzyme mixture which comprises a first enzyme and a second enzyme; where the first enzyme comprises a DNA polymerization activity and the second enzyme comprises an 3'-5' exonuclease activity and a reduced DNA polymerization activity. The invention also relates to the above compositions in kit format and methods for high fidelity DNA synthesis using the subject compositions of the invention.
摘要翻译： 本发明涉及包含含有第一种酶和第二种酶的酶混合物的组合物; 其中第一种酶包含DNA聚合活性并且第二种酶包含3'-5'外切核酸酶活性和降低的DNA聚合活性。 本发明还涉及用于使用本发明的主题组合物进行高保真度DNA合成的试剂盒形式和方法的上述组合物。
-
-
-
-
-
-
-
-
-
搜索结果
81 条记录 (耗时 0.025 秒)
