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124 条记录 (耗时 0.026 秒)

    FLOW CYTOMETER
    21.
    发明授权
    FLOW CYTOMETER 失效
    流式细胞仪

    公开(公告)号：EP0752133B1

    公开(公告)日：2000-06-28

    申请号：EP94908640.9

    申请日：1994-01-25

    申请人： COULTER CORPORATION

    发明人： AUER, Robert, E. ,  WEBER, Bruce, M. ,  STARLING, John, D. ,  WOOD, James, C., S.

    IPC分类号： G01N15/14 ,  G06F17/40 ,  G06F19/00

    CPC分类号： G01N15/14 ,  G01J2001/4406 ,  G01N15/1456

    摘要： A flow cytometer (10) includes a plurality of detectors (28, 30, 32, 34, 36, 38) for providing voltage pulse signals over a four decade range as particles pass through an illuminated detection station (20). Each voltage pulse signal is processed and provided to a first sample and hold (84, 138) for storage. Thereafter, the value in the first sample and hold is amplified by one or thirty-two, depending upon its magnitude, and stored in a second sample and hold (84, 146) for subsequent provision to a sixteen bit analog to digital convertor (ADC) (66), one signal at a time. The first sample and hold is then free to store a new value while the old value is awaiting provision to the ADC. The ADC converts the signal received from the second sample and hold to a fifteen bit digital signal and uses the sixteenth bit to manifest whether the signal stored in the second sample and hold was amplified by thirty-two.

    METHOD FOR SCREENING CELLS OR FORMED BODIES WITH POPULATIONS EXPRESSING SELECTED CHARACTERISTICS
    22.
    发明授权
    METHOD FOR SCREENING CELLS OR FORMED BODIES WITH POPULATIONS EXPRESSING SELECTED CHARACTERISTICS 失效
    与选定的条件选择种群某些细胞或细​​胞结构BASIS方法

    公开(公告)号：EP0472522B1

    公开(公告)日：1997-05-28

    申请号：EP90901352.6

    申请日：1989-12-13

    申请人： COULTER CORPORATION

    发明人： KORTRIGHT, Kenneth, H. ,  COULTER, Wallace, H. ,  RODRIGUEZ, Carlos ,  RUSSELL, Thomas ,  PAUL, Ronald ,  HAJEK, Constance Mary ,  HUDSON, James Carey

    IPC分类号： G01N15/12

    CPC分类号： G01N15/12 ,  G01N33/56972 ,  G01N2015/008 ,  G01N2015/1062 ,  G01N2015/1081

    摘要： A method and apparatus (320, 340, 360) for automatically and rapidly retrieving, counting and/or analyzing at least one selected population of cells or formed bodies, such as a white blood cell population and at least one subset thereof of a whole blood sample (322, 342) or portion thereof. A volume of a biological medium containing the white blood cells is prepared and at least one reactant (326, 346) specific or preferential at least to some selected biological cells is introduced thereto and rapidly mixed (384) for a short period of time. The opacity and/or volume parameter of the cells can be modified and the mixture is then counted and analyzed (332, 352, 416, 422) in one or more steps to obtain the desired white blood cell population and subset analysis. The biological sample can be a whole blood sample and the reactant can include or be a lyse or a monoclonal antibody bound to microspheres, which will bind to specific ones of the cells or a combination of lyse and microspheres with antibody bound thereto. The microspheres can be magnetic and the bound cells can be magnetically removed for retrieving and analyzing the remaining blood cell population.

    COVALENT IMMOBILIZED ANTIBODIES ON POLYMERIC BEADS
    24.
    发明公开
    COVALENT IMMOBILIZED ANTIBODIES ON POLYMERIC BEADS 失效
    共价固定抗体ON聚合物微珠

    公开(公告)号：EP0752097A1

    公开(公告)日：1997-01-08

    申请号：EP95913509.0

    申请日：1995-03-02

    申请人： COULTER CORPORATION

    发明人： SIIMAN, Olavi ,  BURSHTEYN, Alexander

    IPC分类号： G01N33 ,  C07K16 ,  G01N15 ,  G01N21

    CPC分类号： G01N33/56972 ,  G01N33/54313 ,  G01N33/54353

    摘要： The invention relates to polymeric colloidal particles having a short, low molecular weight diamine spacer or polymeric, crosslinked aminodextran coating that is functionalized to bind a pendent protein such as antibody, to methods of making such particles, to the use of such particles in biological assays, to the effects of thermal and freeze-drying treatment of such particles on their stability and structure as dectected from their interactions with leukocytes in biological assays with whole blood on the nonspecific interactions between leukocytes and aminodextran-coated beads.

    SYSTEM FOR ISOLATING AND IDENTIFYING LEUKOCYTES
    25.
    发明授权
    SYSTEM FOR ISOLATING AND IDENTIFYING LEUKOCYTES 失效
    系统分离和白细胞鉴定

    公开(公告)号：EP0305491B1

    公开(公告)日：1996-02-07

    申请号：EP88903078.9

    申请日：1988-03-11

    申请人： COULTER CORPORATION

    发明人： LEDIS, Stephen, L. ,  CREWS, Harold, R. ,  FISCHER, Timothy, J. ,  SENA, Ted

    IPC分类号： G01N1/28 ,  G01N33/50

    CPC分类号： G01N33/5094 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331

    摘要： A method and reagent system for the rapid isolation, identification, and/or analysis of leukocytes from a whole blood sample. The reagent system includes a lytic reagent solution comprising an aqueous solution of a water soluble compound which at least partially dissociates in aqueous solution to release a proton and a counterion and which, upon contact with a blood sample, acidifies the sample to a pH in the range of from about 2.6 to 4.0. The reagent system also includes a quenching reagent solution comprising an aqueous alkaline salt solution which effectively and rapidly arrests the chemical action of the lytic reagent solution on the sample and restores the native physiological environment of the sample. The method comprises using the reagent system to selectively lyse the red blood cells in a blood sample and modify the leukocytes to enable their differentiation into five sub-populations.

    METHOD OF PREFERENTIAL LABELLING OF PHYCOBILIPROTEIN WITH AMINE-REACTIVE DYE FOR USE IN A MULTIPLE COLOR ASSAY
    26.
    发明公开
    METHOD OF PREFERENTIAL LABELLING OF PHYCOBILIPROTEIN WITH AMINE-REACTIVE DYE FOR USE IN A MULTIPLE COLOR ASSAY 失效
    具体MARKING藻胆蛋白方法意味着FOR USE IN A MULTI-COLOR TEST胺反应性染料。

    公开(公告)号：EP0673392A1

    公开(公告)日：1995-09-27

    申请号：EP93921202.0

    申请日：1993-08-27

    申请人： COULTER CORPORATION

    发明人： GUPTA, Ravinder K.

    IPC分类号： G01N33 ,  C07K1 ,  C07K14 ,  G01N30

    CPC分类号： G01N33/542 ,  G01N33/72

    摘要： A method for preparing phycobiliprotein/amine-reactive dye conjugates is disclosed in which the conjugates so prepared overcome the energy transfer/fluorescent quenching dilemma encountered in the use of prior art conjugates. A phycobiliprotein, for example, phycoerythrin or allophycocyanin, is conjugated with an amine-reactive dye, for example, Texas Red or carboxyfluorescein succinimidyl ester, in the presence of a selective salt which causes a hydrophobic intramolecular rearrangement of the phycobiliprotein thereby exposing more hydrophobic sites for binding to the amine-reactive dye. The conjugates prepared according to the invention are useful in multiple color fluorescence assays without requiring the use of multiple exciting sources.