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公开(公告)号：EP1268765B1
公开(公告)日：2008-08-13
申请号：EP01907187.7
申请日：2001-02-12
发明人： NICOLAIDES, Nicholas, C. , SASS, Philip, M. , GRASSO, Luigi , VOGELSTEIN, Bert , KINZLER, Kenneth, W.
CPC分类号： C12N15/1024 , C12N15/01 , C12N15/102
摘要： Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficinecy and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch reparir activity to the bacteria.
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公开(公告)号：EP1282708B1
公开(公告)日：2008-07-02
申请号：EP00989525.1
申请日：2000-12-28
发明人： NICOLAIDES, Nicholas C. , GRASSO, Luigi , SASS, Philip M. , KINZLER, Kenneth , VOGELSTEIN, Bert c/o Molecular Genetics Laboratory
CPC分类号： C12N15/1024 , C07K14/415 , C12N15/8216 , C12N15/8241
摘要： Blockade of mismatch repair in a plant can lead to hypermutation and a new genotype and/or phenotype. One approach used to generate hypermutable plants is through the expression of dominant negative alleles of mismatch repair genes in transgenic plants or derived cells. By introducing these genes into cells and transgenic plants, new cell lines and plant varieties with novel and useful properties can be prepared more efficiently than by relying on the natural rate of mutation. Moreover, methods to inhibit the expression and activity of endogenous plant MMR genes and their encoded products are also useful to generate hypermutable plants.
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33.发明公开
公开(公告)号：EP1885860A2
公开(公告)日：2008-02-13
申请号：EP06759855.7
申请日：2006-05-15
申请人： Morphotek, Inc.
CPC分类号： C12N15/65 , C12N15/1034 , C12N15/85 , C12N2840/20 , C12N2840/203
摘要： The present invention relates to expression vectors containing nucleic acid sequences encoding one or more proteins of interest linked to one or more selection markers that can be used to select cells null for such vector and to such null cells.
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公开(公告)号：EP1861425A2
公开(公告)日：2007-12-05
申请号：EP06737748.1
申请日：2006-03-09
申请人： Morphotek, Inc.
IPC分类号： C07K16/30 , A61P35/00 , A61K39/395
CPC分类号： C07K16/28 , A61K39/3955 , A61K45/06 , A61K2039/505 , C07K16/30 , C07K16/3069 , C07K2317/56 , C07K2317/732 , C07K2317/77
摘要： This invention relates to the use of monoclonal and polyclonal antibodies that specifically bind to and become internalized by mesothelin-positive cells and also induce an immune effector activity such as antibody dependent cellular cytotoxicity. The antibodies are useful in specific delivery of pharmacologic agents to mesotlielin expressing cells as well as eliciting an immune- effector activity particularly on tumor cells and precursors. The invention is also related to cells expressing the monoclonal antibodies, polyclonal antibodies, antibody derivatives, such as human, humanized, and chimeric monoclonal antibodies, antibody fragments, mammalian cells expressing the monoclonal antibodies, derivatives and fragments, and methods of treating cancer using the antibodies, derivatives and fragments.
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35.发明公开USE OF ENDOSIALIN BINDING PROTEINS TO ISOLATE ENDOSIALIN POSITIVE CELLS 有权使用ENDOSIALINBINDENDEN蛋白隔离ENDOSIALINPOSITIVER细胞
公开(公告)号：EP1819736A2
公开(公告)日：2007-08-22
申请号：EP05847825.6
申请日：2005-12-02
申请人： Morphotek, Inc.
CPC分类号： G01N33/574 , C07K16/2851 , G01N33/56966 , G01N33/57492 , G01N2333/515 , G01N2333/70596 , G01N2500/00 , G01N2800/10 , G01N2800/16 , G01N2800/24
摘要： Monoclonal antibodies and other proteins that specifically bind to cells positive for endosialin are provided. The antibodies and proteins are useful in the isolation of endosialin-positive cells, particularly cells associated with neovascularization associated with cancer and neovascular disease. The invention is also related to cells that are isolated using monoclonal antibodies; antibody derivatives, such as chimeric and humanized monoclonal antibodies; antibody fragments; mammalian cells expressing the monoclonal antibodies, derivatives and fragment; and methods of detecting and isolation endosialin-positive cells using the antibodies, derivatives and fragments.
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36.发明公开REGULATED VECTORS FOR CONTROLLING DNA HYPERMUTABILITY IN EUKARIOTIC CELLS 有权REGULATED VECTORS FOR CONTROLLING在真核细胞中的DNA超突变
公开(公告)号：EP1476577A2
公开(公告)日：2004-11-17
申请号：EP03733833.2
申请日：2003-02-21
申请人： Morphotek Inc.
CPC分类号： C12N15/1024 , C12N15/85 , C12N2830/42 , C12N2840/203
摘要： The present invention features mammalian expression vectors that are useful for controlling DNA hypermutability in mammalian cell as well as the encoding polynucleotide sequences of vector sequences. In related aspects the invention features expression vectors and host cells comprising such polynucleotides. In other related aspects, the invention features transgenic cells expressing a mutator gene to enhance genome-wide mutagenesis, due to, for example, the presence of an exogenous mutator-encoding polynucleotide sequence. Further, the invention provides methods for using vector sequences that can remove the expression of such gene to restore DNA stability in a host cell.
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37.发明公开
公开(公告)号：EP1430123A2
公开(公告)日：2004-06-23
申请号：EP01935403.4
申请日：2001-05-14
申请人： The Johns Hopkins University , Morphotek Inc. , Nicolaides, Nicholas, C. , Sass, Philip, M. , Grasso, Luigi , Vogelstein, Bert , Kinzler, Kenneth
发明人： NICOLAIDES, Nicholas, C. , SASS, Philip, M. , GRASSO, Luigi , VOGELSTEIN, Bert , KINZLER, Kenneth, W.
IPC分类号： C12N15/10 , C12N15/01 , C12Q1/68 , A01K67/027
摘要： PROBLEM TO BE SOLVED: To provide a method for rendering cells hypermutable, and hypermutable cell lines altered genetically and phenotypically.SOLUTION: The invention relates to use of dominant negative alleles of human mismatch repair genes to generate hypermutable cells and organisms; the use of introducing genes into cells and transgenic animals by which new cell lines and animal varieties with new and useful properties can be prepared more efficiently than by relying on the natural rate of mutation; and use of mutagens for enhancing a rate of further augmented mutation.
摘要翻译： 要解决的问题：提供一种使细胞变性可变的方法，以及基因和表型改变的超可变细胞系。解决方案：本发明涉及人错配修复基因的显性阴性等位基因用于产生超可变细胞和生物体; 将基因导入细胞和转基因动物中，通过其可以比通过依赖于自然的突变率更有效地制备具有新的和有用的性质的新的细胞系和动物品种; 并使用诱变剂提高进一步增强突变率。
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38.发明公开METHODS FOR GENERATING ANTIBIOTIC RESISTANT MICROBES AND NOVEL ANTIBIOTICS 审中-公开用于生产抗生素具有耐药性的细菌和新型抗生素
公开(公告)号：EP1417329A1
公开(公告)日：2004-05-12
申请号：EP01963754.5
申请日：2001-07-25
申请人： Morphotek Inc.
CPC分类号： C12N15/10 , C12N15/102 , C12Q1/20
摘要： The invention provides methods for generating antibiotic resistant bacteria comprising blocking mismatch repair in a bacterium to make hypermutable bacteria, contacting the bacteria with at least one antibiotic, selecting bacteria that are resistant to the antibiotic, and culturing the antibiotic resistant bacteria. The invention also provides methods of determining the genes responsible for antibiotic resistance.
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39.发明授权METHODS FOR ISOLATING NOVEL ANTIMICROBIAL AGENTS FROM HYPERMUTABLE MAMMALIAN CELLS 有权法新的抗菌剂从高突变哺乳动物细胞的分离
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公开(公告)号：EP1861425B1
公开(公告)日：2012-05-16
申请号：EP06737748.1
申请日：2006-03-09
申请人： Morphotek, Inc.
IPC分类号： C07K16/30 , A61P35/00 , A61K39/395
CPC分类号： C07K16/28 , A61K39/3955 , A61K45/06 , A61K2039/505 , C07K16/30 , C07K16/3069 , C07K2317/56 , C07K2317/732 , C07K2317/77
摘要： This invention relates to the use of monoclonal and polyclonal antibodies that specifically bind to and become internalized by mesothelin-positive cells and also induce an immune effector activity such as antibody dependent cellular cytotoxicity. The antibodies are useful in specific delivery of pharmacologic agents to mesotlielin expressing cells as well as eliciting an immune- effector activity particularly on tumor cells and precursors. The invention is also related to cells expressing the monoclonal antibodies, polyclonal antibodies, antibody derivatives, such as human, humanized, and chimeric monoclonal antibodies, antibody fragments, mammalian cells expressing the monoclonal antibodies, derivatives and fragments, and methods of treating cancer using the antibodies, derivatives and fragments.
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