摘要:
Provided herein are, inter alia, compositions and methods for identifying and using agents capable of inhibiting myofibroblast transition as well as methods for treating diseases associated with the same in a subject in need thereof.
摘要:
Antigen-specific T cells, including nave T cells, and including rare precursor cells are enriched and expanded in culture. Enrichment and expansion provides a platform for more effective immunotherapy by adoptive transfer, as well as platforms for personalizing immunotherapy by determining T cell reactivity with a library of candidate peptide antigens.
摘要:
Impressive responses have been observed in patients treated with checkpoint inhibitory anti-PD-1 or anti-CTLA-4 antibodies. However, immunotherapy against poorly immunogenic cancers remains a challenge. Treatment with both anti-PD-1 and anti-CTLA-4 antibodies were unable to eradicate large, modestly immunogenic CT26 tumors or metastatic 4T1 tumors. However, co-treatment with epigenetic modulating drugs and checkpoint inhibitors markedly improved treatment outcomes, curing more than 80% of them. Functional studies revealed that the primary targets of the epigenetic modulators were myeloid-derived suppressor cells (MDSCs). A PI3K-inhibitor that reduced circulating MDSCs also cured 80% of mice with metastatic 4T1 tumors when combined with immune checkpoint inhibitors. Thus, cancers resistant to immune checkpoint blockade can be cured by eliminating MDSCs.
摘要:
The presently disclosed subject matter provides compositions and methods for the expression of CRISPR guide RNAs using the H1 promoter. In particular, compositions and methods are provided for the use of the H1 promoter to express CRISPR guide RNA (gRNA) with altered specificity of the 5′ nucleotide, as well as use of the H1 promoter sequence as a bidirectional promoter to express Cas9 nuclease and the gRNA simultaneously. Compositions and methods are also provided for the expression and regulation of gRNA expression in vivo through the use of RNA ribozymes and regulatable aptazymes.
摘要:
The presently disclosed subject matter provides methods to treat cancer cells using low doses of DNA demethylating agents and poly ADP ribose polymerase (PARP) inhibitors. Methods also are provided for sensitizing a cell to a PARP inhibitor.
摘要:
The present invention is directed to a method for real-time characterization of spatially-resolved tissue optical properties using OCT/LCI. Imaging data are acquired, processed, displayed and stored in real-time. The resultant tissue optical properties are then used to determine the diagnostic threshold and to determine the OCT/LCI detection sensitivity and specificity. Color-coded optical property maps are constructed to provide direct visual cues for surgeons to differentiate tumor versus non-tumor tissue. These optical property maps can be overlaid with the structural imaging data and/or Doppler results for efficient data display. Finally, the imaging system can also be integrated with existing systems such as tracking and surgical microscopes. An aiming beam is generally provided for interventional guidance. For intraoperative use, a cap/spacer may also be provided to maintain the working distance of the probe, and also to provide biopsy capabilities. The method is usable for research and clinical diagnosis and/or interventional guidance.
摘要:
The present invention features compositions of Tmem100 peptides and variants thereof, and their use in treating or preventing diseases or conditions.
摘要:
The present invention is directed to a computer application for monitoring and tracking leg and foot movements and positions and a device for facilitating the computer tracking of the leg and foot movements. The application uses an accelerometer, gyroscope or other movement detectors in available devices such as a phone, movement tracker, personal music device, tablet computing device, other similar device or a device specifically designed to detect movements and positions and to track the movement and changes in position of the patient's leg and foot. The device can be held onto the patient's leg using a band type device that is easy to use and comfortable during sleep or by incorporation into a comfortable wearable band. The application includes a user interface and a backend for use by physicians or other healthcare staff to review and diagnose the patient's leg and foot movement patterns.