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公开(公告)号：EP2040983A4
公开(公告)日：2011-08-03
申请号：EP07809899
申请日：2007-06-26
发明人： HIRANO KIRK M , NURSE JAMES C , CARRILLO ALBERT L , LU MIKE Y , FAWCETT ADRIAN
IPC分类号： B65B7/28
CPC分类号： G01N35/028 , B01L3/50851 , B01L3/50853 , B01L3/563 , B01L7/52 , B01L2200/0605 , B01L2200/0642 , B01L2200/142 , B01L2300/041 , B01L2300/044 , B01L2300/046 , B01L2300/0654 , B01L2300/0829 , B01L2300/0864 , B01L2300/1822 , B01L2300/1827 , B01L2300/1872 , B01L2400/0406 , B01L2400/0487 , G01N2035/00287
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公开(公告)号：EP1513949A4
公开(公告)日：2006-02-15
申请号：EP03722089
申请日：2003-05-30
CPC分类号： B04B15/02 , B01L7/52 , B01L2200/147 , B01L2300/0803 , B01L2300/1844 , B01L2300/1872 , C12Q1/686 , C12Q2563/173
摘要： The invention relates to a method for the amplification of DNA and apparatus for such amplification. The method comprises steps typically found in amplification methods but utilises an optical procedure to detect denaturation of DNA or attainment of the desired denaturation temperature. The apparatus (1) comprises a temperature controllable chamber (2) including a rotor (3) for holding a plurality of reaction vessels (see 7 for example) for reaction mixtures including DNA, a drive means for the rotor, a heater (8) within the chamber for transiently supplying infrared energy to the reaction vessels, and an optical system (12-16) for determining denaturation of at least a reference DNA or for detecting attainment of a desired denaturation temperature in at least a reference reaction vessel.
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公开(公告)号：EP3077114A1
公开(公告)日：2016-10-12
申请号：EP14786400.3
申请日：2014-09-29
CPC分类号： B01L7/52 , B01L3/5027 , B01L2200/147 , B01L2300/0627 , B01L2300/0816 , B01L2300/1822 , B01L2300/1844 , B01L2300/1872 , B01L2300/1894 , C12Q1/686 , C12Q2565/629
摘要： A microfluidic chip includes one or more reaction chambers to hold fluids for chemical or biochemical reactions, such as PCR. A non-contact heat source heats the reaction chamber and the fluid, such that the heat source does not contact the reaction chamber or the fluid. The heat source can heat the reaction chamber and the fluid separately, where the reaction chamber and the fluid separately absorb heat radiation from the heat source. A temperature sensor acquires a temperature of the reaction chamber and/or the fluid. Control circuitry controls the heat source according to a cycling profile for the reaction in the fluid to cycle the heat source between heating and not heating the reaction chamber and the fluid based on the temperature acquired by the temperature sensor. Cooling can be provided passively or actively.
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公开(公告)号：EP2969214A1
公开(公告)日：2016-01-20
申请号：EP14712711.2
申请日：2014-03-13
申请人： BJS IP Limited
发明人： BURROUGHS, Nicholas
CPC分类号： C12Q1/686 , B01L3/50851 , B01L7/52 , B01L7/525 , B01L2300/0829 , B01L2300/12 , B01L2300/1811 , B01L2300/1822 , B01L2300/1866 , B01L2300/1872 , B01L2300/1894
摘要： Provided herein is a microplate for polymerase chain reaction (PCR), comprising a substrate formed of a material that is susceptible to heating PCR samples upon the application of an electromagnetic field and/or electromagnetic energy to said substrate. The substrate provides a PCR ramp rate of at least 5°C/second upon the application of an electromagnetic field and/or electromagnetic energy to said substrate.
摘要翻译： 本文提供了用于聚合酶链式反应（PCR）的微板，其包含由在对所述基板施加电磁场和/或电磁能时加热PCR样品敏感的材料形成的基板。 在向所述衬底施加电磁场和/或电磁能时，衬底提供至少5℃/秒的PCR斜坡速率。
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公开(公告)号：EP2942103A1
公开(公告)日：2015-11-11
申请号：EP14167075.2
申请日：2014-05-05
发明人： Seidel, Susanne , Braun, Dieter , Duhr, Stefan , Baaske, Philipp
CPC分类号： G01N21/6486 , B01L3/0268 , B01L3/5027 , B01L3/50851 , B01L2200/0642 , B01L2200/0673 , B01L2300/0663 , B01L2300/0819 , B01L2300/0829 , B01L2300/0838 , B01L2300/1872 , B01L2400/0451 , G01N15/0205 , G01N21/645 , G01N21/6456 , G01N2201/06113
摘要： The present invention relates generally to a system and a method for thermo-optical measurements in a droplet (23) of aqueous solution comprising particles of interest, the method comprising the following steps: providing the droplet of aqueous solution with a volume of less than 200nl, wherein the aqueous solution is a first liquid (21) and at least a part of the particles of interest are fluorescent particles; embedding the droplet (23) of aqueous solution at least partly in a second liquid (21); irradiating a laser light beam into the droplet to obtain a spatial temperature distribution in the droplet around the irradiated laser light beam; exciting fluorescently said fluorescent particles and detecting the fluorescence at least at one position or at around one position in the droplet or detecting the fluorescence distribution of said fluorescently excited particles, wherein said detection of fluorescence is performed at least once at a predetermined time after the start of the laser irradiation; and determining a characteristic of the particles of interest from the detected fluorescence intensity or fluorescence intensity distribution.
摘要翻译： 本发明一般涉及用于在包含感兴趣的颗粒的水溶液的液滴（23）中进行热光学测量的系统和方法，该方法包括以下步骤：提供具有小于200nl的体积的水溶液液滴 其中所述水溶液是第一液体（21）并且至少部分所述目标颗粒是荧光颗粒; 将水溶液液滴（23）至少部分地嵌入第二液体（21）中; 将激光束照射到液滴中以获得照射的激光束周围的液滴中的空间温度分布; 将所述荧光粒子荧光地激发并且至少在所述液滴中的一个位置或者在所述液滴的一个位置处检测所述荧光，或者检测所述荧光激发粒子的荧光分布，其中所述荧光检测在所述开始之后的预定时间执行至少一次 的激光照射; 并根据检测到的荧光强度或荧光强度分布确定感兴趣的颗粒的特征。
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公开(公告)号：EP2617806A1
公开(公告)日：2013-07-24
申请号：EP10857326.2
申请日：2010-11-15
发明人： CHUN, Hyang Sook , CHOI, Sung Wook , LEE, Na Ri , CHANG, Hyun Joo
CPC分类号： B01L7/52 , B01L7/5255 , B01L2300/0803 , B01L2300/0829 , B01L2300/168 , B01L2300/1844 , B01L2300/1861 , B01L2300/1872 , C12Q1/6844
摘要： Disclosed is a non-contact heating type gene amplification system. The non-contact heating type gene amplification system includes separate light sources for each step of a polymerase chain reaction in order to perform a high-speed temperature cycle by moving a rotation unit, in which a plurality of sample chambers are formed, to places at which the sample chambers are controlled at the temperatures corresponding to each step.
摘要翻译： 公开了一种非接触式加热型基因扩增系统。 非接触加热型基因扩增系统包括用于聚合酶链式反应的每个步骤的分开的光源，以通过将形成有多个样品室的旋转单元移动到高速温度循环来执行高速温度循环 其中样品室被控制在对应于每个步骤的温度。
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27.发明公开System for processing and/or analyzing liquid samples, sealing arrangement and method for heat-sealing a microplate 审中-公开系统，用于处理和/或液体样品的分析，密封装置和方法，用于热封微孔板
公开(公告)号：EP2446967A1
公开(公告)日：2012-05-02
申请号：EP10189647.0
申请日：2010-11-02
发明人： Schlaubitz, Thomas , Studer, Pius
CPC分类号： B01L3/50853 , B01L2200/0689 , B01L2200/12 , B01L2200/141 , B01L2300/0829 , B01L2300/168 , B01L2300/1811 , B01L2300/1827 , B01L2300/1861 , B01L2300/1872
摘要： The present invention pertains to a system for processing involving pipetting and/or analyzing liquid samples comprising a sealing arrangement for heat-sealing a microplate (28) having an upper face provided with a plurality of open-top wells (5), comprising a pressing element (8) provided with a pressing face for pressing a thermally fixable sealing cover (9) for sealing said wells on said microplate and a plurality of heat releasing sections (12) located in a manner to release heat to said microplate-pressed sealing cover except for foil regions covering openings of said wells. It further relates to a method for heat-sealing a microplate provided with a plurality of wells, comprising the following steps of: disposing a thermally fixable sealing cover over said wells; pressing said sealing cover on said microplate by means of a pressing element; and releasing heat to said sealing cover except for regions covering openings of said wells so as to thermally fix said sealing cover to said microplate.
摘要翻译： 本发明涉及一种系统，用于处理涉及移液和/或分析包括用于密封装置的液体样品的热封具有上设置有开顶孔的多个（5）上表面上的微板（28）包括一个按压 用于按压热可固定密封罩（9），用于密封位于的方式释放热量到所述微板压密封罩在所述微量培养板和热释放部分的多个（12）所述的孔设有一推压面元件（8） 除了用于覆盖所述孔的开口箔的区域。 本发明还涉及一种方法热封设置有孔的多个微板中，包括以下步骤：设置在所述孔中的导热可固定密封盖; 所述按压在所述微量培养板密封盖通过按压元件的装置; 和释放热到所述密封罩除了用于覆盖所述孔的开口区域，以固定热所述密封罩在所述微量培养板。
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28.发明公开Method and apparatus for amplifying nucleic acid sequences 审中-公开Verfahren und Vorrichtung zur Amplifikation vonNukleinsäuresequenzen
公开(公告)号：EP2353716A1
公开(公告)日：2011-08-10
申请号：EP10001234.3
申请日：2010-02-05
发明人： Braun, Dieter. , Mast, Christof
CPC分类号： B01L3/502715 , B01L3/5027 , B01L3/50273 , B01L7/525 , B01L2200/0631 , B01L2300/0816 , B01L2300/0838 , B01L2300/1822 , B01L2300/1872 , B01L2400/0445 , B01L2400/0451 , B01L2400/0454 , C12Q1/686 , C12Q2527/15 , C12Q2527/101
摘要： A method of amplifying one or more target nucleic acid sequence(s) in a PCR buffer solution comprising one or more target nucleic acid sequence(s), a nucleic acid polymerase, NTPs, at least a first primer and a second primer is provided. The PCR buffer solution is contained in an elongated vessel (2). The solution within the vessel (2) is locally heated in a spatio-temporally varying manner. Fluid flows (31-34) circulating within the vessel are established. At least a fraction of the PCR buffer solution cyclically travels between regions (35, 36; 37, 38) having different temperatures, giving rise to a temperature cycling.
摘要翻译： 提供了在包含一个或多个靶核酸序列，核酸聚合酶，NTPs，至少第一引物和第二引物的PCR缓冲溶液中扩增一个或多个靶核酸序列的方法。 PCR缓冲溶液包含在细长的容器（2）中。 容器（2）中的溶液以时空变化的方式局部加热。 确定在船内循环的流体流（31-34）。 至少一部分PCR缓冲液循环地在具有不同温度的区域（35,36; 37,38）之间行进，产生温度循环。
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公开(公告)号：EP2046940A4
公开(公告)日：2011-08-10
申请号：EP07809900
申请日：2007-06-26
发明人： HIRANO KIRK M , BABCOKE JASON E , CARRILLO ALBERT L , HAGA DOUWE D , KAO PIN , KINNEY PATRICK D , NURSE JAMES C
IPC分类号： C12M1/02
CPC分类号： B01L7/52 , B01L3/50851 , B01L3/50853 , B01L3/50857 , B01L7/00 , B01L2200/0605 , B01L2200/0642 , B01L2300/0654 , B01L2300/0829 , B01L2300/1816 , B01L2300/1838 , B01L2300/1861 , B01L2300/1872 , F28F13/00 , F28F2013/001 , G01N21/0332 , G01N21/6452 , G01N2201/062 , H05B3/84 , H05B6/02 , H05B6/105
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公开(公告)号：EP1513949B1
公开(公告)日：2010-04-28
申请号：EP03722089.4
申请日：2003-05-30
IPC分类号： C12Q1/68
CPC分类号： B04B15/02 , B01L7/52 , B01L2200/147 , B01L2300/0803 , B01L2300/1844 , B01L2300/1872 , C12Q1/686 , C12Q2563/173
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