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    Sex-specific insemination of mammals with low number of sperm cells
    63.
    发明公开
    Sex-specific insemination of mammals with low number of sperm cells 审中-公开
    Sex-spezifische Besamung derSäugetierenmit einem geringen Anzahl von Samenzellen

    公开(公告)号：EP2329710A2

    公开(公告)日：2011-06-08

    申请号：EP10185733.2

    申请日：1998-12-31

    申请人： XY, LLC

    发明人： Herickhoff, Lisa ,  Seidel, George E. ,  Schenk, John

    IPC分类号： A01K67/02

    CPC分类号： C12N5/0612 ,  A01K67/027 ,  A61D19/02 ,  C12N15/873 ,  G01N2015/149

    摘要： Artificial Insemination is achieved for sexed mammalian offspring in a commercially practical manner and with dosages of insemination sperm which were not previously thought to be practical for commercial implementation. Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid (3) and other strategies which minimize stress on the sperm cells (18), and potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems (14) and techniques for the process are describeb so that commercial application of sperm samples as well as the resulting animals may now be achieved in the field.

    摘要翻译： 以商业上实用的方式实现了性别哺乳动物后代的人工授精，并且以前认为对于商业实施并不实用的授精精子的剂量。 特别适合用于性别选择的精子分选的改进的授精系统包括实现超排卵，然后用性别胚胎多次胚胎生产的系统。 这些系统与其他技术相结合，包括用于增强鞘液（3）的技术和使精子细胞应激最小化的其他策略（18），以及潜在的2.9％的牛类柠檬酸钠鞘溶液和牛配子介质 马种。 描述改进的收集系统（14）和该方法的技术，使得现在可以在现场实现精子样品的商业应用以及所得到的动物。