公开(公告)号：EP2647714A3

公开(公告)日：2013-10-23

申请号：EP13162003.1

申请日：2013-04-02

申请人： Kikkoman Corporation

发明人： Takahashi, Tadashi

IPC分类号： C12N15/80

CPC分类号： C12N15/80 ,  A23L27/50

摘要： The purpose of the present invention is to provide a technique to duplicate and translocate any large region in a chromosome of a fungus belonging to Aspergillus across a wide range, so that it will possible to stably and systematically acquire an Aspergillus strain having a novel trait, which was un-acquirable by conventional techniques.
The present invention relates to a transformant of the fungus belonging to Aspergillus wherein a transformation marker gene with deficiency of a terminal part at the 5' or 3' end of its coding region is integrated into an outside of a target region in a chromosome of the fungus subject to duplicated translocation, and a transformation marker gene with deficiency of a terminal part at the 3' or 5' end of its coding region is integrated into an outside of a region in another chromosome of the fungus to be replaced with the target region.

公开(公告)号：EP2647705A1

公开(公告)日：2013-10-09

申请号：EP11845337.2

申请日：2011-11-30

申请人： Kikkoman Corporation

发明人： TAJIMA, Ryoko ,  ICHIYANAGI, Atsushi ,  HIROKAWA, Kozo ,  YUZUKI, Masanobu

IPC分类号： C12N15/09 ,  C12N1/19 ,  C12N9/04 ,  C12P21/02

CPC分类号： C12N9/0006 ,  C12N1/16 ,  C12N15/81 ,  C12Y101/9901

摘要： The present invention provides heat-resistant flavin-bound glucose dehydrogenases having high substrate specificity for D-glucose, an efficient production method of the same, and a transformant used for the same.
A flavin-bound glucose dehydrogenase gene encoding a flavin-bound glucose dehydrogenase derived from Mucor is introduced into yeast, Zygosaccharomyces, to obtain a transformant. Subsequently, the yeast transformant is cultured to obtain a flavin-bound glucose dehydrogenase from the culture.
A heat-resistant flavin-bound glucose dehydrogenase that is less susceptible to the effects of dissolved oxygen and allows accurate measurement of glucose even in the presence of sugar compounds other than glucose in a sample can be efficiently produced.

摘要翻译： 本发明提供了对D-葡萄糖具有高底物特异性的耐热黄素结合葡萄糖脱氢酶，其有效的制备方法和用于其的转化体。 将编码来自毛果的黄素结合的葡萄糖脱氢酶的黄素结合的葡萄糖脱氢酶基因导入酵母（Zygosaccharomyces），得到转化体。 随后，培养酵母转化体以从培养物中获得与黄素结合的葡萄糖脱氢酶。 可以有效地制备耐溶解黄素结合的葡萄糖脱氢酶，其对溶解氧的影响不太敏感，并且即使在样品中除了葡萄糖以外的糖化合物的存在下也能够精确测量葡萄糖。

公开(公告)号：EP2602318A1

公开(公告)日：2013-06-12

申请号：EP11814719.8

申请日：2011-08-04

申请人： KIKKOMAN CORPORATION

发明人： ICHIYANAGI Atsushi ,  HIROKAWA Kozo ,  TANABE Yasuko ,  MASAKARI Yosuke

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/04 ,  C12Q1/26

CPC分类号： C12N9/0032 ,  C12N1/00 ,  C12N15/63 ,  C12Q1/26 ,  G01N33/723 ,  G01N2333/902

摘要： This invention provides an amadoriase having high substrate specificity to fructosyl valyl histidine. Such amadoriase comprises substitution of one or more amino acid residues at positions corresponding to amino acids selected from the group consisting of position 98, position 259, position 154, position 125, position 261, position 263, position 106, position 103, position 355, position 96, position 66, position 67, position 70, position 100, position 110, position 113, position 114, and position 156 in the amadoriase derived from the genus Coniochaeta. This invention enables accurate measurement of α-fructosyl valyl histidine derived from the β-chain amino terminus in glycated hemoglobin in the presence of ε-fructosyl lysine.

摘要翻译： 本发明提供对果糖基缬氨酰组氨酸具有高底物特异性的阿马道里酶。 这种阿马道里酶包括在对应于选自第98位，259位，154位，125位，261位，263位，106位，103位，355位氨基酸的位置上的一个或多个氨基酸残基取代。 位于第59位，位置66，位置67，位置70，位置100，位置110，位置113，位置114和位置156在源自Coniochaeta的阿马多利亚酶。 本发明能够在存在μ-果糖基赖氨酸的情况下，精确测定衍生自糖基化血红蛋白的2-链氨基末端的β-果糖基缬氨酰基组氨酸。

公开(公告)号：EP2499923A1

公开(公告)日：2012-09-19

申请号：EP10828404.3

申请日：2010-11-09

申请人： Kikkoman Corporation

发明人： TSUCHIYA Katsunori ,  UCHIDA Riichiro ,  TODA Yasuka

IPC分类号： A23L1/238

CPC分类号： A23L27/50 ,  A23L27/40

摘要： By allowing a liquid seasoning to comprise from 0.2% to 4% (w/v) in concentration of ammonium ion and from 0.5% to 7% (w/v) in containing amount of common salt and adjusting pH to from 3.2 to 5.4, it became possible to provide a liquid seasoning which is excellent in salty taste and tastiness regardless of its low common salt content.

摘要翻译： 通过允许液体调味料含有0.2％至4％（w / v）的铵离子浓度和0.5％至7％（w / v）的含盐量，并将pH调节至3.2至5.4， 无论其常见的盐含量低，都可以提供优良的咸味和口味的液体调味料。

公开(公告)号：EP2407551A2

公开(公告)日：2012-01-18

申请号：EP11180939.8

申请日：2008-11-19

申请人： Kikkoman Corporation

发明人： Suzuki, Shigeya ,  Kodama, Yukako ,  Kurosawa, Keiko ,  Kobatake, Eiry ,  Mie, Masayasu

IPC分类号： C12Q1/66 ,  C12Q1/68 ,  C12N9/02 ,  C12N15/09

CPC分类号： C12Q1/66

摘要： The present invention provides a firefly luciferase for inexpensive, highly accurate and highly sensitive nucleic acid analysis that uses dATP instead of an expensive reagent having low reactivity to DNA polymerase in the manner of dATPαS, a method of analyzing nucleic acid that uses that luciferase, and a kit for analyzing nucleic acid thereof.
The present invention relates to a composition for analyzing nucleic acid that contains luciferase for which reactivity to dATP is equal to or less than 1/400 reactivity to ATP, a method of analyzing nucleic acid that comprises the use of that composition, and a kit for analyzing nucleic acid comprising that composition.

摘要翻译： 本发明提供了一种用于廉价，高精确度和高灵敏度的核酸分析的萤火虫萤光素酶，其使用dATP代替以dATPαS的方式对DNA聚合酶具有低反应性的昂贵试剂，分析使用该萤光素酶的核酸的方法，以及 用于分析其核酸的试剂盒。 本发明涉及用于分析核酸的组合物，其包含对dATP的反应性等于或小于1/400对ATP的反应性的荧光素酶，分析包括使用该组合物的核酸的方法和用于 分析包含该组合物的核酸。

公开(公告)号：EP2348108A1

公开(公告)日：2011-07-27

申请号：EP10783216.4

申请日：2010-04-19

申请人： Kikkoman Corporation

发明人： TAJIMA, Ryoko ,  ICHIYANAGI, Atsushi ,  ICHIKAWA, Keiichi ,  YOSHIMURA, Taro

IPC分类号： C12N15/09 ,  C12N9/04

CPC分类号： C12N9/0006

摘要： There are provided flavin-binding glucose dehydrogenases with high substrate specificity for D-glucose.
Novel flavin-binding glucose dehydrogenases which are derived from microorganisms belonging to the genus Mucor. They have low reactivity for maltose, D-galactose and D-xylose compared to their reactivity for D-glucose, and are therefore relatively unaffected by these saccharide compounds.
They are also relatively unaffected by dissolved oxygen, and allow accurate measurement of glucose amounts even in the presence of saccharide compounds other than glucose in samples.

摘要翻译： 提供了对D-葡萄糖具有高底物特异性的黄素结合葡萄糖脱氢酶。 衍生自属于毛霉属的微生物的新型黄素结合性葡萄糖脱氢酶。 它们与D-葡萄糖的反应性相比对麦芽糖，D-半乳糖和D-木糖具有低反应性，因此相对不受这些糖类化合物的影响。 它们也相对不受溶解氧的影响，并且即使在样品中除了葡萄糖以外的糖类化合物的存在下也允许精确测量葡萄糖量。

公开(公告)号：EP2241194A1

公开(公告)日：2010-10-20

申请号：EP07860174.7

申请日：2007-12-26

申请人： Kikkoman Corporation

发明人： TOBE, Katsutoshi ,  TANNO, Takeki ,  EGAWA, Isao

IPC分类号： A23L1/238 ,  A23L1/10

CPC分类号： A23L27/50 ,  A23L7/196 ,  A23L7/1975

摘要： It is an object of the present invention to provide soy sauce characterized by a low turbidity in the raw soy sauce state, reduced formation of pasteurized sediment in the soy sauce pasteurization step, and a light color. The present invention relates to soy sauce produced by koji production, fermentation and aging with the use of a protein material and a starch material as starting materials, wherein the starch material is gelatinized brown rice having a moisture content of 1% (w/w) or less, a degree of gelatinization of 40% or more, and no burnt odor.

摘要翻译： 本发明目的在于提供由低浊度在生酱油状态为特征的酱油，减少了酱油巴氏杀菌步骤加热沉渣的形成，以及光颜色的目的。 本发明涉及通过曲生产，发酵和与使用蛋白质原料和淀粉质原料用作原料，worin淀粉材料的老化产生酱油凝胶化具有1％的水分含量糙米（重量/重量） 以下，化度在40％以上，且没有焦臭的α化。

公开(公告)号：EP1988155B1

公开(公告)日：2010-07-28

申请号：EP07714723.9

申请日：2007-02-21

申请人： KIKKOMAN CORPORATION

发明人： HANYA, Yoshiki ,  SATO, Itsuo ,  SHIMOJO, Ryo

IPC分类号： C12N1/20 ,  C12P13/00 ,  C12R1/225

CPC分类号： C12R1/225 ,  C12N1/20 ,  C12P13/005

公开(公告)号：EP2169057A1

公开(公告)日：2010-03-31

申请号：EP08777818.9

申请日：2008-07-03

申请人： Kikkoman Corporation

发明人： The designation of the inventor has not yet been filed

IPC分类号： C12N15/09 ,  A61K31/713 ,  A61K35/74 ,  A61P37/02 ,  C12P19/34

CPC分类号： A61K31/713 ,  A61K35/744 ,  A61K35/747 ,  C12N15/117 ,  C12N2310/17 ,  C12N2330/10

摘要： An immunoregulator that is safe and efficiently incorporated into cells along with a production process thereof are provided. Double-stranded RNA derived from lactic acid bacteria, an immunoregulator having for an active ingredient thereof double-stranded RNA derived from lactic acid bacteria, and a process for producing double-stranded RNA derived from lactic acid bacteria are provided. Bacteria cells of a strains of lactic acid bacteria such as genus Tetragenococcus, genus Pediococcus, genus Lactobacillus, genus Streptococcus or genus Leuconostoc are able to produce double-stranded RNA having immunoregulatory action therein.

摘要翻译： 提供了一种安全有效地并入细胞中的免疫调节剂及其制备方法。 提供了衍生自乳酸菌的双链RNA，具有活性成分的来自乳酸菌的双链RNA的免疫调节剂，以及由乳酸菌制备双链RNA的方法。 乳酸菌菌株如四源球菌属，球菌属，乳杆菌属，链球菌属或明串珠菌属的细菌细胞能够产生其中具有免疫调节作用的双链RNA。

公开(公告)号：EP1731600B1

公开(公告)日：2009-03-18

申请号：EP06011850.2

申请日：2006-06-08

申请人： KIKKOMAN CORPORATION

发明人： Sakaue, Ryoichi ,  Kajiyama, Naoki

IPC分类号： C12N9/04

CPC分类号： C12N9/0006