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    Peptide Conjugate useful for cell nucleus molecular imaging and tumor therapy
    65.
    发明公开
    Peptide Conjugate useful for cell nucleus molecular imaging and tumor therapy 审中-公开
    Peptidkonjugat zur molekularen Zellkernabbildung oder Tumorbehandlung

    公开(公告)号：EP1424343A1

    公开(公告)日：2004-06-02

    申请号：EP02026700.1

    申请日：2002-11-29

    申请人： Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts

    发明人： Heckl, Stefan ,  Braun, Dr. Klaus ,  Pipkorn, Dr. Rüdiger ,  Waldeck, Waldemar Dr.

    IPC分类号： C07K14/47 ,  A61K49/14 ,  A61K47/48 ,  A61P35/00

    CPC分类号： C12N15/62 ,  A61K47/64 ,  A61K49/085 ,  A61K49/14 ,  A61K51/088 ,  C07K19/00 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/09

    摘要： Described is a conjugate comprising (a) an amphiphilic transport peptide of human origin as a transmembrane module (TPU), (b) a nuclear localization sequence (NLS) and (c) a signalling and/or drug carrying module (SM), preferably comprising Gd, Ga, Fe, Mn, I and/or F as (diagnostic) image creating compound. Said conjugate is useful for diagnostic purposes, e.g., for cell tracking by MRI, as a contrast agent (e.g., replacing a "biopsy clip") for MRI, or for determining the activity of DNA repair enzymes by MRI. Said conjugate is also useful for therapy, e.g., for chemotherapy or intranuclear Gadolinium Neutron Capture Therapy (GNCT).
    The transmembrane module (TPU) is selected among peptides of human origin, whose amino acid sequences are similar to the sequence of the antennapedia fragment RQIKIWFQNRRMKWKK. In a specific embodiment, TPU is derived from the human homeobox protein HOX-B1. The nuclear localization sequence (NLS) is derived from the simian virus 40-T antigen or from a transcription factor.

    摘要翻译： 描述了包含（a）作为跨膜模块（TPU）的人源的两亲性转运肽，（b）核定位序列（NLS）和（c）信号传导和/或药物携带模块（SM））的两性转运肽 包括Gd，Ga，Fe，Mn，I和/或F作为（诊断）图像产生化合物。 所述缀合物可用于诊断目的，例如通过MRI进行细胞跟踪，作为造影剂（例如，替换“活检夹”）用于MRI，或用于通过MRI确定DNA修复酶的活性。 所述缀合物也可用于治疗，例如用于化疗或核内钆中子捕获治疗（GNCT）。 跨膜模块（TPU）选自人源的肽，其氨基酸序列类似于触角片片段RQIKIWFQNRRMKWKK的序列。 在具体实施方案中，TPU衍生自人同源盒蛋白HOX-B1。 核定位序列（NLS）源自猿猴病毒40-T抗原或转录因子。

    Screening method for the identification and characterization of DNA methyltransferase inhibitors
    67.
    发明公开
    Screening method for the identification and characterization of DNA methyltransferase inhibitors 审中-公开
    体内筛选 - 弗氏绒毛DNA甲基转移酶抑制剂

    公开(公告)号：EP1384787A1

    公开(公告)日：2004-01-28

    申请号：EP02016336.6

    申请日：2002-07-25

    申请人： Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts

    发明人： Lyko, Frank

    IPC分类号： C12Q1/48 ,  G01N33/50 ,  A01K67/033

    CPC分类号： G01N33/5085 ,  A01K67/0339 ,  A01K2217/05 ,  A01K2227/706 ,  A01K2267/0331 ,  C12N15/8509 ,  C12Q1/48 ,  G01N2520/00

    摘要： The present invention therefore provides a screening method for the identification and characterization of inhibitors of DNA methyltransferases comprising the following steps:

    a) generation of at least one Drosophila culture

    which culture comprises at least one offspring that is derived from a cross between flies of the Drosophila strains GawB(69B) and UAS-Dnmt3a and in an appropriate developmental stage,
    a sufficient amount of Drosophila medium
    and an appropriate concentration of at least one candidate compound for a DNA methyltransferase inhibitor,

    b) incubation of the the Drosophila culture of step a) under incubation conditions which allow normal Drosophila development for an appropriate incubation period,
    c) identification of those Drosophila cultures of step b) that show the presence of mobile larvae or pupae.

    In another aspect, the invention concerns the offsprings derived from a cross between the transgenic Drosophila strains GawB(69B) and UAS-Dnmt3a , as well as their use for the identification and characterization of inhibitors of DNA methyltransferases, particularly of inhibitors of Dnmt3a.

    摘要翻译： 因此，本发明提供了用于鉴定和表征DNA甲基转移酶抑制剂的筛选方法，其包括以下步骤：a）产生至少一种果蝇培养物，其培养物包含至少一种衍生自果蝇之间的杂交的后代 菌株GawB（69B）和UAS-Dnmt3a，并且在合适的发育阶段，足量的果蝇培养基和适合浓度的DNA甲基转移酶抑制剂的至少一种候选化合物，b）孵育步骤a）的果蝇培养物， 在允许正常果蝇发育适宜的孵育期的孵育条件下，c）鉴定显示存在移动幼虫或蛹的步骤b）的果蝇培养物。 另一方面，本发明涉及源自转基因果蝇菌株GawB（69B）和UAS-Dnmt3a之间的杂交的后代，以及它们用于鉴定和表征DNA甲基转移酶抑制剂，特别是抑制剂的用途 的Dnmt3a。

    KÄFIG-REGALSYSTEM MIT ZWANGSBELÜFTUNG
    69.
    发明授权
    KÄFIG-REGALSYSTEM MIT ZWANGSBELÜFTUNG 有权
    与强制通风笼架系统

    公开(公告)号：EP1303182B1

    公开(公告)日：2003-12-17

    申请号：EP01969446.2

    申请日：2001-07-20

    申请人： Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts

    发明人： ZILLMANN, Uwe ,  DÄHMEL, Werner ,  LEHR, Bernd

    IPC分类号： A01K1/03

    CPC分类号： A01K1/031

    摘要： The invention relates to a cage rack system (1) with forced ventilation. Each cage (2) in said system is connected to a ventilation unit (3) which is configured in such a way that the cages (2) can be flushed for ventilation in a low pressure mode or an overpressure mode. The aim of the invention is to provide a cage rack system (1) of this type that is more simple, more economical and more easy to handle and transport and which has a high level of operational reliability. To this end, the ventilation unit (3) has only one pump (4) with a drive (5) and is configured in such a way that a conveying direction (6 or 7) can be adjusted to or from the cages (2).

    Target genes for the diagnosis and treatment of cancer
    70.
    发明公开
    Target genes for the diagnosis and treatment of cancer 审中-公开
    Zielgenefürdie Diagnose und Behandlung von Krebs

    公开(公告)号：EP1369482A1

    公开(公告)日：2003-12-10

    申请号：EP02012705.6

    申请日：2002-06-07

    申请人： Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts

    发明人： Wittig, Rainer ,  Poustka, Annemarie ,  Mollenhauer, Jan ,  Schadendorf, Dirk

    IPC分类号： C12N15/12 ,  C07K14/47 ,  C07K16/18 ,  C12N5/10 ,  A61K31/00 ,  A61K38/00 ,  A61K48/00 ,  A61P35/00 ,  G01N33/53

    CPC分类号： C07K14/47

    摘要： The present invention relates to the identification and use of target genes for the detection and treatment of drug-resistant tumor cells. The nucleic acids of the present invention exhibit a deregulated phenotype when the tumor cells are subjected to cytostatic drugs, i.e. they are expressed in a higher or lower amount as compared to parental drug-sensitive cancer cells. Thus, they can be used as a diagnostic and pharmaceutical tool to render drug-resistant cells drug-sensitive. In addition, the present invention includes the polypeptides encoded by the respective nucleic acids, expression vectors harboring the nucleic acids, host cells for expression and methods for the diagnosis and treatment of drug-resistant tumor cells.

    摘要翻译： 本发明涉及用于检测和治疗耐药肿瘤细胞的靶基因的鉴定和应用。 当肿瘤细胞经受细胞抑制药物时，本发明的核酸表现出失调的表型，即与亲本药物敏感的癌细胞相比，它们以更高或更低的量表达。 因此，它们可以用作诊断和药物工具来使耐药细胞药物敏感。 此外，本发明包括由相应核酸编码的多肽，携带核酸的表达载体，用于表达的宿主细胞和用于诊断和治疗耐药性肿瘤细胞的方法。