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    METHODS AND PROBES FOR DETECTION AND/OR QUANTIFICATION OF NUCLEIC ACID SEQUENCES
    42.
    发明公开
    METHODS AND PROBES FOR DETECTION AND/OR QUANTIFICATION OF NUCLEIC ACID SEQUENCES 审中-公开
    方法和探针检测和/或定量NUCLEIC序列的

    公开(公告)号：EP1356104A2

    公开(公告)日：2003-10-29

    申请号：EP01981456.5

    申请日：2001-10-09

    申请人： Nugen Technologies, Inc.

    发明人： KURN, Nurith

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q2537/143 ,  C12Q2537/101 ,  C12Q2525/313 ,  C12Q2565/101 ,  C12Q2537/1376 ,  C12Q2537/163

    摘要： The present invention discloses nucleic acid detector probes for specific detection and/or quantification of target nucleic acid sequences and detection and/or quantification methods using these probes. In the absence of target nucleic acid sequence, a first oligonucleotide and a third oligonucleotide are bound to each other in a conformation which brings two member of an interacting moiety pair (labels) into close spatial proximity. Cooperative binding of the first oligonucleotide and a second oligonucleotide to a target nucleic acid sequence causes displacement of the third oligonucleotide from the first oligonucleotide probe resulting in separation of the two members of the interacting moiety pair (labels). The spatial separation of the moieties (labels) is detectable, and indicates the presence and/or amount of the target nucleic acid sequence. The method is useful for detection and/or quantification of a specific nucleic acid sequence as well as the detection of sequence alteration in the target nucleic acid sequence.

    METHODS AND COMPOSITIONS FOR TRANSCRIPTION-BASED NUCLEIC ACID AMPLIFICATION
    43.
    发明公开
    METHODS AND COMPOSITIONS FOR TRANSCRIPTION-BASED NUCLEIC ACID AMPLIFICATION 有权
    NUCLEIC的有关转录作用的方法和组合物基于繁殖

    公开(公告)号：EP1356094A2

    公开(公告)日：2003-10-29

    申请号：EP01950624.5

    申请日：2001-06-26

    申请人： Nugen Technologies, Inc.

    发明人： KURN, Nurith

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6865 ,  C12Q1/6876 ,  C12Q2531/119 ,  C12Q2525/186 ,  C12Q2525/143

    摘要： Methods for isothermal exponential amplification of a target polynucleotide are disclosed. The methods employ two transcription modules, the first module providing linear amplification resulting in RNA transcripts, and a second module providing for further (generally cyclical) amplification resulting in more RNA transcripts. In one aspect, the amplification for the first module is composite primer based. In a second aspect, the amplification of the first module is based on target switching to generate a primer extension product comprising a promoter sequence. In all aspects, the RNA transcripts of the first transcription module are subjected to further amplification by creating an intermediate product comprising a double stranded promoter region from which transcription can occur. The invention further provides compositions and kits for practicing said methods, as well as methods which use the amplification results.

    SEQUENTIAL SEQUENCING
    44.
    发明公开
    SEQUENTIAL SEQUENCING 审中-公开
    连续测序

    公开(公告)号：EP2971130A4

    公开(公告)日：2016-10-05

    申请号：EP14764629

    申请日：2014-03-14

    申请人： NUGEN TECHNOLOGIES INC

    发明人： AMORESE DOUG ,  SCHROEDER BENJAMIN G ,  SCOLNICK JONATHAN

    IPC分类号： C12Q1/68 ,  G06F19/20 ,  G06F19/22

    CPC分类号： C12Q1/6874 ,  G06F19/22

    摘要： The present invention provides improved methods, compositions and kits for short read next generation sequencing (NGS). The methods, compositions and kits of the present invention enable phasing of two or more nucleic acid sequences in a sample, i.e. determining whether the nucleic acid sequences (typically comprising regions of sequence variation) are located on the same chromosome and/or the same chromosomal fragment. Phasing information is obtained by performing multiple, successive sequencing reactions from the same immobilized nucleic acid template. The methods, compositions and kits provided herein are useful, for example, for haplotyping, SNP phasing, or for determining downstream exons in RNA-seq.

    COMPOSITIONS AND METHODS FOR NEGATIVE SELECTION OF NON-DESIRED NUCLEIC ACID SEQUENCES
    46.
    发明公开
    COMPOSITIONS AND METHODS FOR NEGATIVE SELECTION OF NON-DESIRED NUCLEIC ACID SEQUENCES 有权
    组合物和不想要的序列NUCLEIC阴性选择方法

    公开(公告)号：EP2861787A2

    公开(公告)日：2015-04-22

    申请号：EP13806978.6

    申请日：2013-03-15

    申请人： Nugen Technologies, Inc.

    发明人： ARMOUR, Christopher ,  AMORESE, Doug ,  LI, Bin ,  KURN, Nurith

    IPC分类号： C40B30/04

    CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q1/6844 ,  C12Q2525/131 ,  C12Q2525/191 ,  C12Q2521/301 ,  C12Q2521/531 ,  C12Q2537/159

    摘要： The present invention provides methods, compositions and kits for the generation of next generation sequencing (NGS) libraries in which non-desired nucleic acid sequences have been depleted or substantially reduced. The methods, compositions and kits provided herein are useful, for example, for the production of libraries from total RNA with reduced ribosomal RNA and for the reduction of common mRNA species in expression profiling from mixed samples where the mRNAs of interest are present at low levels. The methods of the invention can be employed for the elimination of non-desired nucleic acid sequences in a sequence-specific manner, and consequently, for the enrichment of nucleic acid sequences of interest in a nucleic acid library.

    摘要翻译： 本发明提供了方法，组合物和试剂盒用于在所希望的非核酸序列已被耗尽或大幅减少下一代测序（NGS）库的生成。 在所提供的方法，组合物和试剂盒是有用的，例如，用于生产具有减少的核糖体RNA和常见的mRNA种类中表达从混合样品，其中所述感兴趣的mRNA以低水平存在仿形减少从总RNA库的 ， 本发明的方法可以被用于非核酸希望的序列在序列特异性方式消除，因此，对于所关注的核酸序列的核酸文库的富集。