公开(公告)号：EP2230512A1

公开(公告)日：2010-09-22

申请号：EP10005326.3

申请日：1998-10-06

申请人： TRUSTEES OF TUFTS COLLEGE

发明人： Walt, David R. ,  DICKINSON, Todd A.

IPC分类号： G01N33/50 ,  G01N21/62 ,  G01N21/77 ,  G01N21/64 ,  C12Q1/00

CPC分类号： G01N21/6452 ,  B01J2219/00317 ,  B01J2219/00524 ,  B01J2219/00648 ,  B01J2219/00659 ,  C40B60/14 ,  G01N21/6428 ,  G01N21/7703 ,  G01N33/0031 ,  G01N33/54313 ,  G01N33/54373 ,  G01N33/54393 ,  G01N2021/6484 ,  G01N2021/7786

摘要： A microsphere-based analytic chemistry system is disclosed in which self-encoding microspheres having distinct characteristic optical response signatures to specific target analytes may be mixed together while the ability is retained to identify the sensor type and location of each sensor in a random dispersion of large numbers of such sensors in a sensor array using an optically interrogatable encoding scheme. An optical fiber bundle sensor is also disclosed in which individual microsphere sensors are disposed in microwells at a distal end of the fiber bundle and are optically coupled to discrete fibers or groups of fibers within the bundle. The identities of the individual sensors in the array are self-encoded by exposing the array to a reference analyte while illuminating the array with excitation light energy. A single sensor array may carry thousands of discrete sensing elements whose combined signal provides for substantial improvements in sensor detection limits, response and signal-to-noise ratios.

摘要翻译： 公开了一种基于微球的分析化学系统，其中具有针对特定目标分析物的不同特征光学响应特征的自编码微球可以混合在一起，同时保留能力以识别大尺度随机色散中的每个传感器的传感器类型和位置 使用光学可询问编码方案的传感器阵列中的这种传感器的数量。 还公开了一种光纤束传感器，其中单个微球传感器设置在纤维束的远端处的微孔中，并且光束耦合到束内的离散纤维或纤维组。 阵列中各个传感器的身份通过将阵列暴露于参考分析物而进行自编​​码，同时用激发光能照射阵列。 单个传感器阵列可以携带数千个离散感测元件，其组合信号提供传感器检测限，响应和信噪比方面的实质性改进。

公开(公告)号：EP1638689B1

公开(公告)日：2010-07-21

申请号：EP04751571.3

申请日：2004-05-05

申请人： Robodesign International, Inc.

发明人： GANZ, Brian, L. ,  MICKLEY, Mandel, W. ,  MOULDS, John, Andrew ,  BROVOLD, Christopher, T.

IPC分类号： B01L3/02 ,  B01L3/00 ,  G01N1/00 ,  G01N21/00 ,  G01N31/22 ,  G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N21/29 ,  G01N21/41 ,  B01J19/00

CPC分类号： B01L3/0244 ,  B01J19/0046 ,  B01J2219/00351 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00475 ,  B01J2219/00527 ,  B01J2219/00529 ,  B01J2219/00549 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01J2219/00695 ,  B01J2219/00698 ,  B01J2219/00722 ,  B01L3/0241 ,  B01L9/52 ,  C40B60/14 ,  C40B70/00 ,  G01N1/312 ,  G01N35/1074 ,  G01N2035/00158 ,  G01N2035/1034

摘要： A microarrayer for spotting solution onto a receiving surface in an automated microarray dispensing device. Elements of the invention include: at least one dispense head(6,40) for spotting the receiving surface (4A1-4E10), at least one light source (13) capable of illuminating the receiving surface, at least one camera (12) operating in conjunction with the at least one light source. The camera is capable of acquiring and transmitting surface image data to a computer. The computer is programmed to receive the surface image data and analyze it. The computer then generates post analysis data based on the analysis of the surface image data. The post analysis data is available for improving the spotting of the solution onto the receiving surface. In a preferred embodiment, the surface image data includes information relating to receiving surface alignment, information relating to spot quality, and receiving surface identification information. In a preferred, analysis of the information relating to receiving surface alignment enables the computer to make automatic adjustments to the relative positions of the at least one dispense head and the receiving surface to increase the accuracy of the spotting. In an embodiment, analysis of the information relating to spot quality identifies a spot as pass or fail.

公开(公告)号：EP2202520A1

公开(公告)日：2010-06-30

申请号：EP10000480.3

申请日：2001-11-27

申请人： Intelligent Medical Devices LLC

发明人： Jacobs, Alice Anne ,  Nicolic, Boris ,  Gupta, Vineet

IPC分类号： G01N33/543 ,  C12Q1/68 ,  G01N33/569

CPC分类号： B01F13/0088 ,  B01F13/08 ,  B01F13/0809 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00481 ,  B01J2219/00497 ,  B01J2219/00509 ,  B01J2219/00511 ,  B01J2219/00527 ,  B01J2219/00547 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00664 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/0074 ,  B01J2219/00745 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/5085 ,  B01L3/50857 ,  B01L3/563 ,  B01L2300/021 ,  B01L2300/0636 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2300/0864 ,  B82Y30/00 ,  C12Q1/6837 ,  C40B40/06 ,  C40B40/10 ,  C40B40/12 ,  C40B40/18 ,  C40B60/14 ,  C40B70/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/54366

摘要： The present invention is related to a method of determining a cause of one or more medical symptoms exhibited by a subject, the method comprising:
(a) obtaining a biological sample from the subject;
(b) obtaining an array of different probes or different sets of probes, wherein each probe or set of probes selectively interacts with a target associated with a different known cause of the one or more medical symptoms;
(c) applying the biological sample to the probes in the array under conditions that enable all of the probes to selectively interact with any targets in the biological sample;
(d) detecting interactions; and
(e) analyzing interactions to determine a cause of the one or more medical symptoms.

摘要翻译： 本发明涉及一种确定受试者表现出的一种或多种医学症状的原因的方法，所述方法包括：（a）从受试者获得生物样品; （b）获得不同探针或不同组探针的阵列，其中每个探针或探针组选择性地与与所述一种或多种医学症状的不同已知原因相关联的靶标相互作用; （c）在允许所有探针选择性地与生物样品中的任何靶标相互作用的条件下将生物样品施加到阵列中的探针; （d）检测相互作用; 和（e）分析相互作用以确定一种或多种医学症状的原因。

公开(公告)号：EP1978110B1

公开(公告)日：2010-05-26

申请号：EP08009183.8

申请日：2001-09-04

申请人： Transnetyx, Inc. ,  Hodge, Timothy A.

发明人： Hodge, Timothy A.

IPC分类号： C12Q1/68 ,  G06F19/00

CPC分类号： G01N35/00871 ,  B01J2219/00387 ,  B01J2219/00533 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6834 ,  C12Q1/6837 ,  C12Q1/6888 ,  C40B40/06 ,  C40B60/14 ,  G01N1/312 ,  G01N35/025 ,  G06F19/28 ,  G06Q50/24 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/143333 ,  C12Q2565/518

公开(公告)号：EP1718411B1

公开(公告)日：2010-04-07

申请号：EP05701382.3

申请日：2005-02-08

申请人： PamGene B.V.

发明人： VAN, BEUNINGEN, Marinus, Gerardus, Johannes

IPC分类号： B01L3/02

CPC分类号： B01L3/0275 ,  B01J2219/00292 ,  B01J2219/00639 ,  B01J2219/00659 ,  B01L2300/0636 ,  C40B60/14 ,  G01N2035/1055

公开(公告)号：EP2160237A2

公开(公告)日：2010-03-10

申请号：EP08727236.5

申请日：2008-03-31

申请人： Symyx Solutions Inc.

发明人： DIAMOND, Gary, M. ,  HALL, Keith, A. ,  McWAID, Thomas, Harding ,  MURPHY, Vince ,  MYSLOVATY, Michael ,  SHOEMAKER, James, A., W. ,  SIDHU, Robbie, Singh ,  TURNER, Howard ,  DIAS, Eric ,  SPITKOVSKY, Mikhail

IPC分类号： B01J19/00 ,  C40B60/14

CPC分类号： C40B60/14 ,  B01J19/0046 ,  B01J2219/00283 ,  B01J2219/00308 ,  B01J2219/00389 ,  B01J2219/00477 ,  B01J2219/00481 ,  B01J2219/00495 ,  B01J2219/00585 ,  B01J2219/00599 ,  B01J2219/00601 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00695 ,  B01J2219/00698 ,  B01J2219/0072 ,  B01J2219/00736 ,  F16K27/003

摘要： In one embodiment, a parallel batch reactor for effecting chemical reactions includes a vessel block (24) comprising reactor vessels (32) for receiving components of a reaction and a valve block (23) removably attached to the vessel block. The valve block (22) includes a first plurality of valves (72) in fluid communication with an inlet port for supplying pressurized fluid to the reactor vessels. (32) and configured to fluidically isolate one or more of the reactor vessels from (32) at least one of the other reactor vessels (32) The valve block (22) further includes a second plurality of valves (76) in fluid communication with the reactor vessels for injecting chemical components into the pressurized reactor vessels (32) or sampling chemical components from the pressurized reactor vessels (32) The vessel block (26) and valve block (22) are configured to sustain an operating pressure of at least 15 psig. A stirring assembly (26) may also be present.

公开(公告)号：EP1517753B1

公开(公告)日：2009-12-30

申请号：EP03748087.8

申请日：2003-09-25

申请人： Micronas Holding GmbH ,  Klapproth, Holger, Dr.

发明人： KLAPPROTH, Holger, Dr. ,  SIEBEN, Ulrich, Dr. rer. nat.

IPC分类号： B01L3/00 ,  B01J19/00

CPC分类号： G01N21/6454 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00353 ,  B01J2219/00511 ,  B01J2219/0052 ,  B01J2219/00522 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00734 ,  B01J2219/0074 ,  B01L3/5027 ,  B01L2300/0838 ,  B01L2400/0418 ,  B82Y30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N21/6428 ,  G01N2021/6439 ,  G01N2021/6482

摘要： The invention relates to a device (1) for determining the concentration of ligands (2) contained in a liquid sample. Said device comprises a capillary (3) having an inner cavity (4) filled with a liquid containing no ligands (2) or a smaller concentration of ligands than the sample. The capillary (3) has at least one opening (6) for bringing the liquid into contact with the sample. Receptors (5) are immobilised in the inner cavity (4) of the capillary (3), said receptors bonding with the ligands (2) on coming into contact with the same. The receptors (5) are distanced from the opening (6) and are connected thereto (6) by a diffusion path formed by the liquid. At least one sensor (7) is used to detect at least one measuring value representing the frequency of the ligand-receptor bonds. The invention device (1) enables the concentration of the ligands (2) in the sample to be precisely measured in a wide concentration range.

公开(公告)号：EP1470252B1

公开(公告)日：2009-12-09

申请号：EP02807744.4

申请日：2002-08-02

申请人： Centocor, Inc.

发明人： EVANS, Glen, A.

IPC分类号： C12Q1/68 ,  C12P19/34

CPC分类号： B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00659 ,  B01J2219/00691 ,  B01J2219/00695 ,  B01J2219/00722 ,  C12N15/10 ,  C12N15/66 ,  C40B40/06 ,  C40B60/14

公开(公告)号：EP1085943B1

公开(公告)日：2009-11-25

申请号：EP99923278.8

申请日：1999-05-20

申请人： Aventis Pharmaceuticals Inc.

发明人： STEIN,David T. ,  BAUM, Stephen, A.

IPC分类号： B01J19/00 ,  B01L3/00 ,  B01L11/00 ,  B01L9/00 ,  G01N33/53 ,  G01N33/543

CPC分类号： B82Y30/00 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/0031 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00322 ,  B01J2219/00461 ,  B01J2219/00468 ,  B01J2219/00475 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00502 ,  B01J2219/00522 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00668 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2300/0819 ,  B01L2300/0829 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  C40B60/14 ,  G01N33/543

摘要： A three-dimensional (3D) array of solid-phase supports is adapted to provide parallel synthesis of a library of molecules with 3D diversity. Individual locations in the 3D array may be assigned to selected molecules in the library such that molecules may be synthesized at and retrieved from such locations. Also, the supports include aperture walls in stacked plates; the supports may be suspended within stacked plate apertures; the 3D array includes discrete supports arranged in columns in one or more wells; the supports include tube inner walls or may be suspended in tubes, the tubes being secured in stacked, two-dimensional (2D) frameworks; or the supports include beads contained in porous enclosures having non-porous side walls and being secured in stacked, 2D frameworks. A support transfer device enables transfer of solid-phase supports used in a 3D array. Such apparatus includes: a rack of rods sized to be inserted through supports and a mechanism to prevent supports from coming off the rack; tubes connected to a vacuum manifold to suction supports one Z plane at a time; or a transfer block having recesses to receive one or more supports and at least one gate withholding supports from passing through the gate when in a closed position. A method of 3D synthesis includes: a) functionalizing solid-phase supports; b) placing supports in a 3D array; and c) performing parallel synthesis with 3D diversity. At least one unique R1 group member may be assigned to each Z plane.

公开(公告)号：EP1373566B1

公开(公告)日：2009-08-12

申请号：EP02707010.1

申请日：2002-03-28

申请人： Point-2-Point Genomics Ltd.

发明人： Hamill, Brendan

IPC分类号： C12Q1/68 ,  G06F9/00 ,  B01J19/00

CPC分类号： C12P19/34 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00527 ,  B01J2219/0054 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00659 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01L3/50851 ,  B01L7/52 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  C40B70/00 ,  C12Q2565/537 ,  C12Q2531/113

摘要： The invention comprises a two-step process for analysis of polynucleotides by chain extension of multiple oligonucleotide primers attached by 5'-linkages to solid supports by first performing PCR of the sample to be analysed in the presence of multiple oligonucleotides in solution, the nucleotide sequences of both sets of oligonucleotides being similar or identical. This produces immobilised single-stranded polynucleotides containing genetic sequence data derived from sample molecules. In a second step, support-bound polynucleotides are interrogated by hybridisation with a single labelled oligonucleotide probe or by second-strand synthesis with a primer-dependent polymerase using an oligonucleotide primer and nucleotide monomers, in which either or both of the primer and nucleotide monomers are labelled. Incorporation of label demonstrates the presence of two separate defined-sequence primers within the sample polynucleotide. The presence or absence within the sample of multiple combinations of primers are demonstrable in a single experiment by use of suitable apparatus, which may take the form of an oligonucleotide array. Multiple arrays may be accommodated in a 96-well format, allowing all possible combinations of 96 different primers to be generated simultaneously. If sequence data for the sample DNA is available in advance of the analysis, the results may be predicted by computer modelling, allowing design of highly-specific automated DNA-based assay procedures.