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公开(公告)号：EP0026176B1
公开(公告)日：1983-04-27
申请号：EP79901417.0
申请日：1979-03-19
IPC分类号： G01N33/54 , G01N33/56 , A61K39/395
CPC分类号： G01N33/532
摘要： Immunoassays are performed with two different ligands tagged with two different tagging constituents which are independent of each other and the two tagged ligands are immunologically bound together. The two different ligands may be detected independently through their independent tagging constituents for quality control, internal calibration (standardization), determination of viability and shelf life and the like.
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32.发明公开Separation of the free and antibody-bound ligand for labelled-ligand immuno-assays 失效用于标签免疫测定的免费和抗体绑定配偶的分离
公开(公告)号：EP0044988A3
公开(公告)日：1982-05-05
申请号：EP81105416
申请日：1981-07-11
发明人： Ziegler, Manfred, Dr. Dipl. Chem. , Woltanski, Klaus-Peter, Dipl.-Chem. , Diaz-Alonso, Jose, Manuel, Dipl.-Chem.
CPC分类号： G01N33/539 , G01N33/58
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公开(公告)号：EP0043272A1
公开(公告)日：1982-01-06
申请号：EP81302947.7
申请日：1981-06-29
CPC分类号： C07K14/005 , A61K39/00 , C07K16/1027 , C12N7/00 , C12N2760/18522 , C12N2760/18564 , C12N2760/18622 , Y02A50/489 , Y10S436/811
摘要： Antigens specific to respiratory syncytial virus are produced on the surface of cells by:
(1) culturing in vitro cells derived from a human or animal mucosa,
(2) inoculating the cultured cells with respiratory syncytial virus, and
(3) selecting virally infected cells from the culture.
The resulting cells or the viral antigen(s) when partially or completely isolated from the cells have immunological and diagnostic uses in respect of infection by respiratory syncytial virus and may be used to isolate viral antibodies. A specific cell strain NM7 produced by this method from bovine nasal mucosal cells has respiratory syncytial virus antigens on its surface and its corresponding, uninfected cell strain NM5 can be infected similarly.-
34.发明公开New unlabelled and radioactive labelled derivatives of butylamino propiophenone, their preparation, immunogens obtained from the unlabelled derivatives and a process for immunogen preparation, antisera raised using the immunogens and a method for raising antisera, a radioimmunoassay method and a test kit for use in the assay method 失效新的非特异性和放射性标记的丁基丙烯酸衍生物，其制备，免疫衍生物获得的免疫原和免疫原制备方法，使用免疫调节剂的抗生素和用于提高抗生素的方法，放射免疫测定方法和用于测定的试剂盒 方法
公开(公告)号：EP0038071A3
公开(公告)日：1981-12-23
申请号：EP81102833
申请日：1981-04-13
发明人： Welch, Richard Martin , Butz, Robert Frederick , Scharver, Jeffrey Douglas , Phillips, Arthur Page , Mehta, Nariman Bomanshaw , Findlay, John William Addison
IPC分类号： C07C97/10 , C07C131/00 , C07C103/52 , C07C102/04 , G01N33/56 , C07B23/00 , A61K47/00
CPC分类号： C07D263/10 , A61K47/646 , A61K51/0406 , C07C45/00 , C07C45/004 , C07C45/54 , C07C45/56 , C07C255/00 , C07D263/04 , G01N33/534 , Y10S436/804 , Y10S436/822 , Y10S530/806 , C07C49/82 , C07C49/825
摘要： New compounds are prepared having the formula:
wherein (1) X, is Cl, R 2 is oxygen and
R 1 is -(CH 2 ) n -OH or -(CH 2 ) n -O-(CO) m (CH 2 ) P COZ or -O-(CH 2 ) P COZ or -O-(CH 2 ) p COOR 11 or is a radioisotope or (2) X 1 is Cl, R' is Hand R' is = N . O(CH 2 ) q COZ or (3) X, is a radioisotope, R 2 is oxygen and R, is OH in the 4-position and wherein n is an integer of 1-5, m is 0 or 1, p is an integer of 1-4, q is an integer of 1-3, R 11 is a C 1 -C 3 alkyl group and Z is OH or a group of formula:
where s in an integer of 1-4, R 6 is a radioactive nuclide and R, is OH. Various methods for their preparation are described. The unlabelled compounds in which R, and R, contains a free carboxy group can be coupled with an immunogenic carrier to give an immunogen which can be used to raise antisera in host animals e.g. rabbits. This antisera contains antibodies specific to the antidepressant bupropion. This antisera can be used, together with the radioactive labelled compounds I in a radioimmunoassay method for determining bupropion in biological fluids as bupropion and the labelled analogue thereof compete for binding sites in the antisera. A test kit for use in the radioimmunoassay contains antisera, radioactive labelled compound I and optionally bupropion.摘要翻译： 制备具有下式的新化合物：其中（1）X1是Cl，R 2是氧，R 1是 - （CH 2）n -OH或 - （CH 2）n O-（CO）m（ CH2）pCOZ或-O-（CH2）pCOOR11或者是放射性同位素，或者（2）X1是Cl，R 1是H，R 2是= NO（CH 2）q COZ或 （3）X1为放射性同位素，R2为氧，R1为OH位，其中n为1-5的整数，m为0或1，p为1-4的整数，q为整数 为1-3，R11为C1-C3烷基，Z为OH或式：
其中s为1-4的整数，R6为放射性核素，R7为OH。 描述了各种制备方法。 其中R1和R2含有游离羧基的未标记的化合物可与免疫原性载体偶联，得到可用于在宿主动物中产生抗血清的免疫原， 兔子。 该抗血清含有对抗抑郁药物安非他酮有特异性的抗体。 该抗血清可以与放射免疫测定方法一起用于放射性标记的化合物I，用于测定生物液体中的安非他酮作为安非他酮，其标记的类似物与抗血清中的结合位点竞争。 用于放射免疫测定的测试试剂盒包含抗血清，放射性标记的化合物I和任选的安非他酮。 -
35.发明公开New unlabelled and radioactive labelled derivatives of butylamino propiophenone, their preparation, immunogens obtained from the unlabelled derivatives and a process for immunogen preparation, antisera raised using the immunogens and a method for raising antisera, a radioimmunoassay method and a test kit for use in the assay method 失效放射性标记的和未标记的Butylaminopropiophenon衍生物，其制备方法，从该未标记的衍生物的免疫原和抗血清的制备方法产生的，通过免疫原的抗血清和制备该抗血清，放射免疫测定法和Reagentiengarnitur用于本方法中使用的方法来生产。
公开(公告)号：EP0038071A2
公开(公告)日：1981-10-21
申请号：EP81102833.1
申请日：1981-04-13
发明人： Welch, Richard Martin , Butz, Robert Frederick , Scharver, Jeffrey Douglas , Phillips, Arthur Page , Mehta, Nariman Bomanshaw , Findlay, John William Addison
IPC分类号： C07C97/10 , C07C131/00 , C07C103/52 , C07C102/04 , G01N33/56 , C07B23/00 , A61K47/00
CPC分类号： C07D263/10 , A61K47/646 , A61K51/0406 , C07C45/00 , C07C45/004 , C07C45/54 , C07C45/56 , C07C255/00 , C07D263/04 , G01N33/534 , Y10S436/804 , Y10S436/822 , Y10S530/806 , C07C49/82 , C07C49/825
摘要： New compounds are prepared having the formula:
wherein (1) X, is Cl, R 2 is oxygen and
R 1 is -(CH 2 ) n -OH
or -(CH 2 ) n -O-(CO) m (CH 2 ) P COZ
or -O-(CH 2 ) P COZ
or -O-(CH 2 ) p COOR 11
or is a radioisotope
or (2) X 1 is Cl, R' is Hand R' is = N . O(CH 2 ) q COZ
or (3) X, is a radioisotope, R 2 is oxygen and R, is OH in the 4-position and wherein n is an integer of 1-5, m is 0 or 1, p is an integer of 1-4, q is an integer of 1-3, R 11 is a C 1 -C 3 alkyl group and Z is OH or a group of formula:
where s in an integer of 1-4, R 6 is a radioactive nuclide and R, is OH.
Various methods for their preparation are described. The unlabelled compounds in which R, and R, contains a free carboxy group can be coupled with an immunogenic carrier to give an immunogen which can be used to raise antisera in host animals e.g. rabbits. This antisera contains antibodies specific to the antidepressant bupropion. This antisera can be used, together with the radioactive labelled compounds I in a radioimmunoassay method for determining bupropion in biological fluids as bupropion and the labelled analogue thereof compete for binding sites in the antisera. A test kit for use in the radioimmunoassay contains antisera, radioactive labelled compound I and optionally bupropion.摘要翻译： 新的化合物制备具有下式：
worin（1）X1是Cl，R <2>是氧，R <1>是 - （CH 2）N-OH或 - （CH 2）n的O-（CO）米（ CH2）pCOZ或-O-（CH2）pCOZ或-O-（CH2）pCOOR11或是放射性同位素或（2）X1是Cl，R <1>为H，R <2>是= NO（CH 2）qCOZ或 （3）X1是放射性同位素，R 2是氧，R 1是OH在4位和worin n是1-5的整数，m是0或1; p是1-4的整数，q是一个整数 1-3，R 11是C1-C3烷基，Z是OH或一个式的基团： 其中以1-4整数s中，R 6是放射性核素和R 7为OH。 各种其制备方法进行了描述。 其中R1和R2含有游离羧基的未标记的化合物可以与在免疫原性载体被耦合以得到一种免疫原性哪个可以用于提高在宿主动物的抗血清E.G. 兔子。 该抗血清包含特定于抗抑郁药安非他酮抗体。 此抗血清可以用于连同放射性标记的化合物I在制备用于在生物流体如安非他酮确定性采矿安非他酮放射免疫测定方法及其标记类似物竞争结合在抗血清位点。 的检验试剂盒用于在放射免疫使用含有抗血清，放射性标记的式I化合物和任选的安非他酮。 -
36.发明公开Theophyllin-Antigene und -Antikörper, Verfahren zu deren Herstellung, ihre Verwendung zur Bestimmung von Theophyllin, Reagens-Set und Antiserum für diese Verwendung 无效茶碱抗原和抗体，它们的制备方法，其为茶碱，试剂盒及抗血清用于该用途的确定中使用。
公开(公告)号：EP0013910A1
公开(公告)日：1980-08-06
申请号：EP80100116.5
申请日：1980-01-11
IPC分类号： A61K39/385 , G01N33/56 , C07G7/00
CPC分类号： C07D473/08 , C07D311/54 , C07K16/00 , G01N33/531 , G01N33/94 , Y10S435/961 , Y10S435/975 , Y10S436/80 , Y10S436/804 , Y10S436/815 , Y10S530/806
摘要： Es wurde gefunden, daß sich zur Bestimmung von geringen Mengen Theophyllin in biologischen Flüssigkeiten einschließlich Vollblut und haemolytischem Blut nach der Methode des Immunoassay vorteilhaft Antikörper eignen, die durch Injizieren von Antigenen, bestehend aus kovalent über die Carboxylgruppe an immunogene Träger gebundenen Theophyllin-7-alkancarbonsäuren, in Wirtstiere zu erhalten sind. Als markiertes Theophyllin eignen sich insbesondere die mit radioaktiven Jod-Isotopen iodierten Theophyllin-7-alkancarbonsäureamide mit Histamin, Tyramin und Tyrosin, sowie enzym- und fluoreszenzmarkierte Theophyllinderivate.
摘要翻译： 已经发现适合用于少量生物流体中茶碱的包括全血和溶血的血液通过免疫测定有益的抗体的用由通过羧基免疫原性载体茶碱-7-烷羧酸共价键合抗原的注射引起的方法测定 ，可以在宿主动物来获得。 如特别是经标记的茶碱与放射性碘的同位素碘化茶碱-7- alkanecarboxamides是组胺，酪胺和酪氨酸，以及酶和荧光标记的茶碱。
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37.发明公开Stabilized platelet factor 4 immunoassay standards and radio-immunoassay method 失效一种稳定化的血小板因子4免疫测定标准溶液和放射免疫测定方法。
公开(公告)号：EP0007163A1
公开(公告)日：1980-01-23
申请号：EP79300880.6
申请日：1979-05-18
申请人： ABBOTT LABORATORIES
CPC分类号： G01N33/86 , G01N33/96 , G01N2400/40 , Y10S436/804 , Y10S436/826
摘要： The present invention encompasses a stabilized platelet factor4 immunoassay standard comprising an aqueous solution containing 10 to 100ng/ml of platelet factor 4, carrier protein, and an effective stabilizing amount of heparin, as well as 125 llabeled platelet factor 4 reagent stabilized in the same manner. These reagents are useful for testing blood plasma for activation of the coagulation system.
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38.发明公开
公开(公告)号：EP0101886A3
公开(公告)日：1986-07-02
申请号：EP83107087
申请日：1983-07-20
发明人： Kolb, Helmut, Prof. Dr. , Schleifer, Karl Heinz, Prof. Dr. , Seidl, Hans Peter, Dr. , Tympner, Klaus Dieter, Prof. Dr. , Weiss, Ludwig, Dr.
CPC分类号： G01N33/531 , G01N33/564 , G01N33/56911
摘要： Antikörper aus der Klasse der Immunglobuline G gegen das Peptiduntereinheit-Pentapeptid L-Ala-D-Glu(L-Lys-D-Ala- D-Ala) des Peptidoglycans in biologischem Untersuchungs material sowie gegen diese Peptidoglycane enthaltende bakterielle Zellwände, gekennzeichnet durch
a) spezifische Bindung an Pentapeptide der allgemeinen Formel
R - A₁ - D - Ala (I)
worin
R ein Aminosäureanteil mit 1 - 3, vorzugsweise 3 Aminosäuren aus der Gruppe Gly, L-Ala, D-Glu, L-Lys und
A₁ eine der Aminosäuren D-Ala, D-Ser, D-Val oder D-Leu bedeuten, oder
b) spezifische Bindung an Pentapeptide der allgemeinen Formel I enthaltende isolierte bakterielle Zellwände oder
c) spezifische Bindung an Pentapeptide der allgemeinen Formel I enthaltende grampositive Bakterien,
Verfahren zu ihrer Herstellung, Verfahren zu ihrer quanti tativen Bestimmung und Verwendung.-
公开(公告)号：EP0070033B1
公开(公告)日：1984-11-21
申请号：EP82106276.7
申请日：1982-07-13
发明人： Sato, Tomokazu , Ui, Michio
CPC分类号： G01N33/537 , G01N33/5308 , Y10S436/815
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公开(公告)号：EP0087465A4
公开(公告)日：1984-04-24
申请号：EP82902929
申请日：1982-08-12
申请人： UNIV JOHNS HOPKINS
发明人： STRAND DR METTE
摘要： Monoclonal antibodies to fluke glycoproteins have been developed for use in the immunodiagnosis of parasitic antigens in body fluids and for immunotherapy of fluke infection in warm blood animals.
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