公开(公告)号：EP0696163B1

公开(公告)日：1998-08-05

申请号：EP94915180.7

申请日：1994-04-22

申请人： RHONE-POULENC AGRO

发明人： DUPUIS, Jean-Marc ,  MOLLE, Francis

IPC分类号： A01H4/00

CPC分类号： A01H4/00

摘要： Coated meristematic tissue comprising a dry or dryable meristematic tissue (1) and solid support (3) in contact with said tissue and partially covering it, in such a manner that the tissue remains in contact with a volume of air (2). The support is capable of absorbing a nutrient medium and germinating. The invention is suitable for seeding or culturing purposes.

公开(公告)号：EP0485506B1

公开(公告)日：1997-11-12

申请号：EP90912722.7

申请日：1990-08-08

申请人： DeKalb Genetics Corporation

发明人： ADAMS, Thomas, R. ,  ADAMS, Whitney, R., Jr. ,  CHAMBERS, Sheryl, A. ,  DAINES, Richard, J. ,  GORDON-KAMM, William, J. ,  KAUSCH, Albert, P. ,  KRUEGER, Roger, W. ,  LEMAUX, Peggy, G. ,  MACKEY, Catherine, J. ,  MANGANO, Mary, L. ,  O'BRIEN, James, V. ,  RICE, Thomas, B. ,  SPENCER, T., Michael ,  START, William, G. ,  WILLETTS, Nancy

IPC分类号： C12N15/82 ,  A01H4/00 ,  A01H5/00 ,  C12N5/00

CPC分类号： C12N15/87 ,  A01H4/00 ,  A01H4/001 ,  C07K14/415 ,  C12N5/0025 ,  C12N5/04 ,  C12N15/8207

摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.

公开(公告)号：EP0720424A1

公开(公告)日：1996-07-10

申请号：EP94928424.0

申请日：1994-09-21

申请人： L.V.M.H. RECHERCHE

发明人： MAES, Olivier ,  JOUENNE, Thierry ,  GUERN, Jean ,  COUTOS-THEVENOT, Pierre

IPC分类号： A01H4 ,  C12N5

CPC分类号： A01H4/00 ,  C12N5/0025

摘要： A method for encouraging plant cell differentiation in culture conditions, particularly for tissular differentiation, embryogenesis, and/or organogenesis, is characterized in that an adequate concentration of at least one proteolytic enzyme is introduced into the culture medium for cell differentiation.

公开(公告)号：EP0716708A1

公开(公告)日：1996-06-19

申请号：EP94928311.0

申请日：1994-09-02

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： TÖPFER, Reinhard ,  MARTINI, Norbert ,  SCHELL, Jozef

IPC分类号： A01H4 ,  C12N9 ,  C12N15

CPC分类号： C12N9/16 ,  A01H4/00 ,  C12N15/8247

摘要： DNA sequences are disclosed that code for a middle chain-specific acyl-[ACP]-thioesterase, as well as alleles and derivatives of said DNA sequence. This DNA may be used to transform plants capable of forming middle chain-specific acyl-[ACP]-thioesterases.

公开(公告)号：EP0696163A1

公开(公告)日：1996-02-14

申请号：EP94915180.0

申请日：1994-04-22

申请人： RHONE-POULENC AGROCHIMIE

发明人： DUPUIS, Jean-Marc ,  MOLLE, Francis

IPC分类号： A01C1 ,  A01H4 ,  C12N5

CPC分类号： A01H4/00

摘要： Coated meristematic tissue comprising a dry or dryable meristematic tissue (1) and solid support (3) in contact with said tissue and partially covering it, in such a manner that the tissue remains in contact with a volume of air (2). The support is capable of absorbing a nutrient medium and germinating. The invention is suitable for seeding or culturing purposes.

公开(公告)号：EP0688160A1

公开(公告)日：1995-12-27

申请号：EP94908900.0

申请日：1994-03-10

申请人： NATIONAL RESEARCH COUNCIL OF CANADA

发明人： NEHRA, Narender, S. ,  KARTHA, Kutty, K. ,  CHIBBAR, Ravindra, N.

IPC分类号： A01H4

CPC分类号： A01H4/00

摘要： Whole scutella are isolated from immature zygotic embryos of cereal plants and cultured, in the absence of the zygotic embryo axis, to produce somatic embryos, which in turn are converted into plantlets. The scutellar cells optionally are transformed with foreign DNA so that at least some of the resulting plantlets are transgenic. The regeneration is much more efficient and rapid than with conventional methods.

公开(公告)号：EP0633719A4

公开(公告)日：1995-12-20

申请号：EP93908652

申请日：1993-03-19

申请人： UNION CAMP CORP ,  UNION CAMP CORP

发明人： WANN STEVEN R ,  GOLDNER WILLIAM R

IPC分类号： A01H4/00 ,  A01H7/00 ,  C12N5/04 ,  C12N15/09 ,  A01H1/00

CPC分类号： A01H7/00 ,  A01H4/00

摘要： The present invention provides methods of inducing somatic embryogenesis in tissue cultures derived from varieties of Taxus. In addition, the present invention provides methods of obtaining taxol, or precursors thereof, using in vitro propagated somatic embryo tissues.

公开(公告)号：EP0433394B1

公开(公告)日：1995-11-08

申请号：EP89911155.3

申请日：1989-09-05

申请人： YAMASHITA, Thomas T.

发明人： YAMASHITA, Thomas T.

IPC分类号： C05C9/00 ,  C05G5/00 ,  A01C1/06

CPC分类号： C05G3/0076 ,  A01C1/06 ,  A01G7/06 ,  A01H1/025 ,  A01H4/00 ,  C05F11/00 ,  C05G1/00 ,  C05G3/0064 ,  Y02P20/134 ,  Y02P60/12 ,  C05F11/08 ,  C05F11/10

摘要： Method of and composition for treating vegetation to improve its growth or yield of a crop. The composition comprises a carbon skeleton/energy component, such as for example molasses, a macronutrient component and a micronutrient component, preferably also a vitamin/cofactor component and an enhancement component. The composition is usually in the form of an aqueous solution. It may be applied as a foliar spray or in other ways; it is preferably applied in increments; and it makes use of the natural ability of vegetation to harvest solar energy and other sources of energy and carbon skeleton. Where applied to pollen, anthers (18) may be dried in revolving tubes (10), then crushed and separated from debris on a shake table (25).

公开(公告)号：EP0589357A3

公开(公告)日：1994-06-01

申请号：EP93114912.4

申请日：1993-09-16

申请人： MADAUS Aktiengesellschaft

发明人： Schieder, Otto, Prof. Dr. ,  Hetz, Erich ,  Liersch, Reinhard, Dr.

IPC分类号： A01H4/00

CPC分类号： A01H4/00 ,  A01H5/02 ,  A01H6/14

摘要： Die vorliegende Erfindung betrifft ein Verfahren zur Herstellung genetisch identischer Pflanzen der Gattung Silybum , das dadurch gekennzeichnet ist, daß man aus einer Donorpflanze definierten Genotyps Protoplasten herstellt, und anschließend aus den Protoplasten genetisch identische Pflanzen der Gattung Silybum regeneriert. Gegenstand der Erfindung sind außerdem die mit Hilfe dieses Verfahrens erhältlichen Protoplasten.

公开(公告)号：EP0561082A1

公开(公告)日：1993-09-22

申请号：EP92311126.4

申请日：1992-12-07

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： Buising, Charisse Marie ,  Tomes, Thomas ,  Schmidt, Janice

IPC分类号： C12N15/87 ,  C12N15/82 ,  C12N5/14 ,  C12N5/04 ,  A01H1/00 ,  A01H4/00

CPC分类号： A01H4/008 ,  A01H1/00 ,  A01H4/00 ,  C12N15/8207

摘要： The invention relates to improved transformation and regeneration of alfalfa, Medicago sativa .
A method is used to transform alfalfa by using particle acceleration. Optimum results with mature cotyledons occurs when bombarding after 24 to 120 hours of imbibing water. Regeneration and transformation of alfalfa is greatly improved by using immature cotyledons or embryos of immature cotyledons for transformation and regeneration Immature cotyledons include those up to about 25 days past pollination, and preferably include cotyledons excised at 10-15 days past pollination, most preferably including those excised at about 10 days past pollination. These cotyledons have a light green to translucent appearance. Plants resulting from bombardment of somatic embryos of immature cotyledons retain regeneration ability.

摘要翻译： 本发明涉及改进苜蓿，苜蓿的转化和再生。 一种使用粒子加速度来转换苜蓿的方法。 成熟子叶的最佳结果发生在24至120小时吸水后进行轰击。 通过使用未成熟的子叶或未成熟子叶的胚胎进行转化和再生，可以大大提高苜蓿的再生和转化。未成熟子叶包括从授粉到大约25天的子叶，优选包括在授粉后10-15天切除的子叶，最优选包括那些 在授粉约10天后切除。 这些子叶具有浅绿色至半透明的外观。 由未成熟子叶的体细胞胚发生的植物保留再生能力。