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107 条记录 (耗时 0.018 秒)

    USE OF TRANS-ACTIVATION AND CIS-ACTIVATION TO MODULATE THE PERSISTENCE OF EXPRESSION OF A TRANSGENE
    51.
    发明公开
    USE OF TRANS-ACTIVATION AND CIS-ACTIVATION TO MODULATE THE PERSISTENCE OF EXPRESSION OF A TRANSGENE 有权
    反式激活和独联体活化TRANS的基因表达的持续时间调制

    公开(公告)号：EP1135515A2

    公开(公告)日：2001-09-26

    申请号：EP99964075.8

    申请日：1999-12-03

    申请人： GENVEC, INC.

    发明人： BROUGH, Douglas, E. ,  KOVESDI, Imre

    IPC分类号： C12N15/861 ,  C12N15/35 ,  C12N15/38 ,  C07K14/035 ,  C07K14/075 ,  C12N15/63

    CPC分类号： C12N15/86 ,  C07K14/005 ,  C12N15/63 ,  C12N2710/10322 ,  C12N2710/10345 ,  C12N2710/16622 ,  C12N2830/60 ,  C12N2840/60

    摘要： Provided are methods of modulating the persistence of the expression in a cell of a transgene, such as a transgene in a non-Herpes vector or in at least E4Δ adenoviral vector, and related systems. One method comprises contacting the cell with a non-Herpes vector comprising and expressing a gene encoding HSV ICP0, whereupon expression of HSV ICP0 the persistence of expression of the transgene is modulated. Further provided is a system for modulating the persistence of expression of a transgene, which system comprises a non-Herpes vector comprising (i) a gene encoding HSV ICP0 and (ii) a transgene, wherein the HSV ICP0 modulates the persistence of expression of the transgene and either the non-Herpes vector comprises the transgene or the system further comprises a vector, in which case the vector comprises the transgene. Another method comprises contacting the cell with an at least E4Δ adenoviral vector comprising (i) a transgene and (ii) a gene encoding a trans-acting factor, wherein the trans-acting factor modulates the persistence of expression of the transgene and the gene encoding the trans-acting factor is not from the E4 region of an adenovirus. Yet another method comprises contacting a cell simultaneously or sequentially with (i) an at least E4Δ adenoviral vector comprising a transgene and (ii) a viral vector comprising a gene encoding a trans-acting factor, which is not from the E4 region of an adenovirus and which modulates the persistence of expression of the transgene. Also provided is a system for modulating the persistence of expression of a transgene in an at least E4Δ adenoviral vector, which system comprises (i) an at least E4Δ adenoviral vector comprising a transgene and (ii) a gene encoding a trans-acting factor, wherein the gene encoding the trans-acting factor is not from the E4 region of an adenovirus, the trans-acting factor modulates the persistence of expression of the transgene, and either the at least E4Δ adenoviral vector comprises the gene encoding the trans-acting factor or the system comprises a viral vector, in which case the viral vector comprises the gene encoding the trans-acting factor.

    EFFICIENT PURIFICATION OF ADENOVIRUS
    52.
    发明公开
    EFFICIENT PURIFICATION OF ADENOVIRUS 有权
    高效清洗腺病毒

    公开(公告)号：EP1073721A1

    公开(公告)日：2001-02-07

    申请号：EP99918788.3

    申请日：1999-04-22

    申请人： GENVEC, INC.

    发明人： CARRI N, Miguel, E. ,  MENGER, Marilyn ,  KOVESDI, Imre

    IPC分类号： C12N7/02 ,  G01N33/569

    CPC分类号： C07K14/005 ,  B01D15/345 ,  B01D15/363 ,  B01J41/20 ,  C12N7/00 ,  C12N2710/10322 ,  C12N2710/10351 ,  C12Q1/06 ,  G01N30/06 ,  G01N30/96 ,  G01N2030/625 ,  G01N2030/8813 ,  G01N2333/075

    摘要： A method of enriching a solution of an adenovirus comprising applying a mixed solution comprising an adenovirus and at least one undesired type of biomolecule to an anion exchange chromatography resin containing a binding moiety selected from the group consisting of dimethylaminopropyl, dimethylaminobutyl, dimethylaminoisobutyl, and dimethylaminopentyl and eluting the adenovirus from the chromatography resin. Also provided is a method of purifying an adenovirus from adenovirus-infected cells comprising lysing such cells, applying the lysate to a single chromatography resin, eluting the adenovirus from the chromatography resin, and collecting a fraction containing adenovirus that is substantially as pure as triple CsC1 density gradient-purified adenovirus. The present method further provides a method of accurately quantifying the number of adenoviral particles in a solution of adenovirus comprising applying to and eluting from an anion exchange chromatography resin a sample solution of adenovirus, comparing the absorbance of the sample solution of adenovirus and the absorbance of a standard solution of adenovirus, and quantifying the number of adenoviral particles in the sample solution.

    ADENOVIRAL VECTOR-BASED RESPIRATORY SYNCYTIAL VIRUS (RSV) VACCINE
    58.
    发明公开
    ADENOVIRAL VECTOR-BASED RESPIRATORY SYNCYTIAL VIRUS (RSV) VACCINE 审中-公开
    AUF ADENOVIRENVEKTOREN-BASIERENDER IMPFSTOFF GEGEN DAS RESPIRATORISCHE SYNZYTIALVIRUS（RSV）

    公开(公告)号：EP2764011A2

    公开(公告)日：2014-08-13

    申请号：EP12773193.3

    申请日：2012-10-05

    申请人： GenVec, Inc.

    发明人： BROUGH, Douglas E. ,  GALL, Jason G.D. ,  MCVEY, Duncan

    IPC分类号： C07K14/005 ,  C12N15/86

    CPC分类号： C07K14/005 ,  C12N15/86 ,  C12N2710/10322 ,  C12N2710/10333 ,  C12N2710/10343 ,  C12N2760/18034 ,  C12N2799/022

    摘要： The invention provides an adenovirus or adenoviral vector characterized by comprising a nucleic acid sequence encoding one or more Respiratory Syncytial Virus (RSV) antigens and one or more particular nucleic acid sequences or one or more particular amino acid sequences, or portions thereof, pertaining to, for example, an adenoviral pIX protein, DNA polymerase protein, penton protein, hexon protein, and/or fiber protein, as well as a method of inducing an immune response against RSV in a mammal by administering the adenovirus or adenoviral vector to the mammal.

    摘要翻译： 本发明提供一种腺病毒或腺病毒载体，其特征在于包含编码一种或多种呼吸道合胞病毒（RSV）抗原和一种或多种特定核酸序列或一个或多个特定氨基酸序列或其部分的核酸序列， 例如腺病毒pIX蛋白，DNA聚合酶蛋白，戊酮蛋白，六邻体蛋白和/或纤维蛋白，以及通过向哺乳动物施用腺病毒或腺病毒载体而在哺乳动物中诱导针对RSV的免疫应答的方法。