公开(公告)号：EP1025212B1

公开(公告)日：2007-09-26

申请号：EP98953487.0

申请日：1998-10-14

申请人： DARWIN MOLECULAR CORPORATION

发明人： BLACK, Margaret, E.

IPC分类号： C12N9/12 ,  C12N9/52 ,  C12N15/00 ,  C12N15/10 ,  C12N15/62 ,  C12N15/86 ,  C12N5/10 ,  A61K48/00

CPC分类号： C12N9/503 ,  A61K38/00 ,  A61K48/00 ,  C07K2319/00 ,  C12N9/1211 ,  C12N9/1229 ,  C12N2799/022 ,  C12N2799/028

摘要： The present invention provides isolated nucleic acid molecules encoding a Herpesviridae thymidine kinase enzyme comprising one or more mutations, at least one of the mutations encoding an amino acid substitution located toward the N-terminus from a DRH nucleoside binding site which increases a biological activity of the thymidine kinase, as compared to unmutated thymidine kinase. Such mutations include amino acid substitutions within a Q substrate binding domain which increases a biological activity of the thymidine kinase, as compared to unmutated thymidine kinase. Within a further aspect, fusion proteins are provided which have both guanylate kinase and thymidine kinase biological properties. Also provided are vectors suitable for expressing such DNA molecules, as well as methods for utilizing such vectors.

公开(公告)号：EP1419259A2

公开(公告)日：2004-05-19

申请号：EP02764846.8

申请日：2002-08-13

申请人： Aescu-Life GmbH ,  Eul, Joachim

发明人： EUL, Joachim

IPC分类号： C12N15/63 ,  C12Q1/68

CPC分类号： C12N9/1211 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1027

摘要： The invention relates to methods and products for repairing DNA with genetic defects in the area of a mutant exon, for the programmed death of tumour cells as well as for identifying naturally trans-spliced RNA, whereby said methods are based on the use of RNA spliced components, produced naturally in the cell.

公开(公告)号：EP0434721B1

公开(公告)日：2000-03-15

申请号：EP89910146.3

申请日：1989-09-13

申请人： MERIAL

发明人： GRIFFIN, Annette, Mary Inst.for Animal Health Ltd. ,  ROSS, Louis, J.N. Institute for Animal Health Ltd. ,  SCOTT, Simon, David Inst.for Animal Health Ltd. ,  BINNS, Matthew, M. Inst.for Animal Health Ltd.

IPC分类号： C12N15/38 ,  C12N15/86 ,  A61K39/245 ,  A61K39/255

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K39/255 ,  A61K2039/51 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N9/0093 ,  C12N9/1211 ,  C12N15/00 ,  C12N2710/16022 ,  C12N2710/16034 ,  C12N2710/16043 ,  C12N2710/16322 ,  C12N2710/16334 ,  C12N2710/16343

摘要： Various genes of herpes virus of turkeys (HVT), Marek's disease virus (MDV) and infectious laryngotracheitis virus (ILTV) have been identified as non-essential regions (and candidates for insertion sites for foreign genes) and/or as antigen-encoding regions. The former include the HVT homologue of the HSV (herpes simplex virus) gC gene, the TK (thymidine kinase) region of MDV or ILTV, ORF3 of ILTV (as defined herein), the ribonucleotide reductase (large subunit) gene of ILTV, MDV or HVT and the ribonucleotide reductase (small subunit) gene of MDV. The antigen-encoding regions include the HVT homologues of the HSV gB, gC and gH genes, the ILTV homologue of HSV gB, ORF2 of ILTV, and the HVT homologue of the HSV-1 immediate early genes IE-175 and IE-68. Manipulation of these genes allows vaccines to be prepared comprising attenuated virus or virus carrying heterologous antigen-encoding sequences.

公开(公告)号：EP0908517A3

公开(公告)日：2000-03-01

申请号：EP98121028.9

申请日：1989-09-13

申请人： MERIAL

发明人： Ross, Louis Joseph Norman ,  Scott, Simon David ,  Binns, Matthew McKinley

IPC分类号： C12N15/38 ,  C12N15/86 ,  C07K14/055

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K39/255 ,  A61K2039/51 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N9/0093 ,  C12N9/1211 ,  C12N15/00 ,  C12N2710/16022 ,  C12N2710/16034 ,  C12N2710/16043 ,  C12N2710/16322 ,  C12N2710/16334 ,  C12N2710/16343

摘要： A vaccine effective against Marek's disease virus (MDV) comprises (a) an MDV attenuated by virtue of being TK - or (b) a host expressing an MDV antigen, namely the respective MDV homologues of the HSV gB, gC, gD or gH glycoproteins (or antigenic parts thereof) or the respective MDV homologues of the HSV-1 immediate early genes IE-68 or IE-175. The host may be a herpes virus of turkeys (HVT), more particularly HVT in which the MDV antigen is inserted in the HVT homologue of the HSV gC gene, the ribonucleotide reductase (large subunit) gene or the thymidine kinase (TK) gene.

公开(公告)号：EP0974659A3

公开(公告)日：2000-02-23

申请号：EP99114400.7

申请日：1989-09-13

申请人： MERIAL

发明人： Griffin, Annette Mary ,  Ross, Louis Joseph Norman ,  Scott, Simon David ,  Binns, Matthew McKinley

IPC分类号： C12N15/38 ,  C07K14/03 ,  C12N15/86 ,  A61K39/245

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K39/255 ,  A61K2039/51 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N9/0093 ,  C12N9/1211 ,  C12N15/00 ,  C12N2710/16022 ,  C12N2710/16034 ,  C12N2710/16043 ,  C12N2710/16322 ,  C12N2710/16334 ,  C12N2710/16343

摘要： Various genes of herpes virus of turkeys (HVT), Marek's disease virus (MDV) and infectious laryngotracheitis virus (ILTV) have been identified as non-essential regions (and candidates for insertion sites for foreign genes) and/or as antigen-encoding regions. The former include the HVT homologue of the HSV (herpes simplex virus) gC gene, the TK (thymidine kinase) region of MDV or ILTV, ORF3 of ILTV (as defined herein), the ribonucleotide reductase (large subunit) gene of ILTV, MDV or HVT and the ribonucleotide reductase (small subunit) gene of MDV. The antigen-encoding regions include the HVT homologues of the HSV gB, gC and gH genes, the ILTV homologue of HSV gB, ORF2 of ILTV, and the HVT homologue of the HSV-1 immediate early genes IE-175 and IE-68. Manipulation of these genes allows vaccines to be prepared comprising attenuated virus or virus carrying heterologous antigen-encoding sequences.

公开(公告)号：EP0748631A3

公开(公告)日：2000-02-23

申请号：EP96108830.9

申请日：1989-09-13

申请人： MERIAL

发明人： Ross, Louis Joseph Norman ,  Scott, Simon David ,  Binns, Matthew McKinley

IPC分类号： C12N15/38 ,  C12N15/86 ,  A61K39/255 ,  C07K14/055

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K39/255 ,  A61K2039/51 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N9/0093 ,  C12N9/1211 ,  C12N15/00 ,  C12N2710/16022 ,  C12N2710/16034 ,  C12N2710/16043 ,  C12N2710/16322 ,  C12N2710/16334 ,  C12N2710/16343

摘要： A vaccine effective against Marek's disease virus (MDV) comprises (a) an MDV attenuated by virtue of being TK - or (b) a host expressing an MDV antigen, namely the respective MDV homologues of the HSV gB, gC, gD or gH glycoproteins (or antigenic parts thereof) or the respective MDV homologues of the HSV-1 immediate early genes IE-68 or IE-175. The host may be a herpes virus of turkeys (HVT), more particularly HVT in which the MDV antigen is inserted in the HVT homologue of the HSV gC gene, the ribonucleotide reductase (large subunit) gene or the thymidine kinase (TK) gene.

公开(公告)号：EP0910406A4

公开(公告)日：2000-01-26

申请号：EP97915994

申请日：1997-02-14

申请人： HESKA CORP

发明人： HAANES ELIZABETH J ,  FRANK REXANN A

IPC分类号： C12N15/09 ,  A61K31/00 ,  A61K31/70 ,  A61K39/245 ,  A61P31/00 ,  A61P31/04 ,  A61P31/10 ,  A61P31/22 ,  A61P33/00 ,  A61P37/00 ,  C07K14/03 ,  C12N5/10 ,  C12N7/01 ,  C12N7/04 ,  C12N15/869 ,  C07K1/00 ,  C07K16/00 ,  C12N5/00 ,  C12N15/38

CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  C12N9/1211 ,  C12N2710/16721 ,  C12N2710/16722 ,  C12N2710/16743

公开(公告)号：EP0879288A1

公开(公告)日：1998-11-25

申请号：EP97902417.0

申请日：1997-01-31

申请人： RHONE-POULENC RORER S.A.

发明人： CROUZET, Jo[l ,  BLANCHE, Françis ,  COUDER, Michel ,  CAMERON, Béatrice

IPC分类号： C12N15 ,  A61K31 ,  A61K48 ,  A61P35 ,  C12N9 ,  A61K38

CPC分类号： C12N9/1211 ,  A01K2217/05 ,  A61K38/00

摘要： The present invention relates to a nucleic acid sequence characterized in that it is derived from the wild nucleic acid sequence coding for a thymidine kinase, said nucleic acid sequence having at least one mutation in the region corresponding to the ATP binding site and conveniently a second mutation in the N-terminal region and/or C-terminal region. It also relates to variants of the wild thymidine kinase and their uses in genic therapy.

公开(公告)号：EP0802801A2

公开(公告)日：1997-10-29

申请号：EP95944229.0

申请日：1995-12-22

申请人： CHIRON CORPORATION

发明人： BURROWS, Francis, J. ,  FONG, Timothy, C ,  POLO, John, M. ,  DUBENSKY, Thomas, W., Jr. ,  JOLLY, Douglas, J.

IPC分类号： C12N15 ,  A61K35 ,  A61K38 ,  A61K45 ,  A61K48 ,  A61P35 ,  A61P43 ,  C07K14 ,  C12N5 ,  C12N9

CPC分类号： C12N9/0069 ,  A61K38/00 ,  A61K48/00 ,  C07K14/57 ,  C07K14/705 ,  C07K14/745 ,  C07K14/8132 ,  C07K14/8146 ,  C12N9/1211 ,  C12N9/6491 ,  C12N2799/021 ,  C12N2799/027

摘要： The present invention provides compositions and methods for treatment of solid tumors with gene therapy utilizing recombinant viral vectors that express polypeptides which selectively initiate irreversible coagulation of blood in the tumor vasculature, inhibit tumor neovascularization, are capable of activating a non-toxic agent into a toxic agent within the tumor vascular wall causing destruction of the vascular bed and absorb or metabolize nutrients in the blood being supplied to the tumor. The production of these polypeptides by transduced cells in or adjacent to the blood vessels of the tumor result in the death of tumor cells.

公开(公告)号：EP0748631A2

公开(公告)日：1996-12-18

申请号：EP96108830.9

申请日：1989-09-13

申请人： RHONE-MERIEUX S.A.

发明人： Ross, Louis Joseph Norman ,  Scott, Simon David ,  Binns, Matthew McKinley

IPC分类号： A61K39/00

CPC分类号： C12N15/86 ,  A61K38/00 ,  A61K39/00 ,  A61K39/12 ,  A61K39/245 ,  A61K39/255 ,  A61K2039/51 ,  A61K2039/5254 ,  A61K2039/5256 ,  C07K14/005 ,  C12N9/0093 ,  C12N9/1211 ,  C12N15/00 ,  C12N2710/16022 ,  C12N2710/16034 ,  C12N2710/16043 ,  C12N2710/16322 ,  C12N2710/16334 ,  C12N2710/16343

摘要： A vaccine effective against Marek's disease virus (MDV) comprises (a) an MDV attenuated by virtue of being TK - or (b) a host expressing an MDV antigen, namely the respective MDV homologues of the HSV gB, gC, gD or gH glycoproteins (or antigenic parts thereof) or the respective MDV homologues of the HSV-1 immediate early genes IE-68 or IE-175. The host may be a herpes virus of turkeys (HVT), more particularly HVT in which the MDV antigen is inserted in the HVT homologue of the HSV gC gene, the ribonucleotide reductase (large subunit) gene or the thymidine kinase (TK) gene.

摘要翻译： 对马立克氏病病毒（MDV）有效的疫苗包括（a）通过TK或（b）表达MDV抗原的宿主，即HSV gB，gC，gD的相应MDV同源物或 gH糖蛋白（或其抗原部分）或HSV-1立即早期基因IE-68或IE-175的相应MDV同源物。 宿主可以是疱疹病毒（HVT），更具体地说，HVV，其中MDV抗原插入到HSV gC基因的HVT同源物，核糖核苷酸还原酶（大亚基）基因或胸苷激酶（TK）基因）中。