摘要:
Disclosed is a device, suitable for use as an acoustic resonator, including a base adapted to be coupled to at least one acoustic wave generator, a spacer including an aperture and a reflector, wherein the base includes a protruding part having a thickness t; the aperture of the spacer is complementary to the protruding part of the base; the device further includes a housing having an aperture complementary to the protruding part of the base and wherein the inner edge of the aperture has the same thickness t than the protruding part; and the housing is positioned between the spacer and the reflector, such that the thickness of the inner edge of the spacer defined the thickness of a cavity between the protruding part and the reflector. Also disclosed is a method of trapping particles in a fluid.
摘要:
The invention relates to a microfluidic system in which the droplets flow through an off-chip delay line in a linear sequential order. This ensures that all the droplets are incubated for the same amount of time and under the same conditions as they flow through the delay line. The invention further relates to the use of this microfluidic system for high throughput screening methods or in vitro evolution methods.
摘要:
[Object] Provided are a particle imaging apparatus and a particle imaging method capable of taking an image of an imaging target particle with a high quality while maintaining a processing speed of a measurement apparatus. [Solution] A particle imaging apparatus 10 includes: a flow path 100 which includes a first flow path section 110, and a second flow path section 120 and a third flow path section 130 that are connected downstream of the first flow path section 110, and which causes a measurement sample including particles to flow; a particle detection unit 20 that detects a particle that flows in the first flow path section 110; a particle sorting unit 30 capable of adjusting a flow direction of the particle flowing in the first flow path section 110 such that the flow direction is selected from among at least a direction toward the second flow path section 120 and a direction toward the third flow path section 130, based on a result of detection by the particle detection unit 20; and a particle imaging unit 50 that takes an image of a particle that flows in the second flow path section 120.
摘要:
A particle analyzer, comprising a source of a beam of pulsed optical energy; a detector comprising a number of spectral detection channels to detect optical signals resulting from interactions between the beam and particles in a sample (such as, e.g., fluorescence signals), and to convert the optical signals into respective electrical signals; optical paths from the source to the sample and from the sample to the detector; a flowcell connected with the optical paths and with a flow path for a suspension of particles; a signal processing module capable of: receiving the electrical signals from the detector; mathematically combining individual decay curves in the signals into a decay supercurve; allocating individual components of the supercurve to discrete bins of predetermined time constants; and quantifying the relative contribution of individual components to the supercurve; a particle sorting actuator; an actuator driver; and at least one particle collection receptacle.
摘要:
Eine Vorrichtung zum Dispensieren von Partikeln (10) in frei fliegenden Flüssigkeitstropfen besitzt eine Fluidkammer (14), die mit einer Düse(16) fluidisch gekoppelt ist, einen Schallgeber (18), der konfiguriert ist, um in der Fluidkammer ein akustisches Feld zu erzeugen, durch das Partikel in einer Flüssigkeit in der Fluidkammer in einer Anordnung ausgerichtet werden können, und einen Drop-on-Demand-Mechanismus (20), der konfiguriert ist, um gezielt einen einzelnen Flüssigkeitstropfen (12), der einen oder mehrere Partikel enthält, zu einem gewählten Zeitpunkt aus der Düse zu dispensieren. In einem ersten Modus wird ein akustisches Feld erzeugt wird, bei dem zumindest ein Partikel mit jedem Tropfenausstoß schrittweise Richtung Düse bewegt wird, und in einem zweiten Modus wird ein akustisches Feld erzeugt, bei dem zumindest ein Partikel in der Fluidkammer festgehalten wird.
摘要:
Extracting and concentrating particles from a first fluid sample includes: providing the first fluid sample to a fluid exchange module of a microfluidic device, providing a second fluid sample to the fluid exchange module, in which the first fluid sample and the second fluid sample are provided under conditions such that particle-free portions of the first fluid sample are shifted, and an inertial lift force causes the particles in the first fluid sample to cross streamlines and transfer into the second fluid sample; passing the second fluid sample containing the transferred particles to a particle concentration module under conditions such that particle-free portions of the second fluid sample are shifted, and such that the particles within the second fluid sample are focused to a streamline within the particle concentration module.
摘要:
Devices for separating materials from a host fluid are disclosed. The devices include a flow chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer and reflector create an angled acoustic standing wave oriented at an angle relative to the direction of mean flow through the flow chamber. The angled acoustic standing wave results in an acoustic radiation force having an axial force component that deflects the materials, so that the materials and the host fluid can thus be separated. The angled acoustic standing wave can be oriented at an angle of about 20° to about 70° relative to the direction of mean flow through the flow chamber to deflect, collect, differentiate, or fractionate the materials from the fluid flowing through the device at flow rates of about 400 mL/min up to about 700 mL/min.
摘要:
A fluid handling and delivery system useful in generating a fluid stream (7) in the flow path (8) of microfluidic device (16). The system comprises a variable volume container (1) having a flexible wall (2) which acts on an amount of fluid (3) within the variable volume container (1) in response to an amount of pressure (4) exerted on the exterior surface (5) of the flexible wall (2) by an amount of gas (6). The amount of pressure (4) exerted on the exterior surface (5) of flexible wall (2) continuously adjusts the volume of the variable volume container (1) to act on the amount of fluid (3) to generate a fluid stream (7) in the flow path (8).
摘要:
Systems and methods of using the same for sorting cells are provided. The systems may include a cell sorter including a deflector capable of deflecting an analyzed droplet to a deflected droplet receiving location, a support stage including a container and configured to continuously move in two dimensions. The systems and methods of using the same find use in a variety of different applications.