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公开(公告)号：EP1929046B1
公开(公告)日：2012-07-11
申请号：EP06803269.7
申请日：2006-09-07
发明人： KURN, Nurith , WANG, Shenglong
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6865
摘要： The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA- containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
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52.发明公开METHODS, COMPOSITIONS, AND KITS FOR GENERATING NUCLEIC ACID PRODUCTS SUBSTANTIALLY FREE OF TEMPLATE NUCLEIC ACID 审中-公开方法，组合物和药盒基本上GENERATINGNUKLEINSÄUREMATRIZENFREIENNUCLEIC产品
公开(公告)号：EP2464738A1
公开(公告)日：2012-06-20
申请号：EP10808789.1
申请日：2010-08-12
发明人： KURN, Nurith , WANG, Shenglong
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6806 , C12Q1/6853 , C12Q2521/531 , C12Q2525/179 , C12Q2535/122 , C12Q2525/173 , C12Q2525/121 , C12Q2525/119 , C12Q2525/101
摘要： Methods, kits, and compositions which enable the generation of double stranded DNA products from various substrates such as low abundance RNAs or whole transcriptosomes are provided. The double stranded DNA products are suitable for downstream applications involving microarray analysis or sequencing.
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公开(公告)号：EP2247727A4
公开(公告)日：2011-08-03
申请号：EP09711405
申请日：2009-02-12
发明人： KURN NURITH
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2565/537 , C12Q2525/121 , C12Q2521/319 , C12Q2531/119
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54.发明公开METHODS FOR ANALYSIS OF NUCLEIC ACID METHYLATION STATUS AND METHODS FOR FRAGMENTATION, LABELING AND IMMOBILIZATION OF NUCLEIC ACIDS 审中-公开方法核酸分析甲基化和碎片化状态的方法，标志和核酸的固定化
公开(公告)号：EP1711591A4
公开(公告)日：2010-04-28
申请号：EP04815722
申请日：2004-12-29
发明人： KURN NURITH , DAFFORN GEOFFREY A
CPC分类号： C12Q1/6827 , C12P19/34 , C12Q2525/119 , C12Q2521/531
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55.发明授权METHODS FOR GENERATING DOUBLE STRANDED DNA COMPRISING A 3' SINGLE STRANDED PORTION AND USES OF THESE COMPLEXES FOR RECOMBINATION 有权用于产生双链DNA与单链3“共享与方法使用这个复杂的用于重组
公开(公告)号：EP1488001B1
公开(公告)日：2008-08-20
申请号：EP03717952.0
申请日：2003-03-11
CPC分类号： C12Q1/6853 , C12N15/1096 , C12Q2525/207 , C12Q2521/107 , C12Q2525/203
摘要： Methods of recombining nucleic acids are provided. In particular, methods for the production of partially double stranded nucleic acids comprising a 3’ overhang from an RNA target and use in methods of recombining polynucleotides is described. These methods do not require thermocycling. The present invention also provides methods of recombining and selection which allow for identification of proteins comprising improved or desired characteristics.
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公开(公告)号：EP1929046A2
公开(公告)日：2008-06-11
申请号：EP06803269.7
申请日：2006-09-07
发明人： KURN, Nurith , WANG, Shenglong
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6865
摘要： The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA- containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
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公开(公告)号：EP1390537A4
公开(公告)日：2006-04-26
申请号：EP02731119
申请日：2002-03-11
发明人： KURN NURITH
IPC分类号： G01N33/53 , C12N15/09 , C12N15/10 , C12Q1/68 , G01N37/00 , C07H21/02 , C07H21/04 , C12P19/34
CPC分类号： C12N15/1096 , C12Q1/6865 , C12Q2525/143
摘要： The invention provides methods for linear and exponential amplification of RNA. They are particularly suitable for amplifying a plurality of RNA species in a sample. The methods are based on hybridization of polynucleotide comprising a propromoter sequence to a primer extension product to generate an intermediate polynucleotide capable of driving transcription, whereby multiple copies of RNA products comprising sequences complementary to an RNA sequence of interest are generated. The methods are useful for preparation of nucleic acid libraries and substrates for analysis of gene expression of cells in biological samples. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
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58.发明公开SINGLE PRIMER ISOTHERMAL NUCLEIC ACID AMPLIFICATION-ENHANCED ANALYTE DETECTION AND QUANTIFICATION 审中-公开BY ISOTHERME单个引物，核酸扩增改进的检测和分析物的定量
公开(公告)号：EP1490514A2
公开(公告)日：2004-12-29
申请号：EP03718172.4
申请日：2003-03-31
CPC分类号： C12Q1/6804 , C12Q1/6865 , C12Q2563/179 , C12Q2525/143 , C12Q2525/121 , C12Q2521/319 , C12Q2537/1373 , C12Q2521/313
摘要： The present invention provides novel methods of indirect analyte detection and quantification through amplification of oligonucleotide template attached to binding partners for analytes by nucleic acid amplification utilizing isothermal, single primer linear nucleic acid amplification methods. Methods of binding of binding partner that is attached to an oligonucleotide template to analyte, then amplifying at least a portion of the oligonucleotide template using a composite primer, primer extension, strand displacement, and optionally a termination sequence, are provided. Methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter oligonucleotide and transcription are also provided. Methods for detecting and quantifying amplification products are also provided. The invention further provides compositions and kits for practicing said methods.
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59.发明公开METHODS FOR GENERATING DOUBLE STRANDED DNA COMPRISING A 3’ SINGLE STRANDED PORTION AND USES OF THESE COMPLEXES FOR RECOMBINATION 有权用于产生双链DNA与单链3“共享与方法使用这个复杂的用于重组
公开(公告)号：EP1488001A2
公开(公告)日：2004-12-22
申请号：EP03717952.0
申请日：2003-03-11
CPC分类号： C12Q1/6853 , C12N15/1096 , C12Q2525/207 , C12Q2521/107 , C12Q2525/203
摘要： Methods of recombining nucleic acids are provided. In particular, methods for the production of partially double stranded nucleic acids comprising a 3’ overhang from an RNA target and use in methods of recombining polynucleotides is described. These methods do not require thermocycling. The present invention also provides methods of recombining and selection which allow for identification of proteins comprising improved or desired characteristics.
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60.发明公开Composition for linear isothermal amplification of polynucleotide sequences 有权Zusammensetzung zur linearen isothermalen Amplifizierung von Polynukleotidsequenzen
公开(公告)号：EP1431303A2
公开(公告)日：2004-06-23
申请号：EP04002084.4
申请日：2000-09-13
发明人： Kurn, Nurith
CPC分类号： C12Q1/6844 , C12Q1/6865 , C12Q2531/101 , C12Q2537/1373 , C12Q2521/301 , C12Q2525/186 , C12Q2525/143 , C12Q2525/121 , C12Q2521/313
摘要： The present invention provides novel composite primers. The composite primers are suitable for use in isothermal, single primer linear nucleic acid amplification methods, such as methods for amplifying complementary DNA using the composite primer, primer extension, strand displacement, and optionally a termination sequence or methods for amplifying sense RNA using a composite primer, primer extension, strand displacement, optionally template switching, a propromoter olignucleotide and transcription. The invention further provides compositions and kits comprising the composite primers.
摘要翻译： 本发明提供新的复合引物。 复合引物适用于等温单引物线性核酸扩增方法，例如使用复合引物扩增互补DNA的方法，引物延伸，链置换和任选的终止序列或使用复合物扩增正义RNA的方法 引物，引物延伸，链置换，任选的模板切换，启动子寡核苷酸和转录。 本发明还提供了包含复合引物的组合物和试剂盒。
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